receptor: Application in live cell imaging. Supporting ... · 1 Supporting Information “Turn-On” fluorescent chemosensor for Zinc (II) dipodal ratiometric receptor: Application
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1
Supporting Information
“Turn-On” fluorescent chemosensor for Zinc (II) dipodal ratiometric receptor: Application in live cell imaging.
Kundan Tayade a,b, Banashree Bondhopadhyayd Hemant Sharmac, Anupam Basud, Vikas Gitea, Sanjay Attardeb, Narinder Singh*c, Anil Kuwar*a aSchool of Chemical Sciences, North Maharashtra University, Jalgaon 425 001 (MS) India.bSchool of Environmental and Earth Sciences, North Maharashtra University, Jalgaon 425 001 (MS) India.cDepartment of Chemistry, Indian Institute of Technology, Ropar, Rupanagar (Punjab) India.dMolecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, India. ----------------------------------------------------------------------------------------------------------
Figure S5a. TGA of receptor 2 (heating rate 10 per minute under nitrogen ℃
environment).
Figure S5b. TGA of receptor 2.Zn2+ (heating rate 10 per minute under nitrogen ℃environment).
5
Figure S6a. DSC of receptor 2.
Figure S6b. DSC of receptor 2.Zn2+.
6
Figure S7a. Fluorescence titration spectra of receptor 2 (0.1 mM) in the presence of
different concentrations of Zn2+ (1 mM) (λex = 278 nm, λem = 341 nm, excitation and
emission slit 5 nm) up to 10 µl (0.05 equiv.).
Figure S7b. Fluorescence titration spectra of receptor 2 (0.1 mM) in the presence of different concentrations of Zn2+ (1 mM) (λex = 278 nm, λem = 341 nm, excitation and emission slit 5 nm) up to 400 µL (2 equiv.).
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0 0.200000003
0.400000006
0.600000009
0.800000012
1.000000015
1.200000018
1.400000021
1.600000024
0.00E+001.00E+07
2.00E+07
Equivalent of Zn2+ ion added
Inte
nsity
(cps
)
Figure S7c. Mole ratio plot (λem = 341 nm) illustrating the changing stoichiometry from 2:1 to 1:1.
Zn(II)N
a(I)
K(I)
Mg(II)
Al(III)
Cs(I)Ba(II)Ca(II)Sr(II)Cr(III)M
n(II)Fe(III)Co(II)N
i(II)Cu(II)Cd(II)H
g(II)Pb(II)Th(IV
)A
g(I)Bi(III)U
(VI)
-2-1
01
23
45
Various Metal Ions
ΔF/F
0
Figure S8. Fluorescence spectrometric response (ΔF = F−F0) of receptor 2 (0.1 mM) upon addition of 100 µL of respective cation salts (1 mM) in CH3CN/H2O.
8
Zn(II)N
a(I)K
(I)M
g(II)A
l(III)Cs(I)Ba(II)Ca(II)Sr(II)Cr(III)M
n(II)Fe(III)Co(II)N
i(II)Cu(II)Cd(II)H
g(II)Pb(II)Th(IV
)A
g(I)Bi(III)U
(VI)
0.00E+001.00E+07
2.00E+07
Competating Ions Competating Ions + Zn(II)
Various Metal Ions
Inte
nsity
(cps
)
Figure S9. A fluorescence sensing of Zn2+ ion (1 mM, 1equiv) by receptor 2 (0.1 mM) in the presence of other competing cations (1mM, 2 equiv.).
Figure S10a. Job’s plot representing the stoichiometry of complex 2.Zn2+ (host : guest;
Figure S10c. Fluorescence intensity at 341nm of receptor 2 (0.1 mM) versus increasing concentration of Log[Zn2+]. The fluorescence response fits to a Hill coefficient of 1.1446, which is in concordance with the 1:1 binding stoichiometry for the receptor 2.Zn2+ complex.
10
Figure S11. LC-MS spectra of 2.Zn2+ complex (M+H+)
0 20000
40000
60000
80000
100000
120000
05E-8
0.0000001
1/[G]
1/∆F
Figure S12a. A Benesi-Hildebrand methodology for receptor 2, (1/∆F) vs 1/[G], Ka = 1.29 × 106 M-1.
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0.00E+00
2.00E+06
4.00E+06
6.00E+06
8.00E+06
1.00E+07
1.20E+07
05E+11
1E+121.5E+12
∆F
∆F/[G
]
Figure S12b. A Scatchard methodology for receptor 2, ∆F/[G] vs ∆F, Ka = 1.00 × 106
M-1.
0 1 2 3 4 5 6 7 8
-1000000-500000
0
F/F0
(1-F
/F0)
/[G]
Figure S12c. Connor’s fitting method for receptor 2, (1-F/F0)/[G] vs F/F0, Ka = 1.94 × 106 M-1