Simplify your life www.imv-technologies.com Easy to use Time saving Repeatable Safe EasyKit TM range of Flow Cytometry assays Bacterial concentration / Viability and acrosome integrity / Oxidation Molecule H / Oxidation Molecule D / Mitochondrial activity / Viability or concentration EasyCyte II Plus Ref. 022841 1 laser and 5 parameters (FSC + SSC + 3 colors), 96-well plate platform and 10 positions for tubes EasyCyte II Mini Ref. 022843 1 laser and 5 parameters (FSC + SSC + 3 colors), for tubes EasyClean Ref. 024760 Washing solution Easysoft Ref. 022173 Quantity of analysis 024708 EasyKit 1 Viability or concentration 480 024864 EasyKit 2 Mitochondrial activity 480 025157 EasyKit 3 Oxidation Molecule D 480 025158 EasyKit 4 Oxidation Molecule H 480 025293 EasyKit 5 Viability and acrosome integrity 480 025402 EasyKit 6 Bacterial concentration 240 022162 EasyBuffer A Buffer for clear media 023862 EasyBuffer B Buffer for eggyolk media 023862 EasyBuffer C Buffer for bacterial test 06/2013 – Non contractual pictures and specifications + 33 (0) 233 346 464 [email protected] www.imv-technologies.com Each EasyKit contains: 96 with lyophilized fluorochromes 1 working base 1 black plate lid
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Simplify your life
www.imv-technologies.com
Easy to useTime savingRepeatableSafe
EasyKit TM
range of Flow Cytometry assays
Bacterial concentration / Viability and acrosome integrity / Oxidation Molecule H /Oxidation Molecule D / Mitochondrial activity / Viability or concentration
EasyCyte II PlusRef. 0228411 laser and 5 parameters (FSC+ SSC + 3 colors), 96-wellplate platform and 10 positionsfor tubes
EasyCyte II MiniRef. 0228431 laser and 5 parameters (FSC+ SSC + 3 colors), for tubes
EasyClean Ref. 024760Washing solution
EasysoftRef. 022173
Quantity of analysis
024708 EasyKit 1 Viability or concentration 480
024864 EasyKit 2 Mitochondrial activity 480
025157 EasyKit 3 Oxidation Molecule D 480
025158 EasyKit 4 Oxidation Molecule H 480
025293 EasyKit 5 Viability and acrosome integrity 480
025402 EasyKit 6 Bacterial concentration 240
022162 EasyBuffer A Buffer for clear media
023862 EasyBuffer B Buffer for eggyolk media
023862 EasyBuffer C Buffer for bacterial test
06/2
013
– No
n co
ntra
ctua
l pic
ture
s an
d sp
ecifi
catio
ns
+ 33 (0) 233 346 [email protected]
Each EasyKit contains:
96 with lyophilized fluorochromes1 working base1 black plate lid
140306_4p_A5_Easykit_2014_Mise en page 1 18/03/14 11:29 Page1
Indicates percentage of viable spermatozoa orconcentration.
By using ready-to-use kits, we can optimizetechnician time and everybody is able to dothe protocols now. Discarding a completebottle of fluorochromes due to error isexpensive and thanks to the kit, we have nomore this problem.
(Dr xxx, xxx Insitute, Country)
Easy-to-useUse only the number of required wells
Working base tailored for all plate systems
Viability or concentration
Time-savingEliminate fluorochromes aliquoting,preparation and distribution of mix
RepeatableEliminates errors in mix preparation,
minimize technician effect
SafeReduce fluorochromes handling
No direct contact with liquid fluorochromes
EasyKit TM
rangeProduct description• Usable with any flow cytometer • 96 well-plates with breakable strips• Lyophilized fluorochromes• Validated with fresh and post thawed semen
diluted in egg-yolk based or egg-yolk free media• Intense fluorescence for easier population identification• Validated for bovine and swine semen
Analyzis membrane potential (polarized, depolarized)to show the integrity of the mitochondria.
Mitochondrial activity
Assesses the oxidation status of the sperm by measuring the reactive oxygenspecies: H2O2, HOCI, ONoo.
Oxidation Molecule D
This assay measures the intracellular level of Reactive Oxygen Species H2O2,HOCI, ONoo needed for sperm function but that are harmful at a high level.
Oxidation Molecule H
Monitor the integrity of the acrosome and of the membrane, simultaneously.
Viability or concentration
Live Dead
High potential Low potential
Stongly oxydized
Contamination produces alterations on spermquality. This kit counts number of alive anddead bacteria
• Bacterial contamination in doseseconomic loss for AI centers
• Reduced fertility (sperm quality impact)• Sanitary problem (disease transmission)
Bacterial concentration Premature acrosome reaction
Reduce viability
Reduce sperm motility
Two solutions to analyze bacterial contamination
Petri Dish (Agar plate) Flow cytometry
Result turnaround time
Results
Comment
3 days
Count only live bacteria**
Subjective concentration(around x FUC/ml)
20 minutes
Count live AND deadbacteria
Precise concentration (x bacteria/ml)
**a sample with a lot of dead bacteria will not be detected as positive contaminated with Petri Dish method but beforedying, bacteria could have a bad impact on sperm quality.
Snapshots of bull semen samples diluted in egg yolk medium
Low bacterial contamination
Medium bacterial contamination
High bacterial contamination
Remark:the flow cytometer will give you a totalconcentration of bacteria
140306_4p_A5_Easykit_2014_Mise en page 1 18/03/14 11:30 Page2
Related Documents
