AXE 9 - Chimie analytique Techniques couplées Quantitative proteomics: different approaches OBJECTIVES - Define the scope of quantitative approaches in proteomics - Be aware of the different quantification strategies - Be able to implement an experimental approach, from sample preparation to critical analysis of the data obtained AUDIENCE Technicians, engineers, researchers PRE-REQUIREMENT A first experience in proteomic analysis by mass spectrometry For example, to have taken the course "Caractérisation des protéines par spectrométrie de masse dans le contexte de la protéomique" (Ref. 20156, this catalog) or equivalent level TRAINING PROGRAMME Lectures (30 %) - Global approaches: . label-free quantification (DDA, DIA, pSMART) . metabolic labeling: SILAC, dynamic SILAC, pulsed-SILAC, other metabolic labeling strategies . chemical labeling: iTRAQ, TMT and derivatives, other labeling - Targeted approaches: SRM / MRM, PRM (relative or absolute) Practical training (50 %) - Sample preparation for TMT labeling and label-free quantification - LC-MS/MS acquisitions on the instruments (Orbitrap mass spectrometers) Trainees can bring two samples for educational purposes (contact trainers for practical details) Data analyses workshops (20 %) - Presentation of the main data analysis software in quantitative proteomics - Establishment of the analysis procedures according to the type of quantification desired (relative or absolute) - Evaluation by trainees of different software solutions EQUIPMENT Mass spectrometers: Orbitrap Fusion Tribrid, Orbitrap Q-Exactive Plus, MALDI TOF/TOF 4800+ Chromatography : nano-LC-Easy 1000, nano-LC-Easy 1200, RSLC U3000 Dionex The laboratory has autonomous computing resources (Alineos Cluster) and dedicated software suites (MASCOT Site License, Peaks, Progenesis Qi, Byonic, Pinnacle, Proteome Discoverer, Skyline Open Source License) which can be used depending on the needs during the training session. SPEAKERS J.-M. Camadro (researcher), S. Terrier, L. Lignières, V. Legros (engineers) and G. Lelandais (professor) Scientific and technical environment of the training course Institut Jacques Monod http://www.ijm.fr COURSE DIRECTOR Jean-Michel CAMADRO Directeur de recherche UMR 7592 LOCATION PARIS (75) ORGANISATION 4 days From 08:00 am to 05:00 pm From 5 to 8 attendees Training course in English Practical sessions in subgroups of 2 trainees with one speaker per subgroup TRAINING FEES 2100 Euros AT THE END OF THE TRAINING COURSE Satisfaction survey from trainees A certificate of training is delivered. COURSE DATE Ref. 20 159 : from Tuesday 24/03/20 to Friday 27/03/20 cnrs formation entreprises - Tél. : +33 (0)1 69 82 44 55 - Email : [email protected] - http://cnrsformation.cnrs.fr 192 Powered by TCPDF (www.tcpdf.org)