Quantification of Post- Quantification of Post- transplantation transplantation Chimerism: Chimerism: Algorithm for Algorithm for Correcting Systematic Correcting Systematic Errors Errors Moshe Israeli Computational Biology Undergraduate Program, Department of Life Science, Bar- Ilan University Project Supervisor: Prof. D. Kristt, M.D. Laboratory of Immunogenetics and Histocompatibility/ Tissue Typing - Rabin Medical Center, Petach-Tikva.
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Quantification of Post- transplantation Chimerism: Algorithm for Correcting Systematic Errors Moshe Israeli Computational Biology Undergraduate Program,
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Quantification of Post-Quantification of Post-transplantation Chimerism: transplantation Chimerism:
Algorithm for Correcting Algorithm for Correcting Systematic ErrorsSystematic Errors
Moshe IsraeliComputational Biology Undergraduate Program, Department of Life Science, Bar-Ilan University
Project Supervisor: Prof. D. Kristt, M.D.
Laboratory of Immunogenetics and Histocompatibility/ Tissue Typing - Rabin Medical Center, Petach-Tikva.
Bone Marrow TransplantationBone Marrow Transplantation
Anecdotal Sources of Variability in Chimerism Testing
Conclusions and Conclusions and RecommendationsRecommendations
• Constant Total amount of DNA.• Marker quality - reliability constant, α.• Size Difference – • f(AiB)
)( SizeSize RDabs
gC
Identify informative loci
Formula for Quantitative Formula for Quantitative Mixed Chimerism Calculation:Mixed Chimerism Calculation:
n
TotalDD
RDabsgC
AiBf
Chimerism
n
i SizeSizeii
1
21 )]())(
()([
%
•Improve post-tx patient monitoring
•Increase laboratory reliability.
•Provide clinicians with accurate data.
•EFI, Israeli Transplant Society, ISHI.
•Future research:
•Blood.
•ADMO, ASHI.
AcknowledgmentsAcknowledgments• Project Supervisor: Prof. D. Kristt, M.D.
Department of Interdisciplinary Studies, Bar-Ilan University.And.Laboratory of Immunogenetics and Histocompatibility/ Tissue Typing - Rabin Medical Center, Petach-Tikva.
• Dr. Tirza Klein – Director of The Laboratory of Immunogenetics and Histocompatibility, RMC.
• Dr. Jerry Stein and Dr. Isaac Yaniv of the BMT Unit, Department of Pediatric Hemato-oncology, Schneider Childrens’ Medical Center.
The End
Chimerism MonitoringChimerism Monitoring• Function of graft• Prediction of negative events .