Real time analysis of biomass fermentation using Raman spectroscopy Wesley Thompson 1 , Shannon Ewanick 2 Renata Bura 2 , Brian Marquardt 1 Applied Physics Lab 1 Department of Forest Resources 2 University of Washington University of Washington March 23, 2011
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Real time analysis of biomass fermentation using Raman ......Real-time Analysis of a Hydrolysate Fermentation Process ¨900 mL hydrolysate was fermented with yeast for 8 hours ¨Additional
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Real time analysis of biomass fermentation using
Raman spectroscopy
Wesley Thompson1, Shannon Ewanick2
Renata Bura2, Brian Marquardt1
Applied Physics Lab1 Department of Forest Resources2
University of Washington University of Washington
March 23, 2011
Bioethanol
BiodieselBiobutanol
Biopolymers
Biochemicals
Hydrogen
BiomethanolBiogas
Bio-oil
XylitolBio-adhesives
Fractionation
Biomass Selection
n Starting biomass
¨Cellulose, hemicellulose, lignin
n Pretreated substrates
¨ Solids: Cellulose, hemicellulose, lignin
¨ Liquid: Glucose, hemicellulosic sugars, inhibitorsn Hydrolysate liquor will be our focus
Components To Be Measured
n Sampling
n Analytical characterization
¨ Sensors
¨ Instruments
n Data handling
¨ Sensor fusion
¨ Multivariate modeling
n Process modeling
n Process optimization – feedback and feed forward control
Bioprocessing Challenges
Real-time Analysis of a HydrolysateFermentation Process
¨ 900 mL hydrolysate was fermented with yeast for 8 hours
¨ Additional sugar was added to the hydrolysate
n 30 g/L glucose and 15 g/L xylose added broth (which already contained ~ 5g/L of each)
¨ The fermentation broth was held at 30°C and pH 6 for the duration of experiment.
¨ Raman spectra were collected every 1 minute
n 3/8” ballprobe with sapphire optic, 215mW power at probe tip
n Average of six, 10 second exposures
¨ HPLC Samples
n Collected a 1mL aliquot every 10 minutes during fermentation
Lignin Fluorescence Data Pretreatment
n Fluorescence is a different optical process than Raman spectroscopy¨ Can be removed while