Production of Erythropoietin in Pichia Pastoris Eporis – Team 15 ProblemBackgroundTeamDesign NormsDesign Alternatives.

Production of Erythropoietin in Pichia

PastorisEporis – Team 15

ProblemBackgroundTeam Design NormsDesign Alternatives

Team Eporis

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Abby Leistra Stephen Tubergen Zion Lee Nick Giles

ProblemBackgroundTeam Design NormsDesign Alternatives

Background: What is Erythropoietin?

•Erythropoietin (EPO) is a glycoprotein hormone•Promotes red blood cell production•Naturally made in kidneys•Treats anemia caused by• Chronic kidney disease

• Cancer chemotherapy

• HIV therapy

• $13 Billion Market

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glycam.org

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Problem

•An eight-week treatment can cost up to $11000

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• Expression: Chinese Hamster Ovary (CHO) Cells

http://nikon2.magnet.fsu.edu

• Well studied

• Expensive feed

• Slow growth rate

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Solution?

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• Expression: Pichia Pastoris cells

• Relatively new

• Cheap feed (methanol)

• Fast growth rate

2013.igem.org

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Design Alternatives

•Pichia Pastoris Strain

• Turn on and off right genes

•Erythropoietin Structure

• Glycosylation and other modifications

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glycam.org

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Design Alternatives

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• Bioreactors

• Separation Units

Mianbin Wu, et. al. (2011). DOI: 10.5772/20246.

ProblemBackgroundTeam Design NormsDesign Alternatives

Design Norms

•Stewardship

•Transparency

•Trust

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Questions?