Forensics Magazine Webinar: May 8, 2014 Presenters: Rapidly Detect, Identify, and Monitor Unknown Substances - Anywhere Alan Rein Thomas Szakas Agilent Technologies Agilent Technologies For Forensic Use
Forensics Magazine Webinar: May 8, 2014
Presenters:
Rapidly Detect,
Identify, and Monitor
Unknown Substances
- Anywhere
Alan Rein Thomas Szakas Agilent Technologies Agilent Technologies
For Forensic Use
At-Site Analysis Supports Forensic Investigations
•Aid in establishing probable cause, allowing seizure
of material for further analysis
•Reduce the audit trail and shorten the chain of evidence
•Enable real-time decisions about what to analyze and where to sample
•Identify the nature of a threat and establish perimeters for responders and civilians.
•Assess the progress of on-going decontamination operations and confirm
remediation of the incident site.
•Aid personnel involved in border control, corporate safety, mail room and post
office security
•Support the identification and seizure of counterfeit pharmaceuticals, illicit drugs
and related chemicals
•Analyze toxic industrial chemicals, chemical agents, white powders, and explosives
For Forensic Use
Agilent Mobile GC-MS and FTIR Analyzers – Powerful tools for at-site Forensic Analysis
•Rapid, reliable analysis of unknown compounds and materials is critical in forensic,
environmental, homeland security and military applications.
•Agilent provides key technologies that are optimized for at-site analysis and enable
sampling of solids, liquids, air, gels, foods, and even blood.
•Agilent's mobile GC/MS, and FTIR analytical instruments, enable lab quality results
in the field.
In the homeland security and military fields rapid detection and identification
of chemical warfare agents (CWAs) and toxic industrial chemicals (TICs) is
essential.
In forensic and investigative fields, the speed and accuracy of results are
critical. Test for poisons in a forensics investigation, analyze explosives and
their residues, check a crime scene for illicit drugs, precursor chemicals and
cutting agents.
For Forensic Use
Agilent At Site Analyzers for Forensic Analysis
5975T LTM GC/MSD 4500 Portable FTIR
4300 Handheld FTIR
For Forensic Use
June 11, 2014
Agilent 5975T GC/MSD & 4500/4300 FTIR
Agilent At Site Forensic Analyzers – Cost effective and
Well Suited to At-Scene and Field Lab Use
For Forensic Use
IR Spectroscopy Provides
Each structural characteristic of a molecule has a unique vibrational frequency that can
identify the functional group present. When all the different functional groups in a molecule are
simultaneously measured, a unique molecular "fingerprint" provides the chemical identity of a
sample.
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3950 3900 3850 3800 3750 3700 3650 3600 3550 3500 3450 3400 3350 3300 3250 3200 3150 3100 3050
0.22 0.20 0.18 0.16 0.14 0.12 0.10 0.08 0.06 0.04 0.02 0.00 -0.02 -0.04 -0.06 -0.08
Wavenumber
Ab
so
rban
ce
Spectral Identification
of Unknowns
Quantitative analysis of
sample components
For Forensic Use
FTIR Analyzers for At-Site Forensic Analysis
4500a Portable FTIR
• 15 lbs (6.8 kg)
• detachable PDA computer
• Internal battery
• ATR sample interface
• For field use in non-hot zones
• Cost effective
For Forensic Use
4500a Series FTIR Sample Interface
ATR • Simple, easy to use
- <2mg sample placed on sensor,
- pressure device ensures contact,
- answer provided in <30 secs.
• Short path length ~2 µm- Library match, product identification
• Uses Diamond sensor- Chemical and scratch resistant
- Internal reflection
For Forensic Use
FTIR Forensic Analyzer Software
• Method Driven
• Focused on Results
• Saves original data
• Synchronizes with PC
software
• Completely functional
software for Point of
Measurement Applications
• Quantitation
• Qualitative Library Search /
Identification
13,000 spectra from a comprehensive list of:
Bio-chemicals
Forensics
White powders
Hazardous and Toxic Chemicals
HPV chemicals
Food additives
Explosives
For Forensic Use
Identification of IIlicit Drugs
Agilent 4500a PDA computer controls all operations of the system.
After spectra of an unknown is acquired, a pre-loaded method
automatically searches an on-board forensic substance library and
displays both the identity of the unknown, the quality of the match as
well as the spectrum of the material
For Forensic Use
Identification of IIlicit Drugs
• Spectra of street drugs searched
against the Agilent Forensic Library.
• Search reveals:
spectrum of the unknown
sample
spectrum of best match
sample identity
numerical quality of the
match.
For Forensic Use
Identifying Components in Drug Mixture
• Spectral search result of a sample
suspected of containing cocaine.
The spectral search indicates a
mixture of cocaine,
acetophenetidine and p-
acetophenetidine. The quality
index is low due to the mixture of
the two main components.
• With residual search, the first
match is subtracted and the
residual spectrum is automatically
searched against the library.
• The residual spectrum matches
well with cocaine based (crack)
and the match quality is quite
good.
Search After Spectral
Subtraction of Best Match
from Drug Sample
Red – Spectrum of Drug Sample
Blue - Spectrum of Best Match
For Forensic Use
FTIR Analyzers for At-Site Forensic Analysis
4300 Handheld FTIR
• 4.8 lbs (2.2 kg)
• Integrated computer
• “Hot swap” internal
batteries
• Selectable sample
interfaces
• For field use in non-hot
zones
ATR - for solids, liquids, pastes and gels. Diffuse Reflectance - for scattering samples
For Forensic Use
Spectra of Motorcycle and Truck Components
Pillion case
Truck Paint
Pillion Case with
residual Truck Paint
For Forensic Use
Gas Chromatography - Mass Spectrometry GCMS
1. What is GC/MS and what does it tell us?
2. Advances in technology to ‘bring it to the field’
3. Threat Detection by flexible sampling techniques
4. Relevent data
5975T GC/MSD
Mobile, Portable,
Transportable….
For Forensic Use
5975T:Mobile Lab Process Savings
Ship sample Sample
Prep Test
Data Processing
Fixed Lab
Sample Prep
Test Data
Process
Mobile Lab
For Forensic Use
A Fast and Simple alternative probe for analyzing solid, liquid, slurry and
vapor samples in the field.
TSP: Fast and Simple Sample Prep
For Forensic Use
1. Probe
2. TSP Adapter
3. Inlet liner
4. TSP sample
vial
How does the TSP work?
Steps:
1.Place a small amount of liquid or solid in
the sample vial
2.Load vial into probe
3.Insert probe into inlet via TSP adapter
4.Re move probe and discard vial
For Forensic Use
Capillary-Trap Sampler(CTS) for Vapor Sampling
A fast and simple to use gas sampler for trace level target
compounds (ppb~ppm) sampling in field.
Gas
pump Trap
head
Trap
columns
For Forensic Use
How does the CTS Works?
1. Install trap column into CTS head
2. Pump air through trap columns 0.5~2Min to trap target compounds
• Up to 6 unique samples may be collected with one sampler
3. Remove the columns to TSP vial
4. Run analysis by TSP
For Forensic Use
Ultra-fast fire accelerants analysis of fire debris with 5975T+CTS+TSP
• Ultra fast in sampling with CTS: 1min
• Ultra fast in 5975T+TSP analysis: 5min
• Ultra fast in data handling: 1min
For Forensic Use
TIC Contrast: Save Time for Gasoline Confirmation
Page 25
5975T GCMS:
5min
Typical Lab
GCMS:40min
For Forensic Use
Rapid Pesticide Residue Identification with 5975T + TSP
Simplified QuEChERS method w/o Clean-up
TSP: the RSD% of all the compounds is from 2.3 ~7.8% with 1ul injection
Running time <5 Min
For Forensic Use
RT Compounds
1.8765 Barbital
2.3049 Amobarbital
2.4384 Secobarbital
3.1265 Cocaine
3.2050 1-Piperidinepropanol, .alpha.-cyclopentyl-.alpha.-phenyl-
3.2407 Promethazine
3.2788 SKF525
3.3232 Oxazepam
3.4365 Lorazepam
TIC of Drugs
Standard
3.4782 Diazepam
3.563 Chlorpromazine
3.5645 Chlorprothixene
3.5950 Chlordiazepoxide
3.9862 Papaverine
4.024 Clozapine
4.0243 Clonazepam
4.0971 Estazolam
Rapid Drug Identification by 5975T+TSP
For Forensic Use
Rapid Explosives Identification by 5975T + TSP
Soil matrix,SQCI-011;
1,2,3:2-Nitrotoluene,3-nitrotoluene,4-
nitrotoluene; 4: PETN; 5:1,3-Dinitrobenzene;
6: 2,4-dinitrotoluene; 7: 2,6-dinitrobenzene;
8,9,10: TNT mixture; 11: RDX; 12 3,4,5-TNT;
13: 2-Amino-4,6-dinitrotoluene;
14 :4-amino-2,6-dinitrotoluene
1
2
3
4
5 67
8
9 10
11
12
13 14
Running time< 5Min
No sample preparation needed, just measure sample powder
Fast
Temperature
Program
method
For Forensic Use
So, how does all this technology fit together?
June 11, 2014
Confidentiality Label
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For Forensic Use