Laboratory Essentials SAVE 15% Isolation & Cloning SAVE 10% Protein Purification Save up to 15% Protein Analysis SAVE 12% Sample Preparation SAVE up to 25% Page 11 Page 6 Page 2 Page 3 Page 8 Prices valid until: 31 December 2018 Distributor GE Healthcare Protein Xtra 2018 Protein Workflow Simplified
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Protein Xtra 2018 - Bio-Strategy...2 Description Pack Size Code Protein Xtra Discount List Price (excl. GST) Sale Price (excl. GST) PERCOLL 250 ML GEHE17-0891-02 10% $254.00 $228.60
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Cell IsolationPercoll™ Density Gradient MediaPercoll™ is a low viscosity density gradient medium for preparation of cells, subcellular particles, and larger viruses. The low viscosity of the medium enables cell preparation on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 × g).
Ficoll-Paque™ PLUS Density Gradient MediaFicoll-Paque™ PLUS is a ready to use, sterile medium for isolation of lymphocytes in high yield from peripheral blood using a simple and rapid centrifugation procedure. It maintains the viability and a representative distribution of B and T lymphocytes.
Ficoll-Paque™ PREMIUM Density Gradient MediaFicoll-Paque™ PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.
• Manufactured under a Quality Management System certified to ISO 13485:2012 • Optimised for the isolation of mononuclear cells from human peripheral blood
PCR Amplificationillustra™ PuReTaq Ready-To-Go™ PCR BeadsFreeze-dried PCR reagents mix in a bead format
• Long-term ambient-temperature stable: no freezer space required • Save time: simply add template DNA solution and primers, and cycle • More reproducible results: less risk of pipetting errors and contamination
DNA Polymerisation Mix dNTP Set (20 mM each A, C, G, T) • Free from DNase, RNase, and nicking enzyme activity • Greater than 99% triphosphate purity (by HPLC) for optimum performance and
consistency • Buffer free and ready-to-use solutions in multiple formats
PCR Purificationillustra™ ExoProStar™ExoProStar™ 1-Step PCR and Sequence Reaction Clean-Up reagent provides a simple, single-step method for removing unincorporated primers and nucleotides prior to downstream analysis. illustra™ Exonuclease I and Alkaline Phosphatase enzymes are combined in a single, stable mix requiring just one pipetting step to set up the clean-up reaction.
• Two separate tubes for maximum flexibility• Fast 15 min protocol, different reaction sizes• Need a one-tube option? Choose ExoProStar™ S
illustra™ GFX PCR DNA and Gel Band Purification KitIsolation and concentration of DNA fragments from PCR mixtures, agarose gel bands, and enzyme digests in under 10 min.
• Flexible elution volume for different DNA concentrations • DNA for use in sequencing, PCR, labeling, digestion, and cloning
Nucleic Acid Purificationillustra™ NAP ColumnsDisposable columns prepacked with Sephadex™ G-25 DNA Grade which require only gravity to run. For rapid and efficient purification of DNA and oligonucleotides 10-mers in less than 15 minutes.
• Available in three sizes depending on sample volume: 0.5ml, 1ml or 2.5ml • Useful for small-scale purification, desalting and buffer exchange
Collection and StorageDNA Sample Collection and StorageFTA™ cards and FTA™ Elute cards utilise patented Whatman™ technology that simplifies collection, transportation, and storage of biological samples and processing of nucleic acids at room temperature. Simply apply your sample to the card, which contains chemicals that lyse cells, denature proteins, and protect nucleic acids from nucleases. The released nucleic acids are entrapped and preserved in the fibres of the matrix.
Analytical Sample Preparation1 Chr Chromatography PaperWhatman™ cellulose chromatography papers are used worldwide for chromatography. Produced from the highest quality cotton linters with no additives, the papers are of the purity, high quality, and consistency needed. The papers are manufactured and tested specifically for chromatographic techniques and undergo rigorous quality control to ensure uniformity.
1 Chr has a smooth surface, 0.18mm thick with a linear flow rate (water) of 130mm/30min. Good resolution for general analytical separations.
GF/F Glass Microfibre FilterManufactured from pure borosilicate glass. GF/F offers very fine particle retention and good loading capacity and flow rate.
• 0.7μm nominal particle retention • Served as the basis filter for EPA standard for the toxicity characteristic leaching procedure
Whatman™ Uniflo™ Syringe FiltersNow extended to include sterile 13mm and 25mm syringe filters.
Whatman™ Uniflo™ Syringe Filters are disposable filter units designed to provide clean filtrate from small volumes up to 100ml. They are available in a variety of membrane choices with a polypropylene overmold housing. Whatman™ Uniflo™ syringe filters are available with:
• 13 or 25mm diameters • 0.2μm, 0.22μm or 0.45μm pore sizes • Sterile or non-sterile options • Individual printing on the filter for easy identification • Bench top space saving packaging
Puradisc™ FP 30 Sterile Syringe FiltersThe FP 30 filter range contains a low protein binding cellulose acetate membrane, for sterile filtration of cell, tissue culture media and samples containing proteins. Available in different pore sizes.
SPARTAN™ HPLC Certified Syringe FiltersReproducible filtration for ultra-high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) sample preparation. SPARTAN™ filters can also be used in capillary electrophoresis, protein filtration, UV/VIS analysis, and any application requiring a chemically resistant, low proteinbinding, hydrophilic membrane in a polycarbonate housing.
• Hydrophilic regenerated cellulose membrane exhibits low protein binding • Excellent chemical resistance to common aqueous and organic solvents • Tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254nm
with water, methanol, and acetonitrile • Documented batch-to-batch quality and consistency, ensuring reproducible results
ReZist™ Syringe and Venting FiltersSpecifically designed to be resistant to organic solvents. Highly suitable for the clarification of aggressive organic solvents. ReZist™ 30mm filters can also be used as a venting filter for small vessels.
Mini-UniPrep™ Syringeless FiltersProvide a faster and easier way to remove particulates from samples being prepared for (U)HPLC. All-in-one device filtration process allows samples to be processed in one third the time. G2 glass version also available. Compatible with most major autosamplers.
High Resolution SEC ColumnsHigh-resolution columns including the new Superdex™ 200 Increase, Superose™ 6 Increase, and Superdex™ 75 Increase columns for polishing and characterisation of biomolecules.
Protein Purification
Vivaspin™Vivaspin™ spin columns are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration.
Sephadex™ G-25 in PD-10 Desalting Columns PD-10 Desalting Columns contain Sephadex™ G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
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Description Pack Size
Code Protein Xtra Discount
List Price (excl. GST)
Sale Price (excl. GST)
AMERSHAM™ ECL START, WESTERN BLOTTING DETECTION REAGENTS, (2*200ML)
1 KIT GEHERPN3244 12% $243.60 $214.37
AMERSHAM™ ECL START, WESTERN BLOTTING DETECTION REAGENTS, (2*100ML)
1 KIT GEHERPN3243 12% $152.30 $134.02
AMERSHAM™ ECL PRIME, WESTERN BLOTTING DETECTION REAGENT FOR 1000CM2
1 KIT GEHERPN2232 12% $316.00 $278.08
AMERSHAM™ ECL SELECT, WESTERN BLOTTING DETECTION REAGENT FOR 1000CM2
Western Blotting DetectionECL™ Western Blotting Detection Reagents:ECL™ based on horseradish peroxidase (HRP)-conjugated secondary antibodies has become the most commonly used detection method for Western blotting. It is a sensitive detection method, where the light emission is proportional to protein quantity. Minute quantities of proteins can be detected and quantitated.
• Longer shelf life: 18 month shelf life on ECL™ Select and Prime products
• Stability: ECL™ products are stable and stored at room temp
Amersham™ ECL™ Western Blotting Detection Reagents
16 *Minimum quantity for Special Price
Western Blotting DetectionECL Western blotting detection reagents: ECL based on horseradish peroxidase (HRP)-conjugated secondary antibodies has become the most commonly used detection method for Western blotting . It is a sensitive detection method, where the light emission is proportional to protein quantity . Minute quantities of proteins can be detected and quantitated
Longer shelf life: 18 month shelf life on ECL Select and Prime products
Stability: ECL products are stable and stored at room temp
Amersham ECL
For reliable detection of high to medium abundant proteins
Chemiluminescence
Amersham Protran
Amersham Hyperfilm (X-ray film) or ImageQuant LAS 500
< 2 h
• Routine protein detection
• Protein confirmation
• Overexpressed proteins
• Tagged proteins
Amersham ECL Prime
For quantitative analysis of high to low abundant proteins
Chemiluminescence
Amersham Hybond™ PAmersham Protran
Amersham Imager 600 series X-ray film is also an option
< 24 h
• Protein quantitation
• Protein confirmation
• Protein regulation and expression analysis
Amersham ECL Select™
For quantitative analysis of medium to very low abundant proteins
Chemiluminescence
Amersham Hybond PAmersham Protran
Amersham Imager 600 seriesX-ray film is also an option
< 2 h
• Protein quantitation
• Protein confirmation
• Protein regulation and expression analysis
Amersham ECL Plex™
For multiplex protein analysis and quantitation of high to very low protein levels
Fluorescence
Amersham Hybond LFPAmersham Protran Premium
Typhoon™ FLA seriesAmersham Imager 600RGB
> 3 months
• Multiplex analysis
• Normalization to housekeeping protein
• Analysis of post translational modifications and isoforms
Amersham ECL start
Chemiluminescence detection reagent for detection of high to medium abundant proteins
Chemiluminescence
Amersham Protran
Amersham Hyperfilm™ (X-ray film) or ImageQuant™ LAS 500
< 3 h
• Routine protein detection
• High expressed proteins e g house keeping proteins
• Tagged proteins
Product
Detection type
Optimal membrane
Recommended detection
Signal duration
Typical applications
Typical results
Amersham™ ECL™ Western blotting detection reagents
WB ERK ½; LOD: 20 ng; DR: 2.4
20 ng5 µg
WB ERK ½; LOD: 5 ng; DR: 2.4
5 ng1.2 µg
WB ERK ½ (Cy5 on FLA 9500); LOD 10 ng; DR: 3
5 ng10 µg
WB ERK ½; LOD: 40 ng; DR: 2.1
40 ng5 µg
WB ERK ½: LOD 320 ng; DR
320 ng10 µg
gelifesciences.com
Amersham™ ECL™ Western blotting detection reagents
20% OFF!
Description Pack Size Product Code *Min Qty Total List Price for Bundle
Total Special Price for Bundle
20% off!
Amersham™ ECL Start, Western Blotting detection reagents, (2 × 200ml)
1 x 1 kit GEHERPN3244 1 243 .60 —
Amersham™ ECL Start, Western Blotting detection reagents, (2 × 100ml)
1 x 1 kit GEHERPN3243 2 304 .60 243 .68
Amersham™ ECL Prime, Western Blotting detection reagent for 1000cm2
1 x 1 kit GEHERPN2232 2 632 .00 505 .60
Amersham™ ECL Select, Western Blotting detection reagent for 1000cm2
1 x 1 kit GEHERPN2235 2 788 .00 630 .40
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Analysis
Save 12%
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Description Pack Size Code Protein Xtra Discount
List Price (excl. GST)
Sale Price (excl. GST)
WESTERN BLOTTING MEMBRANESBLOTTING MEMBRANES, AMERSHAM™ PROTRAN™ 0.2ΜM NC, 300MM×4M
Western Blotting MembranesAmersham™ Western Blotting MembranesGE Healthcare Life Sciences offers a broad selection of nitrocellulose (NC) and polyvinylidine difluoride (PVDF) Western blotting membranes, with pore sizes ranges to suit your application requirements.
• Optimised for chemiluminescent and fluorescent detection • Excellent protein binding capacity over a wide size range • New larger pack sizes reduce your price per blot by up to 30%
Amersham™ Protran™NC membranes with high affinity for proteins, great blocking ability, and compatibility with a range of detection methods.
Amersham™ Protran™ SupportedNC membranes supported by an inert polyester nonwoven material, giving the membranes exceptional handling characteristics that allows for stripping and reprobing of the membrane.
Amersham™ Hybond™ PPVDF membranes with low background and high sensitivity and resolution, for all labeling and detection systems, but particularly recommended for chemiluminescence detection.
Amersham™ Hybond™ LFPPVDF membranes specifically optimised for fluorescence detection methods.
Amersham™ Molecular Weight MarkersAmersham™ ECL Full-Range Rainbow Molecular Weight Markers • Ready-to-load markers with sharp, intense bands on gels and blots enable
monitoring of electrophoresis progress and convenient confirmation of transfer to blotting membranes
• Comprised of 10 individually coloured proteins in the molecular weight range of 12,000 to 225,000.
• Each 250µl pack size is sufficient for use with 50 mini-gels (10x8cm) or 25 large gels (20x20cm)
Amersham™ ECL Secondary AntibodyHighly species-specific HRP-conjugated antibodies optimised for use with Amersham™ ECL Western blotting detection reagents.
Whatman™ Blotting PaperWhatman™ 3mm Chr Blotting Papers • Pure cellulose produced entirely from the highest quality cotton linters with no
additives of any kind • Ensures that no contamination will occur during the transfer step
• Whatman™ 3mm Chr considered the industry standard for blotting procedures
Save 12%
Analysis
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NEW WHATMAN™ CATALOGUE NOW AVAILABLELaboratory Filtration Guide - request your copy today!
The new Whatman™ Laboratory Filtration Guide includes all the products to get the results you want in your work! Whether you’re in the Food and Beverage, Pharmaceutical, Environmental monitoring or analytical testing markets, this guide has all your filtration needs.
Contact our Customer Service team or your local Bio-Strategy Sales Representative today!
Benchkote™ Laboratory Surface ProtectorWhatman™ Benchkote™ is an absorbent lab paper that protects surfaces against hazardous spills. After use, the bench protector sheet is incinerated or disposed of according to local regulations.
Benchkote™ Plus is a thicker, more absorbent paper for demanding or hazardous applications. It can absorb more than 0.75 liters of water per square meter.
Grade 556 DrypadsAbsorbent paper strips for the removal of excess liquids, e.g., from microscope specimens.
Grade 2122 Weighing PaperReliably allows samples to be transferred to scales without adding unwanted substances that could impact analytical results.
Whatman™ Lens Cleaning TissueHigh-quality lens cleaning tissue, which is chemically pure and free from silicones and other additives.
Contact us for more information and to place your orders
Prices & discounts in this brochure may be subject to change. Promotions are not valid with any other special offer or discounts and cannot be applied in retrospect. All offers subject to availability and while stocks last. All prices are in AUD excluding GST and local shipping & delivery charges. Promotion code (if stated) must be referenced on your purchase order to receive offer prices.
*The Polymerase Chain Reaction (PCR) is covered by patents owned by Roche Molecular Systems and F Hoffmann-La Roche Ltd. A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such as GE Healthcare and affiliates when used in conjunction with an authorised thermal cycler. Phi 29 DNA polymerase and its use for DNA synthesis is covered by US patent numbers 5,854,033.
Ready to Go RT-PCR Beads: Use of this product is covered by applicable US patents and corresponding patent claims outside the US held by Roche. The purchase of this product includes a limited, non-transferable immunity from suit under the patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Additional rights for use in non-diagnostic field require a license from Life Technologies. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting Out-licensing at Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, CA 92008; [email protected]