R dna

An Assignment on-An Assignment on-

RECOMBINENT DNA TECHNOLOGYRECOMBINENT DNA TECHNOLOGY

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Deoxyribonucleic acid (DNA) is a long double-stranded chain of nucleotides

Deoxyribonucleic acid (DNA) is a long double-stranded chain of nucleotides

• DNA is made up of four nucleotides: A, C, G,

and T.

• A always pairs with T.

• C always pairs with G.

• The two strands of DNA are in an antiparallel

configuration.

• DNA is made up of four nucleotides: A, C, G,

and T.

• A always pairs with T.

• C always pairs with G.

• The two strands of DNA are in an antiparallel

configuration.

Recombinent DNA:Recombinent DNA:

Techniques in which DNA fragments are linked to self-replicating vectors to create recombinant DNA molecules which are replicated in a host cell.

Techniques in which DNA fragments are linked to self-replicating vectors to create recombinant DNA molecules which are replicated in a host cell.

Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNALink: Link: http://en.wikipedia.org/wiki/Recombinant_DNA

Making recombinant DNA:

Making recombinant DNA:

Cut with restriction enzymesCut with restriction enzymes

Isolate DNA Isolate DNA

Ligate into cloning vector Ligate into cloning vector

Transform recombinant DNA molecule into host cellTransform recombinant DNA molecule into host cell

Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNA

Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNA

Why is rDNA important? Why is rDNA important?

Better Crops (drought & heat resistance)Recombinant Vaccines (ie. Hepatitis B) Prevention and cure of sickle cell anemiaPrevention and cure of cystic fibrosis Production of clotting factors Production of insulin Production of recombinant pharmaceuticals Plants that produce their own insecticides Germ line and somatic gene therapy

Better Crops (drought & heat resistance)Recombinant Vaccines (ie. Hepatitis B) Prevention and cure of sickle cell anemiaPrevention and cure of cystic fibrosis Production of clotting factors Production of insulin Production of recombinant pharmaceuticals Plants that produce their own insecticides Germ line and somatic gene therapy

• Recombinant DNA is widely used in biotechnology, medicine and research.

• Golden rice: a recombinant variety of rice that has been engineered to express the enzymes responsible for β-carotene biosynthesis.

• Herbicide-resistant crops.

• Insect-resistant crops.

• Recombinant Chymosin: Found in Rennet, is an enzyme required to manufacture cheese.

• Recombinant DNA is widely used in biotechnology, medicine and research.

• Golden rice: a recombinant variety of rice that has been engineered to express the enzymes responsible for β-carotene biosynthesis.

• Herbicide-resistant crops.

• Insect-resistant crops.

• Recombinant Chymosin: Found in Rennet, is an enzyme required to manufacture cheese.

Link: Link: http://en.wikipedia.org/wiki/Recombinant_DNA

INSULIN for diabetics FACTOR VIII for males suffering from hemophilia A FACTOR IX for hemophilia B Human growth hormone (HGH) Erythropoietin (EPO) for treating anemia Several types of ‘Interferons’Several ‘Interleukins’ Granulocyte-macrophage colony-stimulating factor

(GM-CSF) for stimulating the bone marrow after a bone marrow transplant

Parathyroid hormone

INSULIN for diabetics FACTOR VIII for males suffering from hemophilia A FACTOR IX for hemophilia B Human growth hormone (HGH) Erythropoietin (EPO) for treating anemia Several types of ‘Interferons’Several ‘Interleukins’ Granulocyte-macrophage colony-stimulating factor

(GM-CSF) for stimulating the bone marrow after a bone marrow transplant

Parathyroid hormoneLink: Link: http://en.wikipedia.org/wiki/Recombinant_DNALink: Link: http://en.wikipedia.org/wiki/Recombinant_DNA