australian proteome analysis facility PTMs - Glycan Analysis APAF - AUSTRALIA’S PREMIER PROTEOMICS PROVIDER SINCE 1995 APAF’s mission is to assist the scientific community address their protein analysis needs. Australian Government support has enabled APAF to create a world-leading proteomics analysis facility containing the latest infrastructure for advanced protein analysis. Our strengths in comparative analysis, along with absolute protein quantitation, have increased our capabilities to deliver leading edge proteomic services. Glycan analysis explained Glycosylation is one of the most universal and structurally diverse post-translational modifications (PTM) with more than half of all mammalian proteins predicted to be glycosylated. The significance of correct glycosylation in various biological events such as protein secretion, stability and immunogenicity and the direct correlation of aberrant glycosylation with diseases like cancer and congenital disorders of glycosylation are well documented. Identification and characterisation of glycans together with their particular protein carriers is crucial for deciphering glycan functionality in normal and diseased conditions. APAF has a documented record of glycan analysis with leading biotechnology firms. APAF’s services: • Release of N-Linked glycans (using PNGase F) and O-Linked glycans (using beta-elimination) from purified proteins for comparative profiling using carbon LC/MS/MS • Glycan structural analysis using Exoglycosidase and MS/MS fragmentation • N-Linked glycopeptide analysis of lgG antibodies using reversed phase LC/MS/MS • Monosaccharide analysis of purified eukaryotic glycoproteins using HPAEC-PAD WE DELIVER LEADING EDGE PROTEOMIC SERVICES We provide services to: • Research Institutes • Universities • Pharmaceutical R & D • Biotech R & D Glycopeptides IgG Our expertise includes: 1. World class expertise in glycobiology 2. Leading edge mass spectrometry instrumentation 3. Established methods for glycan characterisation 4. Development of QC methods for recombinant protein/antibody batch control ENQUIRE NOW! E: [email protected] F: +61 2 9805 3101 skype. apafbdm T: +61 2 9805 3175 M: +61 416 250 739 APAF - Australian Proteome Analysis Facility Ltd. Level 1, 3 Innovation Rd Macquarie University Sydney NSW 2109 Australia www.apaf.com.au