11:00 ‐ 11:10 11:10 ‐ 12:00 PC01 12:00 ‐ 12:15 PC02 12:15 ‐ 12:30 PC03 12:30 ‐ 12:45 PC04 12:45 ‐ 13:00 PC05 14:30 ‐ 14:50 14:50 ‐ 15‐50 L01 15:50 ‐ 16:30 L02 16:30 ‐ 16:50 L03 16:50 ‐ 17:10 L04 17:10 ‐ 17:25 L05 17:25 ‐ 17:55 17:55 ‐ 18:35 L06 18:35 ‐ 18:55 L07 18:55 ‐ 19:15 L08 19:15 ‐ 19:30 L09 19:30 ‐ 22:00 Wednesday, March 6, 2013 Special PreConference Session Biomolecular Technology of Proteins (BioToP) Recombinant Protein Production Conference Programme Welcome address and introduction of BioToP Plenary Lecture: High throughput sequencing and bioinformatics for genome analysis of the Chinese hamster and CHO cell lines Alfred Pühler (University of Bielefeld, Germany) Characterization of model recombinant monoclonal IgM antibodies produced in CHO‐DG44 cell lines Opening Plenary Lecture: Pre‐expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E. coli Lloyd Ruddock (University of Oulu, Finland) Justyna Nocon (Boku, Wien, Austria) Recombinant production and characterization of four N. crassa lytic polysaccharide monooxygenases Daniel Kracher (Boku, Wien, Austria) Session 1: Process and product quality control Invited lecture: Directing cell line design towards built‐in process quality Claas Wodarczyk (Rentschler Biotechnologie GmbH, Laupheim, Germany) Short presentation: Metabolic characterization of MAb producing CHO cells for predictive bioprocess Chairs: Dethardt Müller (Rentschler, Germany), Diethard Mattanovich (BOKU, Vienna, Austria) Ana P. Teixeira (IBET, Oeiras, Portugal) Short presentation: New Bacillus subtilis expression strains Thomas Schweder (EMA‐Univ. Greifswald, Germany) Short presentation: Influence of single amino acid exchanges in a recombinant enzyme on host cell metabolic activity and expression Nathalie Rahmen (RWTH Aachen, Germany) Coffee Break Session 2: From Strain to Process design Invited lecture: Microbial production of novel biologics using secretory E. coli strains Chairs: Peter Neubauer (TU Berlin, Germany), Joseph Shiloach (NIDDK, NIH, U.S.A.) Guido Seidel (Wacker Biotechnologie, Jena, Germany) Short presentation: High cell density E. coli fed‐batch fermentation in a single‐use rocking bioreactor Nico M.G. Oosterhuis (Cellution, The Netherlands) Short presentation: Aspergillus niger : a cell factory with unlimited prospects Vera Meyer (TU Berlin, Germany) Short presentation: Maximising levels of secreted recombinant proteins in P. pastoris by introducing transcriptional flexibility in a high‐throughput manner Roland Weis (VTU, Austria) Poster session, exhibition and welcome reception Chairs: Reingard Grabherr & Johannes Grillari (BOKU University of Natural Resources and Life Sciences) Diethard Mattanovich (BOKU, Vienna, Austria) Peter Neubauer (TU Berlin, Germany), Dethardt Müller (Rentschler, Germany) Chair: Peter Neubauer (TU Berlin, Germany) RPP 7 Conference Welcome addresses and introduction Veronika Chromikova (Boku, Wien, Austria) Comparative analysis of virus‐like particle (VLP) production in three different insect cell lines – Sf9, High Five and Tnao38 Miriam Klausberger (Boku, Wien, Austria) Metabolic model‐based prediction of engineering targets for increased production of heterologous proteins
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Recombinant Protein Production Conference Programme · RPP 7 Conference Welcome addresses and introduction Veronika Chromikova (Boku, Wien, Austria) Comparative analysis of virus‐like
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11:00 ‐ 11:10
11:10 ‐ 12:00PC01
12:00 ‐ 12:15 PC02
12:15 ‐ 12:30PC03
12:30 ‐ 12:45 PC04
12:45 ‐ 13:00 PC05
14:30 ‐ 14:50
14:50 ‐ 15‐50L01
15:50 ‐ 16:30 L02
16:30 ‐ 16:50 L03
16:50 ‐ 17:10 L04
17:10 ‐ 17:25L05
17:25 ‐ 17:55
17:55 ‐ 18:35 L06
18:35 ‐ 18:55 L07
18:55 ‐ 19:15 L08
19:15 ‐ 19:30L09
19:30 ‐ 22:00
Wednesday, March 6, 2013
Special PreConference Session
Biomolecular Technology of Proteins (BioToP)
Recombinant Protein Production Conference Programme
Welcome address and introduction of BioToP
Plenary Lecture: High throughput sequencing and bioinformatics for genome analysis of the Chinese hamster
and CHO cell linesAlfred Pühler (University of Bielefeld, Germany)
Characterization of model recombinant monoclonal IgM antibodies produced in CHO‐DG44 cell lines
Opening Plenary Lecture: Pre‐expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic
disulfide bond containing proteins expressed in the cytoplasm of E. coliLloyd Ruddock (University of Oulu, Finland)
Justyna Nocon (Boku, Wien, Austria)
Recombinant production and characterization of four N. crassa lytic polysaccharide monooxygenases
Daniel Kracher (Boku, Wien, Austria)
Session 1: Process and product quality control
Invited lecture: Directing cell line design towards built‐in process quality