Application Note #1824322 Quantitative LC-MS/MS analysis of Sulfonamides in Honey using Advance UHPLC-EVOQ™ Elite LC-MS/MS system Abstract A simple, robust and effective LC-MS/MS method operated in multi- ple reaction monitoring (MRM) mode is developed for simultaneous estimation of “Sulfonamide Drugs” in food grade honey with easy and minimal sample preparation. In this experiment, two MRM tran- sitions were tracked for each targeted analyte, one for quantification and the other for qualification. The limit of quantification achieved for all Sulfonamides was 0.1 ppb with consistent ion ratio of quantifier to qualifier ions. The method was found to be linear and repeatable with regression (R) 2 of ≥ 0.996 and relative standard deviation of <12% for all nine Sulfonamides under study. Use of EVOQ TM Elite Triple Quad LC-MS/MS system offers high sensitivity, specificity and flexibility of the method and allows high sample throughput for screening and quantification of Sulfonamides in honey. Introduction Sulfonamide drugs are commonly used for the prevention & treat- ment of bacterial growth mainly because of their high and broad spectrum activity. In addition, they are also used to fight disease in honey bees. Residues of these Sulfonamide drugs are quite often found in honey samples and are a matter of great concern to con- sumers around the world due to their toxic and/or allergic reactions they induce in hypersensitive humans and potential transfer of drug-resistant bacteria’s from honey to humans. Because of these harmful effects, different countries have estab- lished MRLs (maximum residues limits) for these antibiotics in food of animal origin products. The European Union has set a MRPL (min- imum required performance level) for Sulfonamide’s at a level of 10μg/kg in honey 1 . There are several techniques available for deter- mination and quantification of these Sulfonamides; however, due to insufficient sensitivity and poor selectivity posed by very complex matrix, the targeted MRPL’s cannot be measured using those tech- niques. The use of LC-MS/MS has shown good selectivity and sen- sitivity to quantify the sulphonamides at the required MRPL levels in complex food matrices. This application note describes a quick, simple, sensitive & rugged method for simultaneous determination of Sulfadimethoxin (SMM), Sulfadiazin (SD), Sulfadoxin (SDM), Sulfamethazine (SM2), Sulfadim- idine (SDN), Sulfamerazine (SM1), Sulfathiazole (ST), Sulfamethoxy- pyridazin (SMP) and Sulfamethizole (SMZ) residues in honey using Bruker’s Advance UHPLC system hyphenated with EVOQ TM Elite Tri- ple Quad MS/MS system. The method was validated as per the cri- teria specified in European Union Commission Decision 2002/657/ EC1.
4
Embed
Quantitative LC-MS/MS analysis of Sulfonamides in Honey ... · Quantitative LC-MS/MS analysis of Sulfonamides in Honey using Advance UHPLC-EVOQ™ Elite LC-MS/MS ... Sulfadiazin (SD),
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Application Note #1824322Quantitative LC-MS/MS analysis of Sulfonamides in Honey using Advance UHPLC-EVOQ™ Elite LC-MS/MS system
AbstractA simple, robust and effective LC-MS/MS method operated in multi-
ple reaction monitoring (MRM) mode is developed for simultaneous
estimation of “Sulfonamide Drugs” in food grade honey with easy
and minimal sample preparation. In this experiment, two MRM tran-
sitions were tracked for each targeted analyte, one for quantifi cation
and the other for qualifi cation. The limit of quantifi cation achieved for
all Sulfonamides was 0.1 ppb with consistent ion ratio of quantifi er to
qualifi er ions. The method was found to be linear and repeatable with
regression (R)2 of ≥ 0.996 and relative standard deviation of <12% for
all nine Sulfonamides under study. Use of EVOQTM Elite Triple Quad
LC-MS/MS system offers high sensitivity, specifi city and fl exibility
of the method and allows high sample throughput for screening and
quantifi cation of Sulfonamides in honey.
Introduction Sulfonamide drugs are commonly used for the prevention & treat-
ment of bacterial growth mainly because of their high and broad
spectrum activity. In addition, they are also used to fi ght disease in
honey bees. Residues of these Sulfonamide drugs are quite often
found in honey samples and are a matter of great concern to con-
sumers around the world due to their toxic and/or allergic reactions
they induce in hypersensitive humans and potential transfer of
drug-resistant bacteria’s from honey to humans.
Because of these harmful effects, different countries have estab-
lished MRLs (maximum residues limits) for these antibiotics in food
of animal origin products. The European Union has set a MRPL (min-
imum required performance level) for Sulfonamide’s at a level of
10μg/kg in honey1. There are several techniques available for deter-
mination and quantifi cation of these Sulfonamides; however, due to
insuffi cient sensitivity and poor selectivity posed by very complex
matrix, the targeted MRPL’s cannot be measured using those tech-
niques. The use of LC-MS/MS has shown good selectivity and sen-
sitivity to quantify the sulphonamides at the required MRPL levels in
complex food matrices.
This application note describes a quick, simple, sensitive & rugged
method for simultaneous determination of Sulfadimethoxin (SMM),