1 Serial Number:___________ Owner:___________________ Queen Station tm User Manual Apis Engineering, LLC www.apisengineering.com
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
1
SerialNumber:___________
Owner:___________________
QueenStationtmUserManual
ApisEngineering,LLC
www.apisengineering.com
2
Chapters1. Introduction2. Assembly/Disassembly
a. Step1BasePostb. Step2PostLockc. Step3MicroscopeBoomd. Step4Microscopee. Step5Lightsf. Step6Co2Cartridgeg. Step7VentralHook,StingForceps,SyringeSlideh. Step8Disassembly
3. CareandCleaninga. Baseb. Microscopec. BubbleCounterd. SyringeStage(slide)e. DorsalandVentralhooksf. Syringeg. Lights
4. Co2Systema. 74gCartridgeInstallation/Removalb. PressureAdjustmentProcedurec. BubbleCounter
i. WaterFillii. Adjustmentiii. Draining
d. TemporarilyShuttingOffCo2Flowe. LongTermShuttingOffCo2Flow
5. InstallingQueeninQueenHolder6. SecondaryAnesthetizationofQueens7. LightingSystem
a. Installation/Removalb. Alignmentc. IntensityAdjustment
8. Storagea. PackinginTravelCase
9. Accessories10. Schematics
3
Section1Introduction
ThismanualisintendedtoassisttheuserwiththebasicfeaturesoftheQueenStationtminstrumentalinseminationdevice.Thismanual,however,isnotintendedtobeatrainingmanualon“how”toinseminatequeens.Itisincumbentupontheusertoattendatrainingsessiononqueeninsemination.
Thisinstrumentrepresentsthousandsofhoursofresearchanddevelopmentincludinginputfromuniversity,professionalandqueenbreedingorganizations.Youcanbeassuredthatyouhavepurchasedthefinestinstrumentavailableanywhereintheworldtoday.
TheQueenStationtmisvirtuallymaintenancefreeandwithpropercleaning,useandstorageitwilllastalifetime.Someofthefeaturesincludedwiththisdeviceareuniqueandareunderpatentpendingprotection.
TheadvantagesoftheQueenStationtmarelistedhere:
1. LargeindustrialCo2cylindersarenolongerneeded.Ifyoutraveltoinseminatequeensyoucantakethe74gCo2cartridgesandnothavetoworryabouttheavailabilityofCo2.TheindustrialCo2cylindersareinherentlydangerousandhaveseriouslyinjuredpeople.Wehavesuccessfullyinseminatedmorethan100queensonone74gramcartridgeandhadenoughCo2remaininginthecartridgeforanother100queens.
2. Theon-boardgoosenecklightsarestate-of-the-artunitsthatarecontrolledwithamicroprocessordimmersystem.Theselightscompletelyreplacethebulkyandexpensiveunitscurrentlyneededwithdevicesofferedbyothervendors.Theselightsruncoolandcanbepositionedwherevertheuserdesires.
3. ABubblecounterthatactuallyallowstheusertocountaccuratelysizedbubbles.Withthisinstrumentthereisnolongeranyneedforglassbeakers,hoses,stoppersandclutterassociatedwitholdersystems.OldersystemsusingbeakerscannotcontrolthebubblesizeandhencecannotcontrolthetrueamountofCo2beingdispensedtothequeenholder.
4. Superiorsyringestage(slide).Allothercommerciallyavailableunitsfeatureaninferiorsyringestage.ThesyringestageontheQueenStationtmisalaboratorygradeunitthatallowstheusertoadjustthetensionasheorsheseefit.
5. Ballsocketsthatpresenteventensioninallareasofrotation.Otherunitshaveaseriousflawthatcausesthetensiononthedorsalandventralhookstobehigherinonedirectionofrotationthantheother.Ourunithassmooth,eventensioninalldirections
4
makingthedamageofqueensunlikely.
6. Fixeddorsalandventralhooklocationsrelativetothequeenholder.Otherunitshaveadjustablepoststhatholdthehooks.Thiscausesissueswithoperatorswhomaynotbeabletofindtheperfectlocationandangleneededtoinseminatethequeen.Withfixed
7. Boomtrinocularmicroscopebuiltintotheunit.Thissavesspaceontheworkbenchandeliminatesyetanothersetupproblemfortheoperator.
8. Hardanodizedfinishonmostparts.Hardanodizeisharderthanstainlesssteel,dielectricandstainresistant.Thisequatestoeaseofcleaningandmaintenance.
9. Optionalhardshippingandstoragecontainermakestransportingtheuniteasy.
Section2Assembly
Step1
AftercarefullyunpackingallcomponentsfromPelicancase,placethebaseunitinthelocationwhereyouwillbeinseminatingqueens.Note:thebaseunithasthreeSorbothanevibrationisolationpadsonthebottom.Thesepads,whennew,areverystickyandassuchmakeitdifficulttoshifttheunit.Placingitinitsproperlocationisthebestpractice.Onceinproperlocation,placethemicroscopeboomcolumn(threadsfirst)intotheshaftlocklocatedattherearofthebase.Presstheshaftlockclosed
Figure1Properlocationofboomcolumn,
5
Step2
Nextinstallboomcolumnshaftlockat6”upfromtopofbase.Oncethismeasurementisestablishedtheshaftlockcanremainontheshaft.ThePelicancasefoamisrecessedforthislockandsubsequentuseswillnotrequirethisadjustment.
Figure2Boomcolumnshaftlocklocation
Step3
Installmicroscopeboomontotheboomcolumnasshowninfigure3.Theproperassemblytechniqueistomounttheboomtotheleftsideofthecolumn.Mountinginthisfashionallowstherightsideofthebasetoremainrelativelyopen.
Figure3ProperBoominstallation
Step4
6
Nextthemicroscopeheadcanbeinstalledintoitssupportring.Gentlyplacethemicroscopeintotheringandtightenthethumbscrewontherightsidetoholditinplace.Theoptionalcameracanbeinstalledatthistime.
Figure4Microscopeinstallation
Step5
Figures5and6showthesuggestedmethodofroutingthelampflexibleshafts.Togainmaximumilluminationthelightsneedtobeaimeddirectlyontothequeenholder.Routingaroundthemicroscopeadjustmentknobskeepsthemoutofthewayofthesyringeslide.
Figure5Microscopelightsproperlyrouted
7
Figure6Microscopelightsproperlymounted
Step6
NextCo2cartridgecanbeinstalled.Screwitcompletelyontotheregulator.Note:Pleasereadthesection4inthismanualpriortoinstallation
Figure7Co2Cartridgeinstallation.
Step7
Installthepolycarbonatequeenholderontothequeenholderpost.MakesuretheO-ringsareinstalledonthepost.Nextinstalltheventralhookpostandventralhookintotheleftshaftlock.Positiontheventralhooksuchthatitislevelwiththetopofthepolycarbonatequeenholder.Insimilarmannerinstallthestingforcepspostandstingforcepsontherightside.Thesyringeslideandsyringecannowbeinstalledontherightside.Positionthestingforcepssuchthatitislevelwiththetopofthepolycarbonatequeenholder.
8
Figure8Queenholder,ventralhook,stingforcepsandsyringeslideinstalled
Step8
Disassemblyisaccomplishedbyfollowingthereverseorderofthestepslistedabove.Note:oncethe10mmshaftlocksonthefrontpostsareproperlysettheycanremainonthepostsforquickersetuponsubsequentuses.Thefoaminthepelicancaseisrelievedfortheselocks.
Section3CareandCleaning
1. Base:Cleaningthebaseisextremelyeasy.Afterdisassemblyforstoragetheusershoulduseeitherethanol,isopropylalcoholoranalcoholbasedwindowcleanersuchasWindex.Useacleancottonclothandspraythealcoholonthecloth(notonthebase)andwipetheentireunit.
2. Microscope:Themicroscopeshouldbecleanedinthesamemannerasthebase.Themicroscopelensesandeyepiecesshouldbewipedcleanbeforestoring.
3. BubbleCounter:Thebubblecounterexteriorcanbecleanedwithethanoloralcoholonacleanragbutbesuretowipethepolycarbonatetubewithadrycloth.Thiswillpreventthealcoholfromcrazingthesurfaceofthetube.
4. SyringeStage(slide):Theexteriorofthestagecanbecleanedwiththeethanoloralcoholclothbutitshouldbenotedthatitispossibletoalsocleantheoilfromthedovetails.Aftercleaningwithalcoholcloththeusershouldre-lubricatethedovetailsasfollows.Rotatetheslidecompletelytooneendofitsrange.Addonedropoflubricatingoil(suchas3-in-Oneoil)oneachdovetail.Slowlyrotatetheslidetotheotherendofitsrange.Wipeanyexcessoilfromthestage.Returntheslidetoitscenteroftravel
9
position.
5. DorsalandVentralHooks:Priortoeveryuse,aftereveryuseandin-betweeneveryqueeninseminationthedorsalandventralhooksshouldbesterilized.Sterilizationcanbeaccomplishedsterilealcoholwipes.Experiencedqueeninseminatorswilltellyouthatoneofthemostlikelysourcesoffailedinseminationisaresultofinfectionfromcontaminatedhooksandsyringes.
6. Syringe:Dependingonwhichtypeofsyringetheuserpreferswilldeterminethepropercleaningmethod.ManyuserspreferthelargerHarbostylesyringewhileotherspreferthesmallersyringethatinstallsdirectlyintothesyringeholderontheQueenStationtm.
Thesyringeshouldbecompletelydisassembledandwipedwithasterilealcoholwipe.Thesyringeshouldbereassembledandtheexteriorwipedclean.Thesyringeprovidedwithyourinstrumentisa1mldisposableunitandshouldnotbecleaned.
7. Lights:Theexteriorofthelightscanbewipedwiththealcoholcloth.
Section4Co2System
TheintegratedCo2systemisoneofthesignificantfeaturesoftheQueenStationtmandneedstobefullyunderstoodandmanaged.FailuretounderstandtheCo2systemwillbeasourceofproblemsfortheuser.Ifyouastheuserisusedtousinga100cubicfootindustrialcylinderyoumayhavedevelopedahabittonotbeconcernedabouttheamountofCo2availabletoyou.YouwillquicklybecomecognizantofCo2conservationwiththissystem.Itiscriticaltofollowthestepslistedheretobesuccessful.
1. CartridgeInstallation/Removal:Priortoinstallingorremovingthecartridgeshutoffallthreecontrolvalves.Useonly74gCo2cartridge.ItisrecommendedtouseONLY74gramcartridgesthatareintendedforbeveragedispensingsystems.DONOTUSECo2CARTRIDGESINTENDEDFORPAINTBALLGUNSORFISHTANKS.74gCo2cartridgesintendedforbeverageservicecontainfoodgradeCo2andwilldoyourqueensnolastingharm.AsidefromthequalityoftheCo2containedinthecartridge,thethreadsandsealsintheQueenStationtmregulatormaynotmatchothercartridges.Sparecylinderscanbeobtainedlocallyfrombrewerystores,on-linefrombrewerystoresordirectlyfromApisEngineering.
10
InstallingtheCo2cartridgeisstraightforward.Simplyscrewthecartridgeintothethreadsontheregulatorbody.ThereisasmallprojectioninsidetheCo2regulatorbodythatpiercesthesealoverthecartridge.Itisimportantthatthecartridgebescrewedoncompletelyandhandtightwithoutstoppingthethreadingmotion.Iftheuserinterruptsthethreadingmotionitispossibletocausethegastoescapebeforethecartridgeisfullyseatedagainsttheseal.Oncethecartridgeisinstalleditshouldnotberemoveduntilitisempty.IfthereisCo2gasremaininginthecartridgeanditisremovedthegaswillescapeandthecartridgewillcompletelyempty.Itcannotbesealedandreused.Thecylinder,ifremovedwithCo2gasremaining,willnotpresentadangerexceptthaticemayformontheexteriorofthecartridge.Ifyoupurchasedtheoptionaltravelcasethefoamlinerhasbeendesignedtoprotectthecylinderwithitremainingontheregulatorbody.
2. WaterFill:Withallthreecontrolvalvesclosedthebubblecountercanbefilledwithwater.Note:onlydistilledwatershouldbeusedinthebubblecounter.Distilledandde-ionizedwaterisacceptablebutnotrequired.Thebubblecounterisfilledbyremovingthesmallthumbscrewonthesideofthepolycarbonatetube.Includedwithyourinstrumentisa3mlblunttippedsyringe.Fillthissyringewithdistilledwaterandthenfillthebubblecounterthroughthethumbscrewhole.Fillthepolycarbonatetubetoapproximately1/8”belowthethumbscrewhole.ReplacethescrewmakingsurethesmallO-ringisstillonthescrew.Fromtime-to-timethebubblecounterwillneedrefilled.ThisisbecauseminuteamountsofthewaterbindtotheCo2andiscarriedoutofthesystem.Whentheuserneedsto“topoff”thesystemallvalvesneedtobeclosedandthesystemrechargedwithCo2asdescribedbelow.
3. PressureAdjustmentProcedure:InordertoeliminatewastingCo2andhenceprematuredepletionofCo2cartridgethefollowingproceduremustbeadheredto.
a. Checktomakesureallthreecontrolvalvesareclosed.Thevalveswillbeoffifthestep#1(above)wasfollowed.
b. AfterinstallationofCo2cartridgeturntheregulatorknobclockwisetoanindicatedpressureof5psiasshownonthedial.Atnotimeismorethan5psirequiredtoanesthetizequeens.Afteranyofthethreevalveshavebeenopenedtheuserneedstowait15secondsbeforeopeningthenextvalve.This15seconddelayallowsthepressuretostabilizewithinthesystem.Keepinmindyourgoalistoproducea3bubblepersecond(bps)flowrate.ThisisanextremelysmallvolumeofCo2gasandittakestimetofillthelines.ItshouldbenotedthatCo2isheavierthanairandthisworkstoyouradvantagewithinthe
11
systembecauseallairwillbedrivenoutofthelines.Bepatienthere.
c. Afterwaiting15secondsthenextvalvetoopenistheflowregulator.Thisisthebigblackknobwiththeredlockring.Theredlockringneedstobeliftedupinordertoadjusttheknob.Raisetheredringandopentheknob(turncounterclockwise)onefullturn.Pushtheredlockringbackdown.Wait15secondsagain.OpeningthisvalveallowstheCo2gastoflowthruthelinestothebubblecounter.Atthispointthebubblecounterispressurizedbutnobubblesareobservablebecausetheflowcontrolvalveontopofthebubblecounterisclosed.Bubblescanonlyappearoncethisvalveisopened.
d. Thissectionisthemostcriticaladjustment.Slowlyopenthevalveontopofthebubblecounterbyturningcounterclockwise.Ifthisvalveisopenedtoquicklythebuiltuppressureinthelineswilldriveallofthewateroutofthebubblecounterandintothelinesfeedingthequeenholder.AfterasmallopeningofthevalveWAITtoseeifanybubblesappear.Oncebubblesappearthevalveonthetopofthebubblecountercanbeadjustedforapproximately3bps.Atthispointyoumayrecallreadinginotherpublicationsthat8-10bpsisappropriateandinothersyoumayhavereadthatcontinuousCo2isrequired.Whythedifferences?Itsimplyamatterofbubblesize.Thebubblesgeneratedinthissystemflowthroughapreciseorificethatcontrolsbubblesizeand3bpsisadequate.
e. Ifafteropeningthevalveonthetopofthebubblecounterdoesn’tproduceanybubblesthenclosethisvalvecompletelyandgobacktotheflowcontrolvalveandliftuptheredringandopenthisvalveanother½turn.Pushtheredlockringdownandrepeatsection(d).
4. DrainingBubbleCounter:Occasionallyitmaybenecessarytodrainthesystem.a. ShippingorLongTermStorageofInstrument:Iftheuserneedstoshipthe
instrumentviaanymethodi.e.airtransportorgroundthebubblecountershouldbedrained.Additionally,iftheinstrumentistobestoredforlongperiodsitshouldbedrained.Thisisneededfortworeasons.1)Ifyoushipyourinstrumentitwilllikelybeturnedonitssideorupsidedownandthewaterwillleakout.And2)Longtermstorageandshipmentmayexposetheinstrumenttofreezingconditions.
b. DrainingtheBubbleCounter:Iftheinstrumentneedsdraineditishighlylikelythatyoualsoneedtodisassembleittoo.Inanyevent,todrainthesystemtheinstrumentneedstobedisassembled.Oncedisassembledremovethesmallthumbscrewinthesideofthebubblecounterandturntheinstrumentupsidedownandallowthewatertodrainoutofthethumbscrewhole.
12
5. TemporarilyShuttingOffCo2Flow
a. BetweenQueens:AftercompletingtheinseminationofaqueeniftheuserisgoingtoimmediatelyinseminateanotherqueennothingneedstobedonetoshutofftheCo2flow.If,however,thereisgoingtobesomelengthoftime(morethan3minutes)itisanadvantagetoshutdowntheCo2flow.TotemporarilystopCo2flowsimplyclosetheflowcontrolvalveontopofthebubblecounter.Asyouturnthevalvetrytorememberhowmanyturnsitrequiredtocompletelyclosethevalve.Thiswillmakeiteasiertorestoreflowifyouhaveanideaofwherethevalvewaspreviouslyset.Note:WhenyouneedtoopenthevalvetorestoreCo2flow,openthevalveslowlyandinsmallincrementswithapausebetweeneachadjustment.Insummary,forshorttermCo2flowstoppagesallofthevalvesdonotneedtobeclosedonlythevalveontopofthebubblecounter.
6. LongTermShuttingOffCo2Flowa. FinishedUsingtheInstrument:Uponcompletionofinseminationofqueensit
willbenecessarytoshutoffCo2flow.Thisisaccomplishedbyclosingallthreevalves.ClosethevalveontheCo2regulatorfirst.ThisisaccomplishedbyturningtheknobCounterclockwise.Oncethisvalveisclosedthepressuregaugewilldroptozero.Next,lifttheredringontheflowcontrolvalveandclosethevalvebyturningtheknobClockwise.Finally,closethevalveontopofthebubblecounterbyturningtheknobClockwise.
Section5InstallingQueeninQueenHolder:Includedwithyouinstrumentaretwoplastictubes.Oneofthetubesistaperedononeendandtheotherissquareonbothends.Thetaperedtubeisusedtoholdthequeenduringanesthetizationandinseminationanditisalsousedtotrapthequeenpriortoinsemination.Thetubewithoutthetaperedend,sometimesreferredtoasthe“back-up”tube,isusedtocausethequeentobackupintothetaperedtube.Onceyou’veselectedaqueenforinseminationplaceherinthesquarecuttube.Oncesheisinthetubeplacethesquareendofthetaperedtubeagainsttheothertubeattheendofthetubethathasthequeens’stingshowing.Bepatientandshewillbackupintothetaperedtube.Placethetubeonthequeenholderpostontheinstrument,whichshouldhaveCo2flowing,andshewillbeanesthetizedandreadyforinsemination.
13
Section6SecondaryAnesthetizationofQueens
Empiricalevidencehasshownthatsecondaryanesthetizationofqueenscausesthemtobeginlayingsoonerandforlongerperiods.Yourinstruments’accessorykitboxdoublesasthesecondarysystem.TheboxhasventholesinthelidandaCo2portonitsside.AlsoincludedisaconnectorhosethatistobeattachedtothequeenholderpostontheQueenStationtmandthesecondarybox.Thesecondaryanesthetizationboxwillholdsevenoftheso-calledCaliforniaMinicages.Theflowrateforthesecondaryboxismaintainedat3bps.Thisratewillfilltheboxandforcetheairoutthroughtheholesinthelid.
Figure9Photoshowingmethodofconnectinghoseforsecondaryanesthetizationofqueens.
14
Figure10Photoshowingsecondaryanesthetizationofqueenswithboxclosed.
Section7LightingSystem
TheQueenStationtmincludesabuiltinlightingsystemthatiscontrolledbyamicroprocessordimmersystem.Thissystemcompletelyeliminatesthecostlyandspacewastingindependentsystems.
1. Installation/Removal:Thelightsareeasilyinstalledbypressingthe3pinbaseintothesocket.Itdoesnotmatterwhichlightgoesinwhichsocket.Toremovethelightspressthetabonthefrontedgeofthebasemountedconnectorandpulluponthebaseofthelamp.Note,donotpullupontheflexibleshaft.
2. Alignment:Thealignmentofthelightsisentirelydecidedbytheuserasregardswheretheuseriscomfortablewiththeillumination.Therecommendedmethodisasshowninthephotos(figure5and6).Bycurlingthelightsaroundthemicroscopeknobsasshowitkeepsthelightsoutofthewaywhenadjustingthemicroscopeandthesyringeslide.
3. Adjustment:Thelights’intensityisadjustedusingthepotentiometerontherightsideofthebase.Turningtheknobcompletelycounterclockwiseturnsthelightsoff.Turningtheknobclockwiseincreasestheintensity.Notethatbothlightsmustbeinstalledonthebaseforproperoperation.
15
Section8Storage
1. PackinginTravelCase
Thefollowingfiguresshowtheproperpackingoftheinstrument.
Figure11Level1,Level1,bottomlayerincaseshowingbase,lights,extraCo2andinstalledCo2
16
Figure12Level2,showingelectriccordsandhosesstorageandsyringeslide
Figure13Level3showingmicroscopehead,microscopebeam,ventralanddorsalposts
17
Figure14Level4showingcamera,storagebox,microscopebeamandclamp
Section9Accessories
1. AccessoriesStorage/SecondaryAnesthetizationBox2. GlassCapillaryTubetip(5ea.)3. TygonConnectionHose4. PVCSecondaryHose(one18”x¼”idandone12”x1/8”id)5. ¼”x1/8”reducingcoupling6. 3mlbluntTipSyringe7. 1mlsyringeinseminationsyringe8. 3mlsyringewithblunttip9. Stingforceps10. VentralHook
Alsoincluded:274gCo2cartridges.
18
Section10ElectricalSchematic
19
Co2Schematic
Related Documents
