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Protein Synthesis $100 $200 $300 $400 $500 $100 $ 100 $100 $100 $200 $200 $200 $200 $300 $300 $300 $300 $400 $400 $400 $400 $500 $500 $500 $500 Central Dogma Basics Transcripti on RNA Mutations FINAL ROUND Translation
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Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Dec 18, 2015

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Page 1: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Protein Synthesis

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Central Dogma Basics Transcription RNA Mutations

FINAL ROUND

Translation

Page 2: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics:

$100 Question

This is the place where transcription takes place in eukaryotic cells

BACK TO GAME

ANSWER

Page 3: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$100 Answer

BACK TO GAME

Nucleus

Page 4: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$200 Question

BACK TO GAME

ANSWER

These are the parts of mRNA that do not code for protein

Page 5: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics:

$200 Answer

BACK TO GAME

introns

Page 6: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$300 Question

BACK TO GAME

ANSWER

This is the site where translation takes place

Page 7: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$300 Answer

BACK TO GAME

Ribosomes (in cytoplasm or on Rough ER)

Page 8: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics:

$400 Question

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ANSWER

This is a sequence of DNA that codes for protein(s)

Page 9: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$400 Answer

BACK TO GAME

Gene

Page 10: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$500 Question

BACK TO GAME

ANSWER

This is the central dogma

Page 11: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Central Dogma Basics :

$500 Answer

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DNA RNA Protein

Page 12: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription:

$100 Question

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ANSWER

This is the enzyme that synthesizes mRNA

Page 13: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$100 Answer

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RNA polymerase

Page 14: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$200 Question

BACK TO GAME

ANSWER

This is the region of DNA where RNA polymerase binds

Page 15: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$200 Answer

BACK TO GAME

Promotor

Page 16: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$300 Question

BACK TO GAME

ANSWER

This is the portion of DNA that signals transcription to stop

Page 17: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription:

$300 Answer

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Terminator

Page 18: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$400 Question

BACK TO GAME

ANSWER

This is the process takes place after transcription in which mRNA is edited

Page 19: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$400 Answer

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mRNA splicing

Page 20: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$500 Question

How does mRNA exit the nucleus

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ANSWER

Page 21: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Transcription :

$500 Answer

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Through nuclear pores

Page 22: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation:

$100 Question

BACK TO GAME

ANSWER

This is the start codon

Page 23: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation:

$100 Answer

BACK TO GAME

AUG

Page 24: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$200 Question

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ANSWER

This is the site on the ribosomes in which polypeptide chain grows

Page 25: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$200 Answer

BACK TO GAME

P site

Page 26: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$300 Question

BACK TO GAME

ANSWER

These are the type of bonds that exist between amino acids

Page 27: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$300 Answer

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peptide bonds

Page 28: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$400 Question

BACK TO GAME

ANSWER

This is the site on the ribosome in which the next tRNA enters the ribosome

Page 29: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$400 Answer

BACK TO GAME

A site

Page 30: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation:500

BACK TO GAME

ANSWER

Describe initiation of translation

Page 31: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :500

BACK TO GAME

mRNA finds a ribosome and binds to it; the start codon (AUG) codes for a tRNA carrying the amino acid methionine; this tRNA enters the ribosome at the P site and translation begins

Page 32: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA:

$100 Question

BACK TO GAME

ANSWER

This type of RNA contains codons

Page 33: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA:

$100 Answer

BACK TO GAME

mRNA

Page 34: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA:

$200 Question

BACK TO GAME

ANSWER

This type of RNA contains anti-codons

Page 35: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Translation :

$200 Answer

BACK TO GAME

tRNA

Page 36: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA :

$300 Question

BACK TO GAME

ANSWER

This type of RNA carries amino acids

Page 37: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA: $300 Answer

BACK TO GAME

tRNA

Page 38: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA:

$400 Question

BACK TO GAME

ANSWER

RNA is different from DNA because…..

Page 39: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA:

$400 Answer

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Ribose sugar; Uracil instead of Thymine; single stranded

Page 40: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA: $500 Question

BACK TO GAME

ANSWER

These are the types of RNA involved in translation

Page 41: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

RNA: $500 Answer

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mRNA, tRNA, rRNA

Page 42: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutations:

$100 Question

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ANSWER

This type of mutation codes for the same amino acid therefore there is no effect on the protein

Page 43: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation:

$100 Answer

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Silent mutation

Page 44: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation :

$200 Question

BACK TO GAME

ANSWER

This type of mutation involves a substitution of a nucleotide that results in a stop codon reached to soon therefore the polypeptide chain is not complete and the protein is nonfunctional

Page 45: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation : $200 Answer

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Nonsense mutation

Page 46: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation :

$300 Question

BACK TO GAME

ANSWER

Insertion or deletion of a nucleotide will result in this type of mutation

Page 47: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation : $300 Answer

BACK TO GAME

Frameshift

Page 48: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation:

$400 Question

BACK TO GAME

ANSWER

This type of mutation is a substitution which results in a different amino acid

Page 49: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation :

$400 Answer

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Missense mutation

Page 50: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation :

$500 Question

BACK TO GAME

ANSWER

Why can one difference in amino acid sequence be harmful?

Page 51: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

Mutation :

$500 Answer

BACK TO GAME

The protein shape can be altered by just one change in amino acid; if the shape is incorrect the function of the protein is incorrect

Page 52: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

FINAL ROUND Question

BACK TO GAME

ANSWER

Transcribe and Translate the following gene….

5’ TTTCAGCTACAAAGAGTAGATT 3’

Page 53: Protein Synthesis $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Central Dogma Basics Transcription RNA Mutations FINAL ROUND Translation.

FINAL ROUND Question

BACK TO GAME

ANSWER

DNA= 5’TTTCAGCTACAAAGAGTAGATT 3’

mRNA=3’AAAGUCAUGUUUCUCAUCUAA 5’

Amino acids= (start)met- phe- leu- iso- stop


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