CHALLENGES AND SOLUTIONS ADOPTED FOR THE CREATION OF A COMPLEX AND MULTICENTRE PROSPECTIVE COLLECTION OF TISSUE SAMPLES TO EVALUATE THE USABILITY OF BIOMARKERS IN BIOSPECIMEN RESEARCH Background 2nd Biospecimen Research Symposium: Focus on Quality and Standards Berlin, Germany | February 5-6, 2019 Esteva-Socias M 1,2 , Artiga MJ 2,3 , Astudillo A 2,4 Bahamonde O 2,5 , Bermudo R 2,6 , Belar O 2,7 , Campos Y 8 , Castro E 2,7 , Escalante M 2,9 , Escámez T 2,10 Guerrero C 11 , Gómez E 11 , Iglesias M 2,12 ,Jauregui-Mosquera L 2,13 , Novoa I 2,14 , Peiró-Chova L 2,5 , Rábano A 2,15 , Rebolledo A 11 ,Rejón JD 2,16 , Ruíz-Miró M 2,17 , Villar V 2,13 , Zazo S 2,18 , Villena C 1,2 . 1 Pulmonary Biobank Consortium, CIBER of Respiratory Diseases (CIBERES) - ISCIII (Madrid), Institut d’Investigació Sanitària de les Illes Balears (IdISBA) - Hospital Universitari Son Espases, Mallorca. 2 Spanish Biobank Network. 3 CNIO Biobank, Spanish National Cancer Research Center (CNIO), Madrid. 4 BioBanco del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo. 5 INCLIVA Biobank, Valencia. 6 HCB-IDIBAPS Biobank, Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), Barcelona. 7 Basque Biobank; The Basque Foundation for Health Innovation and Research (BIOEF), Bilbao. 8 Biobanco Hospital Virgen de la Salud (BioB-HVS). 9 Biobanco A Coruña. 10 Biobanco en Red de la Región de Murcia. 11 Biobanco Hospital Universitario Fundación Alcorcón. 12 MARBiobanc, Biobanco del Consorci Mar Parc de Salut de Barcelona. 13 University of Navarra’s Biobank IdiSNA, Pamplona. 14 Biobanco Hospital Universitario Vall d’Hebron, Vall d´Hebron Institut de Recerca (VHIR), Barcelona. 15 Departamento Neuropatología, Banco de Tejidos Cien, Fundación Centro Investigación Enfermedades Neurológicas (CIEN), Madrid. 16 Andalusian Public Health System Biobank, Granada. 17 IRBLleida Biobank, Instituto de Investigaciones Biomédica de Lleida-Fundación Dr. Pifarre, Lleida. 18 Fundación Jiménez Díaz Biobank, Madrid. Seventeen biobanks of Spanish Biobank Network (SBN) as a part of the ISBER Biospecimen Science Working Group are developing a collaborative prospective collection of tissue samples with controlled pre-analytical variables. The main objective of this collaboration is to measure the impact of pre-analytical variables, mainly cold ischemia and fixation time, on tissue samples. Methods Sixteen tissue blocks of 0,5 cm 3 per organ were collected per patient [8 snap frozen and 8 formalin-fixed paraffin-embedded (FFPE)] with controlled cold ischemia time (CIT). During CIT, samples are kept at room temperature in a sealed box with a damp gauze to prevent them drying out. Past the scheduled time, the fresh samples are either fast freezed and stored at -80ºC or formalin fixed, paraffin-embedded and stored in a dry and dark place at 2-10ºC (Figure 1). Figure 1. Workflow of collection and downstream processing. Conclusions A network with high heterogeneity of biobanks allows obtaining a wide diversity of organs for Biospecimen Research. However, a common procedure for collaborative multicenter sample collection has been needed, to obtain a well-characterized tissue samples available with controlled pre-analytical variables for wide Biospecimen Research, and to face all the technical challenges. The main ones have been identified and described and addressed, being mainly solved through a feasibility calendar of sample collection, as well as the participation of at least 2 well-trained people. Progress and management Figure 2. Summary of the main stages in the development of the collaborative project. Figure 3. Biobanks of the SBN participating in the prospective collection and tissues collected. Sample collection was initiated in Spain on 2018 (Figure 2) through a collaborative project between the SBN and the Integrated Biobank of Luxembourg, establishing the collection conditions and requirements. Currently, the collection process is being executed in the 16 participating biobanks, and tissue samples are collected from 10 different organs as is shown in Figure 3. Results Figure 4. Scheme of the whole protocol for sample collection, including cold ischemia time-points, personnel shifts and histological processing runs. Figure 5. Feasibility calendar of sample collection during working days and weekend to be able to perform the whole established protocol.
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CHALLENGES AND SOLUTIONS ADOPTED FOR THE CREATION OF A COMPLEX AND MULTICENTRE
PROSPECTIVE COLLECTION OF TISSUE SAMPLES TO EVALUATE THE USABILITY OF BIOMARKERS IN
BIOSPECIMEN RESEARCH
Background
2nd Biospecimen Research Symposium:
Focus on Quality and Standards
Berlin, Germany | February 5-6, 2019
Esteva-Socias M1,2, Artiga MJ2,3, Astudillo A2,4 Bahamonde O2,5, Bermudo R2,6, Belar O2,7, Campos Y8, Castro E2,7, Escalante M2,9, Escámez T2,10 Guerrero C11, Gómez E11,
Iglesias M2,12,Jauregui-Mosquera L2,13, Novoa I2,14, Peiró-Chova L2,5, Rábano A2,15, Rebolledo A11,Rejón JD2,16, Ruíz-Miró M2,17, Villar V2,13, Zazo S2,18, Villena C1,2.
1 Pulmonary Biobank Consortium, CIBER of Respiratory Diseases (CIBERES) - ISCIII (Madrid), Institut d’Investigació Sanitària de les Illes Balears (IdISBA) - Hospital Universitari Son Espases, Mallorca. 2 Spanish Biobank Network. 3 CNIO Biobank, Spanish National Cancer Research
Center (CNIO), Madrid. 4 BioBanco del Principado de Asturias, Hospital Universitario Central de Asturias, Oviedo. 5 INCLIVA Biobank, Valencia. 6 HCB-IDIBAPS Biobank, Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), Barcelona. 7Basque Biobank; The Basque
Foundation for Health Innovation and Research (BIOEF), Bilbao. 8 Biobanco Hospital Virgen de la Salud (BioB-HVS). 9 Biobanco A Coruña. 10 Biobanco en Red de la Región de Murcia. 11 Biobanco Hospital Universitario Fundación Alcorcón. 12 MARBiobanc, Biobanco del Consorci Mar
Parc de Salut de Barcelona. 13University of Navarra’s Biobank IdiSNA, Pamplona. 14 Biobanco Hospital Universitario Vall d’Hebron, Vall d´Hebron Institut de Recerca (VHIR), Barcelona. 15Departamento Neuropatología, Banco de Tejidos Cien, Fundación Centro Investigación
Enfermedades Neurológicas (CIEN), Madrid. 16 Andalusian Public Health System Biobank, Granada. 17 IRBLleida Biobank, Instituto de Investigaciones Biomédica de Lleida-Fundación Dr. Pifarre, Lleida. 18 Fundación Jiménez Díaz Biobank, Madrid.
Seventeen biobanks of Spanish Biobank Network (SBN) as a part of the ISBER
Biospecimen Science Working Group are developing a collaborative prospective
collection of tissue samples with controlled pre-analytical variables. The main objective
of this collaboration is to measure the impact of pre-analytical variables, mainly cold
ischemia and fixation time, on tissue samples.
Methods Sixteen tissue blocks of 0,5 cm3 per organ were collected per patient [8 snap frozen
and 8 formalin-fixed paraffin-embedded (FFPE)] with controlled cold ischemia time
(CIT). During CIT, samples are kept at room temperature in a sealed box with a damp
gauze to prevent them drying out. Past the scheduled time, the fresh samples are
either fast freezed and stored at -80ºC or formalin fixed, paraffin-embedded and
stored in a dry and dark place at 2-10ºC (Figure 1).
Figure 1. Workflow of collection and downstream processing.
Conclusions
A network with high heterogeneity of biobanks allows obtaining a wide diversity of
organs for Biospecimen Research. However, a common procedure for collaborative
multicenter sample collection has been needed, to obtain a well-characterized tissue
samples available with controlled pre-analytical variables for wide Biospecimen
Research, and to face all the technical challenges. The main ones have been identified
and described and addressed, being mainly solved through a feasibility calendar of
sample collection, as well as the participation of at least 2 well-trained people.
Progress and management
Figure 2. Summary of the main stages in the development of the collaborative project. Figure 3. Biobanks of the SBN participating in the prospective collection and tissues collected.
Sample collection was initiated in Spain on 2018 (Figure 2) through a collaborative project
between the SBN and the Integrated Biobank of Luxembourg, establishing the collection
conditions and requirements. Currently, the collection process is being executed in the 16
participating biobanks, and tissue samples are collected from 10 different organs as is shown in
Figure 3.
Results
Figure 4. Scheme of the whole protocol for
sample collection, including cold ischemia
time-points, personnel shifts and histological
processing runs.
Figure 5. Feasibility calendar of sample
collection during working days and weekend to
be able to perform the whole established
protocol.
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