Protecting the Crops with Nature’s Cure

Slide 1

Protecting the Crops with Natures Cure

1Nature's Cure- Purely Organic SA

Introduction

Natures Cure Background. Who uses Natures Cure? Crops and Natures Cure. Benefits of Natures Cure. Mechanisms of Action. Application and Procedure. Experiments: -Papaya: Delay of Ripening. -Grapes: Preventing and Curing Frost Damage. Physical and Chemical Properties. Stability and Reactivity. Toxicological Information. Ecological Information. Disposal Considerations. First Aid Measures. Conclusions. Contacts.

2Nature's Cure- Purely Organic SANatures Cure Background

Made from the most abundant nitrogen- bearing organic compound found in nature.

Enhance plant growth while boosting natural defenses.

Processed from marine byproducts (Shellfish waste from food processing ).

Chitosan

Known for been the second most prevalent naturally occurring long chain polymer.Triggers plant defenses due to its similarity to cell walls of fungi. Produces an ability to aggregate soil particles while reducing water inputs. Increases soil water retention capability during drought conditions.

3Nature's Cure- Purely Organic SA3Natures Cure

This is a unique product that enhance growth, increases plant vigor, stimulates nutrient uptake, increases shelf-life and protects plant from frost damage.

Active IngredientsChitosan & Yucca Plant Extract....8%Inert Ingredients..92%

It is a tan to brown viscous liquid with a slight odor.

Edible coating to maintain quality and extend shelf-life of fresh fruits and vegetables.

Prevent microbial storage (extremely important to perishable horticultural commodities).

It is a concentrated foliar application material to be used to enhance plant defenses and improve soil moisture retention.

Store at room temperature and out of direct sunlight.

4Nature's Cure- Purely Organic SAThis product is Appropriate for:

Organic Farmers wanting to enhance plant growth while boosting natural defenses. Biological Farmers wanting a reliable edible coating for fruits and vegetable that can prevent color changes, decay and ripening of climacteric fruits.

Conventional Farmers wanting to extend shelf-life, marketability and prevent lost of crops due to climate changes or normal morphology cycle.

Crops and Natures Cure

This product is essentially used with climacteric fruits and vegetables.

5Nature's Cure- Purely Organic SANote: See attachments for MSDS information and experiments with results from this product.

5Benefits of Natures Cure

Prevent and cure frost damage. Enhance growth and stimulates nutrient intake -Greatly increased root hair density. -Enhances the ability to capture all nutrients. Delay ripening of the climacteric fruits. Delay color changes. Edible natural defense coating. Reduce weight lost of plants pre and post harvest period. Extend shelf-life and marketability. Maintain texture of fruits and vegetable under hard climatic conditions Reduce decay of plants. It is an Organic product: - Environmentally friendly. - Simple and reliable treatment to use. Extension: storage can be up to 5 weeks facilitating the export of fruits and vegetables. Longer shelf-life make fruits and vegetables more competitive in the worlds market. Chitosan increases photosynthesis.

6Nature's Cure- Purely Organic SA

Natures Cure increases germination, sprouting and overall vigor of the plant.

Improves profitability : -Increase yields by enhancing the growth and boosting self of the plant.

Prevents fruits and vegetables from decaying.

Provide a protective shield during climates changes that can cause the death and shrinkage of the crop by the deficiency of nutrients .

Provides a back-up plan for growers that face climate emergency situations.

Manage fruits and vegetables growth thru the season.

7Nature's Cure- Purely Organic SABenefits of Natures Cure Mechanism

Preparing chitin and chitosan process (shellfish waste from food processing shrimp, lobster, crab, and squid). Decalcification in dil. Aqueous HCL solution (3%- 5% HCl w/v at room temperature.Deproteination on dil. aqueous NaOH solution (3%- 5% w/v NaOH 80o C to 900O C for a few hrs or room temperature over night. 8Nature's Cure- Purely Organic SA8Decolarization in 0.5% KMnO4 aqueous and oxalic acid aqueous or sunshine.

Deacetylation in hot concentrated NaOH solution 40%-50% w/v NaOH at 90o C to 120o C for 4-5 hrs. 9Nature's Cure- Purely Organic SA

After all these processes the formation of Chitosan takes place. Once You have created Chitosan, Yucca plant extract are addedand Natures Cure is formed.

Yucca plant extract.Chitosan & Yucca Plant Extract 8%Inert Ingredients ...92%Natures Cure 10Nature's Cure- Purely Organic SAApplication and Procedure

DIRECTIONS FOR USE:Always shake, stir, or agitate before use:

Always add product last to spray tank containing require amount of water. Mix: Pre harvest flower: 32 oz per acre. Post-harvest dipping solution: 1 oz in 1 gal of water.

Apply: Agricultural application: 32 oz per acre T&O application: 32 oz per acre

Application:

Via overhead irrigation in enough water to take the product into the foliar area. Via any irrigation system (micro-sprinkles, drip irrigation, trickle tape).Via liquid injection from a tine that cuts into the root zone.Via boom spray (Use very coarse or streaming nozzles).11Nature's Cure- Purely Organic SAExperiments and Trials

Papaya

Problems within the Product -Water loss -Early decay / ripening-Accelerated softening-Shrinkage/decline in texture-Investment lost -Low ProductionPost-Harvest Diseases- Anthracnose and chocolate spot, -Colletotrichum gloeosporioides (fruits, petioles)-Phytophthora, Phytophthora palmivora (fruit, stem, roots)-Powdery mildew, Oidium caricae (leaves)-Black spot, Cercospora papayae (fruit)-Damping off, Pythium, Phytophthora, Rhizoctonia (seedlings)-Wet rot, Phomopsis sp. (fruit)-Dry rot, Mycosphaerella sp. (fruit)-Watery fruit rot, Rhizopus stolonifer-Stem-end rot, -Botryodiplodia theobromae, Mycosphaerella sp.-Rhizopus stolonifer, Phomopsis sp. (mature fruit)-Papaya ring spot virus (formerly referred to as papaya mosaic)-Reniform nematodes, Rotylenchulus reniformis-Root-knot nematodes, Meloidogyne spp.

Insects -Stevens leafhopper, -Empoasca stevensi-Mediterranean fruit fly, Ceratitis capitata-Melon fly, Bactrocera cucurbitae-Oriental fruit fly, B. dorsalis

External Factors -Soil -Temperature Changes-Lack of nutrients -Water retention

Chemical Treatments Hot water immersion Storage at Low Temperatures12Nature's Cure- Purely Organic SACurrent Solutions Most prevalent problems that growers face:

13Nature's Cure- Purely Organic SA(A) Sunken anthracnose lesion caused byColletotrichum gloeosporioides. (B) Cross section of anthracnose lesion showinggrayish white discoloration of papaya flesh. Firm callose tissue forms at the border of the soft, semicircular lesion. (C) Chocolate spot lesions ranging from minute superficialspots (left) to large sunken lesions with water-soaked margins (center). (D) Cross section of chocolate spot lesions showing limited penetration into fruit parenchyma.(E) Mycosphaerella lesion with light brown, translucent margin. (F) Cross section of Mycosphaerella lesion showing a layer of firm, black tissue below the infection site.(G) Soft, translucent Phomopsis lesion with black pycnidia at center. (H) Cross section ofrapidly expanding Phomopsis lesion showing progress of decay into the seed cavity.Common Surface Rots of Papaya Fruit: Diseases that affect papaya:

14Nature's Cure- Purely Organic SA Sunken anthracnose lesion caused by Colletotrichum gloeosporioides is one of the major problems that growers face within the papaya production. Once the fruit is showing percentages of C. gloeosporioides colonizing ( >45%) the product has to be disposed. In 2012- millions were lost within the papaya production due to this situation.

Recovery by Farmers:Growers recovered some of the investment; however, heavy chemical treatments were added to papaya which increased farmers costs sustainably.

Results/ Problems with papaya:Even though growers recuperated SOME of the production the Papaya was affected.-Weight lost. -Los of exportation contracts. - Product remained local. -Dramatic color change. -Expensive product . -Shrinkage structure.-Changes in flavor, texture and firmness.Papaya Background- 2012

15Nature's Cure- Purely Organic SA We applied 5 different concentration of Natures Cure to see how the process of ripening will be delayed- the results were outstanding. Procedure:We selected the papayas for testing. A papaya control was prepared (no Natures cure solution on it).We chose 5 concentration of natures cure. [0.5%,1.0%, 1.5%,2.o%]Storage: root temperature and 120 C temperature.

16Nature's Cure- Purely Organic SAResults Under the MicroscopeAfter completing the experiment we took some samples from papaya.

We observed Anthracnose lesion caused by Colletotrichum gloeosporioides and how it was evolving under the different concentrations.

The results were very direct as we increased the concentration of Natures Cure in papaya. The Colletotrichum gloeosporioides did shrink and decreased in radius compared to the papaya that was not treated with Natures cure.

We clearly can observe that Colletotrichum gloeosporioides was present and abundant in the papaya that was not treated with Natures Cure. A healthy C. gloeosporioides in papaya shows a softness in structure, a color change, a ripening and modified the taste.16

17Nature's Cure- Purely Organic SANo Natures Cure 1.5% of Natures Cure ConcentrationResults : Disease Incidence/ Storage Duration 100%T1T4T1T2T3T4T5Contamination No Natures Cure 0%Contamination with Natures Cure

18Nature's Cure- Purely Organic SA Our control (No treatment with Natures Cure) showed a firm texture within the first two weeks.After the second week a considerable decline in texture, color and firmness was observed. While we observed this decline in firmness the storage time also decreased. Results: Firmness / Storage Duration Using the same procedure at room temperature: We saw the same pattern where the papaya that was not treated with Natures Cure showed an immediate change within 10 days after it was harvested.

However all the papaya that was treated with natures cure showed a healthier look, longer storage duration and greater firmness in texture. Room Temperature

19Nature's Cure- Purely Organic SASecond Experiment: Frost Damage on Grapes For a farmer it is very devastating tosee results as the ones show in this image. In here, the vines from Gallo vineyards were affected by frost damage. When a particular situation like this happen the results are very predictable.Problems:

Lost of production. Poor yield. Shrinkage in the product. Poor quality of the product.Higher investment in chemical treatments or costly equipments.

No Back-up Plan for Emergency SituationsNo Natures Cure application total lost of vineyards

20Nature's Cure- Purely Organic SAAfter these results we decided to make two different experiments:

Grapes Treated with Half Rate of Natures Cure. Grapes Treated with Full Rate of Natures Cure.

This imagine shows the vines that were treated with Half Rate of Natures Cure we can see in the leaf that there is an immediate recovery and there is only a minor level of frost damage in comparison to the vines that were not treated.

Only HALF RATE of Natures Cure was used on vines that were exposed to 320 F (0 0 C).

Under other conditions the fate of these vines would have been lost. Making the growers lost all the investment made on those vines.

The growers would have lost their entire investment,time, man power, and profit. Minor level of Frost DamageRecovery: Healthy Vines

21Nature's Cure- Purely Organic SAFull Rate Natures Cure After applying our product, it is very evident how these vines showed no frost damage.

Vines are growing healthy since Natures Cure helped to boost their defense mechanism, so they will be able to survive under irregular conditions like:

-Changes in climate temperatures-Deficiency of Nutrients Natures Cure give growers a back-up plan that will secure the protection of crops during emergency situations like the one Gallo vineyards faced. 21VAPOR DENSITY: No Data EVAPORATION RATE (n-BuAc=1): No DataSPECIFIC GRAVITY @ 20C: No Data SOLUBILITY IN WATER: SolubleVAPOR PRESSURE, mm Hg @ 20C (68F): No Data pH: 4.0 6.0APPEARANCE, ODOR and COLOR: This product is a tan to brown viscous liquid with a slight odor.STABILITY: Stable.DECOMPOSITION PRODUCTS: Thermal degradation produces carbon dioxide, carbon monoxide and oxides of phosphorous. MATERIALS WITH WHICH SUBSTANCE IS INCOMPATIBLE: Strong oxidizing agents and strong reducing agents POSSIBILITY OF HAZARDOUS REACTIONS: Will not occur.CONDITIONS TO AVOID: Incompatible materials.

22Nature's Cure- Purely Organic SAPhysical and Chemical PropertiesReactivity and Stability TOXICITY DATA: No Data Available for the components of this productsSUSPECTED CANCER AGENT: The following components of this product are listed by agencies tracking the carcinogenic potential of chemical compounds as follows: None knownIRITANCY OF PRODUCT: Contact of this product can cause slight skin and eye irritation.SENSITIZATION TO THE PRODUCT: These products are not known to cause human skin or respiratory sensitization.REPRODUCTIVE TOXICITY INFORMATION: Listed below is information concerning the effects of this product and its components on the human reproductive system. Mutagenicity: The components of this product are not reported to produce mutagenic effects in humans. Embryotoxicity: The components of this product are not reported to produce embryotoxic effects in humans. Teratogenicity: The components of this product are not reported to produce teratogenicity effects in humans. Reproductive Toxicity: The components of this product are not reported to produce reproductive effects in humans.

ALL WORK PRACTICES MUST BE AIMED AT ELIMINATING ENVIRONMENTAL CONTAMINATION.

ENVIRONMENTAL FATE: This material is not expected to be hazardous to the environment.ENVIRONMENTAL TOXICITY: This material is not expected to be toxic to aquatic life.PREPARING WASTES FOR DISPOSAL: Waste disposal must be in accordance with appropriate U.S. Federal, State, and local regulations, those of Canada, Australia, EU Member States and Japan.

23Nature's Cure- Purely Organic SAToxicological Information Ecological Information Disposal Considerations 23INHALATION FIRST AID: Not expected as a result of this product, however if breathing problems occur remove victim to fresh air. If persistent breathing difficulty occurs, get medical attention.SKIN CONTACT FIRST AID: Wash skin with plenty of soap and water after handling or contact. Get medical attention if irritation develops or persists.EYE CONTACT FIRST AID: If contact with eyes, flush eyes with plenty of water for at least 15 minutes lifting upper and lower eyelids occasionally. Get medical attention if irritation persists.INGESTION: Routine use of this product is not expected to cause any situation which could lead to ingestion. If this product is swallowed, CALL PHYSICIAN OR POISON CONTROL CENTER FOR MOST CURRENT INFORMATION.If professional advice is not available, do not induce vomiting. Never induce vomiting or give diluents (milk or water) to someone who is unconscious, having convulsions, or unable to swallow.MEDICAL CONDITIONS AGGRAVATED BY EXPOSURE: None known

RECOMMENDATIONS TO PHYSICIANS: Treat symptoms and eliminate overexposure.24Nature's Cure- Purely Organic SAFirst-Aid Measures 25Nature's Cure- Purely Organic SACONCLUSIONSNature's Cure- Purely Organic SA26After observing all the outstanding results that Natures Cure can provide to crops we are proud to introduce an organic product whose concentrated foliar application material will enhance plant defenses and improves soil moisture retention.

Natures Cure will give the growers the opportunity to solve many problems that they face every season within their production:

Delay decay and ripening of the climacteric fruits.Prevent and treat Frost damage in plant. Delay color changes and increase shelf-life in fruits and vegetables.Stimulates nutrients uptake and enhance plant growth.Reduce weight lost and maintain plant texture.

Extension of storage time accomplished by using Natures Cure will facilitate the exports of fruits to long distance markets by sea and thereby the cost of exportation will be reduced.

Will make the production of fruits and vegetables more competitive in the worlds market.

ConclusionsNature's Cure- Purely Organic SA27 Products do not have to stay in local markets. By using Natures Cure the growers will be able to export new products to new markets.

Chemical treatments and advance equipments are costly and they do not guarantee that the production will be save or recovered.

Since Natures Cure improves the nutrients absorbance to the roots the crops grow up faster and healthier than by using any other similar treatment.

Grower no longer worry about frost damage and the loss of overall production due to climate changes.

Since it is an organic product no health hazard or special restriction has to be taken.

Overall, Natures Cure is a very simple and reliable technique that will bring great benefits to the agricultural field.

ConclusionsNature's Cure- Purely Organic SA28Contacts

Ral Muoz Authorized Distributor Phone : 9933-7597 (Chile) (201) 942-0400 (EU)Address : Avenida Pocuro 2521 Providencia Santiago, ChileE-mail: [email protected]

Gladys Michelle Reyes Biochemist Phone: (201) 963-1028 Ext:104 (EU)E-mail :[email protected]