PRRSV Diagnostics, PRRSV Diagnostics, specificity or specificity or sensitivity, that’s the sensitivity, that’s the question question Eric van Esch Eric van Esch Technical director Technical director BioChek BV, Reeuwijk, Netherlands. BioChek BV, Reeuwijk, Netherlands.
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PRRSV Diagnostics, specificity or sensitivity, that’s the question
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PRRSV Diagnostics, specificity or PRRSV Diagnostics, specificity or sensitivity, that’s the questionsensitivity, that’s the question
Eric van EschEric van Esch
Technical director Technical director BioChek BV, Reeuwijk, Netherlands.BioChek BV, Reeuwijk, Netherlands.
PRRSV diagnostics
• Definitions
• Reasons false positives or false negatives
• Setting cut off
• Conclusions
Diagnostics, definitions
• What has to be detected?
– Antigen• Different types• Different strains
– Antibodies• Different classes
Diagnostics, definitions
• Setting up an assay– Depending on what has to be detected
– Additional questions• Discrimination between field infected and vaccinated animals?• Discrimination between types / strains?• Detection limits?• Required sensitivity and specificity?• Regulations (national, OIE)?• ……
Diagnostics, definitions
• In what situation will the assay be used?
– Clinical diagnosis• Antigen present on farm, clinical cases from time to time
– Herd screening• Establishing herd status, clinical cases present or not
– Eradication• Elimination of agens from the farm
– Monitoring• Control of freedom of disease
– Vaccine control• Success of vaccination
Diagnostics, definitionsInfected
Yes No
TestResult
Positive a b a+b
Negative c d c+d
a+c b+d a+b+c+d
• Prevalence = a+c/a+b+c+d
• Sensitivity = a/a+c
• Specificity = d/b+d
• Predictive value positive = a/a+b
• Predictive value negative = d/c+d
Diagnostics, false positives and false negatives
• Selection of materials– ELISA antigen• Whole virus vs recombinants
– PCR primers• Type specific? General?
Diagnostics, false positives and false negatives
• Reasons of false negative results– Test itself– Time of sampling (too soon or too late)– (Very) low level of antibodies / antigen– Immunosuppression– Prozone, high dose Hook effect
Diagnostics, false positives and false negatives
• Reasons of false positive results– Test itself– Cross reaction with antibodies against related
antigen – Cross reactions by other factors– Bridging, non-specific adhesion (fat)
Diagnostics, setting cut off
Diagnostics, setting cut off
Diagnostics, setting cut off
Diagnostics, setting cut offClinical
diagnosisHerd
screeningEradication Monitoring
freedom of disease
Vaccine control
Prevalence 0 – 100% 0 – 100% 100 0% 0% 0 – 100%
Specificity + + + ++ +
Sensitivity ++ + ++ + +
Diagnostics, setting cut off
• Interpretation– Technical experimental samples– Diagnostic field samples