REF : IMG 237 - AMPLITECH Imegen-CALR_EN.pdf · 2017. 11. 17. · Instituto de Medicina Genómica SL Agustín Escardino 9, Parc Científic de la Universitat de València 46980 Paterna

Instituto de Medicina Genómica SL

Agustín Escardino 9,

Parc Científic de la Universitat de València

46980 Paterna (Valencia, España)

+34 963 212 340 - [email protected]

imegen.es

Rev. 2

1

Detection of indel mutations in CALR by capillary electrophoresis

REF : IMG-237






imegen.es

Rev. 2

2






imegen.es

Rev. 2

3






imegen.es

Rev. 2

4






imegen.es

Rev. 2

5






imegen.es

Rev. 2

6

Reagents Color Quantity Storage Master Mix CALR White disk 2x408 µl -20ºC

Taq Yellow disk 25 µl -20ºC

Control CALR White Lid 33 µl -20ºC

Table 1. Components of the Imegen-CALR kit






imegen.es

Rev. 2

7

Equipments not provided

1 PCR Thermal Cycler

2 Micropipettes (10 µL, 20 µL and 200 µL)

3 Vortex

4 Capillary Electrophoresis equipment

Table 2. Materials and Equipment required but not provided

Materials not provided

1 0.2ml PCR tubes

2 Filter tips (10 µL, 20 µL and 200 µL)

3 1.5 ml sterile tubes

4 Latex gloves

5 Reagents for fragment analysis by capillary

electrophoresis






imegen.es

Rev. 2

8






imegen.es

Rev. 2

9

Parameter Stage 1 Stage 2

PCR amplification Stage 3

Cycle numbers

&

Function

1x

Initial denaturation

30x cycles 1x cycle

Denaturation Annealing Extension Final Extension and

4ºC hold

Temperature 94ºC 94ºC 60ºC 72ºC 72ºC 4ºC

Time 5 mins 1 min 1 min 2 mins 10 mins ∞

Table 3. PCR program for Imegen-CALR kit






imegen.es

Rev. 2

10






imegen.es

Rev. 2

11






imegen.es

Rev. 2

12

REF : IMG 237 - AMPLITECH Imegen-CALR_EN.pdf · 2017. 11. 17. · Instituto de Medicina Genómica SL Agustín Escardino 9, Parc Científic de la Universitat de València 46980 Paterna

Documents