Refini ng Margari ne Crypthecodinium cohnii: A natural DHA Factory •20 % lipid content by weight which is 40 % DHA •Facultative heterotroph, which does not perform photosynthesis •Growth rate of 0.0375 h -1 on glucose substrate •Uses glucose as a carbon source •Uses dead yeast from ethanol bottoms as a nitrogen source •Requires dissolved oxygen for optimal growth •Grows in brackish (saline) water. Fermentati on Travis Dallas Eric Graves Joaquin Martinez Chris McNinch Ramune O. Meskyte Charu Saini Mentors: Dan Rusinak, Jeffery Perl, John Micheli •Lack of DHA is associated with poor or slower retinal development and brain development •Helps infants gain weight faster •Recommended 300 mg/day for pregnant or lactating women •A lack of DHA is associated with Alzheimer’s disease •Inhibits the growth of human colon carcinoma cells •Increases the efficacy of chemotherapy •Infant Health •Adult Health C 22 H 32 O 2 INGREDIENTS: INTERESTERIFIED SOYBEAN OIL, WATER, ALGAE OIL, NATURAL SEA SALT, MONO/DIGLYCERIDES, NATURAL LECITHIN, SORBIC ACID, BETA- CAROTENE (VITAMIN A), ERGOCALCIFEROL (VITAMIN D), DIACETYL (FLAVORING) Degumming Bleaching Removal FFA Deodorization Centrifuge Crude SBO Citric Acid Gum Water Citric Acid Citric Acid Acid Activated Clay Enzyme Acid Activate Clay Interesterification Hardfat SBO Emulsification Pasteurization Water Sea Salt Lecithin Diacetyl Mono/Diglycerides B-Carotenes Sorbic Acid Ergocaliferols Sodium methoxide Citric Acid Soft Water Sodium methoxide Citric Acid Soft Water Scraped Surface Heat Exchanger Packaging Refrigerant To Wholesaler Dissolved Air Floatation Fermentation Centrifuge CO 2 Lysing Glucose Ethanol Still Bottom Antifoam Water Algae Glucanex Steam Cooling Water Sterile air Vent Water Air CO 2 Water NaOH Steam Steam Salt CIP System Dryer Algae Cake to Wholesaler Docosahexaenoic Acid HO O Main Ferment er Cooli ng Water Sparged Steam Vented Air 13 Sterile Air Sterile Glucane x Ster ile Anti - foam 7 8 11 Filte r 1 Filter 2 P- 8 Filte r 3 TIC 14 Compressor 1 Air PI C PIC FIC QIC QIC 9 P- 3 TIC 10 QIC Vis c Foam QIC P- 6 Turbidity QIC QIC HX-1 Fermentation Controls 35,000 gallon Fermentor Height 50 feet Diameter 12 feet Total Project Capital Cost 37,328,232.77 USD Operating Costs 18,962,032.85 USD/Year Raw Materials 6,031,280.00 USD/Year Utilities 3,532,157.83 USD/Year Maintenance 2,030,000.00 USD/Year Operating Labor Costs 2,828,000.00 USD/Year Operating Charges 707,000.00 USD/Year Plant Overhead 2,429,000.00 USD/Year G and A Costs 1,404,594.96 USD/Year Total Product Sales 25,963,238.43 USD/Year Payback Period 5.7 Years Net Present Value 84,727,200.17 USD Internal Rate or Return 25.5% -$50 $0 $50 $100 $150 $200 0 5 10 15 M illions Cumulative Cashflow Cumulative Cash Flow Basis: 1% of Market Year of Plant Life -$100 -$50 $0 $50 $100 $150 $200 $250 $300 $350 0 5 10 15 M illions Cumulative Cashflow Cumulative Cash Flow Basis: 2% in 6 Years Year of Plant Life -$100 -$50 $0 $50 $100 $150 $200 $250 $300 $350 $400 0 5 10 15 M illions Cumulative Cashflow Cumulative Cash Flow Basis: 2% of Market Year of Plant Life Expand to 2% Market Share in 6 Years Payback Period 7.13 Years Net Present Value 158,394,244.01 USD Internal Rate or Return 34.5% 2% Market Share from Start Payback Period 5.00 Years Net Present Value 198,627,853.09 USD Internal Rate or Return 34.5% Growth Phase Maintenance What is Sanitary Design? Preventing microbial growth and simply being clean How do you go about this? Physical separations of the different plants All equipment composed of stainless steel Margarine Plant composed of polished stainless steel Linear and horseshoe shaped plant design Clean in Place system (CIP) on the inside Spray cleaning foam on the outside Who cares? You do, and so do we. We do not want you getting sick from our product. Algae Oil Alpha Algae Co. 1928 SE Otis Rd. Cedar Rapids, IA 52401 Alpha Algae Co. Alpha Algae Co. Truck Engines Just Spread It On! Sooo Healthy! 0g Trans Fatty Acids 100% Vegan 100% Daily Omega-3 Fatty Acids per Serving Eat Premium Golden rather than me for your essential fatty acids. Butter your biscuits! We like it clean ! You should get DHA from algae just like me. It is tons healthier for both of us. Algae Growth Cycle 0 5 10 15 20 25 30 0 20 40 60 80 100 Time (Hours) Cell Density (g/L)