Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms Useful products Gene for pest resistance Gene for toxic-cleanup bacteria Some uses of prot Protein for “stone-washing” jeans Protein for dissolving clots Some uses of genes:
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Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms.
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Recombinant DNA technologyDNA isolated from
two sources and cut by the same enzyme
Gene of interest
RecombinantDNA
Plasmid(vector)
Transgenic organisms
Useful products
Gene for pestresistance
Gene fortoxic-cleanup
bacteria
Some uses of proteins:
Protein for“stone-washing”
jeans
Protein fordissolving
clots
Some uses of genes:
Restriction Endonucleases
Recombinant DNA technology
First DNA Cloning Experiment
Cohen et al., 1973
“Recombinant DNA”
Recombinant DNA technology
Cloning Vectors: pBR322 plasmid
Recombinant DNA technology
Genetically Modified (GM) FoodsIn the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified.
●Corn has been genetically modified to resist insect
infestation.
Golden Rice
Genetically Modified (GM) Foods
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615