Proceedings of Nanotechnology International Forum Rusnanotech 2010, November 1-3, Moscow Biodistribution and stability of quantum dots in mouse digestive tract following per os administration. Salykina Y.F. 1 , Zherdeva V.V. 1 , Dezhurov S.V. 2 , Wakstein M.S. 2 , Savitsky A.P. 1 1 A.N. Bakh Biochemistry Institute of RAS, 2 Applied Acoustics Research Institute, Center of High Technologies [email protected], [email protected] Quantum dots (QD) are 1–10-nm semiconductor nanocrystals with unique optical и photophysical properties such as size- and composition-tunable emission, high brightness, narrow emission bands, and high resistance to photobleaching. Nowadays, when the range of the industry of the products containing nanoparticles expands, there is a possibility for QDs to get into organism via a variety of routes (by breathing, intravenous, per os or through skin). Oral exposure can be anticipated to occur through direct (e.g., drug delivery), incidental (e.g., water, food) or indirect (e.g., clearance from the lungs) routes. The stability of nanoparticles in the gastrointestinal tract (GI) is expected to affect potential toxicity of QDs’ core materials. GOAL Studying of the distribution of CdSe-core, ZnS-capped QDs, modified by various functional ligands, moving through the digestive tract of mice the effect of ligand modification on the stability and photoluminescence properties of QDs under the influence of surrounding media of the GI. OBJECTS RESULTS QDs coated by 3-mercaptopropionic acid emitting at 630 nm (QDs MPA) QDs PolyT-APS emitting at 678 nm have been obtained by our original method from QDs PolyT by additional coating with 3- aminopropyltriethoxysilane METHODS The colloidal solution of different types QDs in 20mM sodium-phosphate buffer (рН=8) were administrated to nude and CD-1mice per os. The optical fluorescent in vivo imaging was obtained on iBox (UVP, USA) using correspondent band-pass excitation (502-547 nm) and emission filters (570-640 nm or 670-720 nm). Fluorescent spectra of tissue samples, feces and urine were obtained postmortem by the method of fluorescent laser spectrometry using multispectral fluorescent system Spectr-Cluster (Institute of General Physics of RAS) with laser excitation source. 1 h Contr 10 20 30 min 2 h Stomach Time after administration Duodenum Intestine 0 500 1000 1500 2000 2500 3000 3500 580,00 630,00 680,00 Fluorescence intensity, a.u. Wavelength, nm QD MPA in buffer stomach 2 h after administration of QD MPA stomach control excrements 24 h after administration of QD MPA excrements control 0 0,2 0,4 0,6 0,8 1 1,2 538,00 638,00 738,00 F/Fмакс Wavelength, nm Normalized spectra stomach 2 h after administration of QD MPA excrements 24 h after administration of QD MPA QD MPA in buffer 0 200 400 600 800 1000 1200 1400 1600 640 690 740 Fluorescence intensity, a.u. Wavelength, nm QD PolyT-APS in buffer stomach 2 h (without autofluorescence) stomach control blind intestine 6 h 0 500 1000 1500 2000 2500 3000 3500 650 700 750 Fluorescence intensity, a.u. Wavelength, nm excrements 2 h excrements 4 h excrements 6 h excrements 24 h excrements control QD PolyT-APS in buffer control 10 min 30 min 1 h 2 h 3 h 4 h 5 h This study was supported by grants № 01.648.11.3003 and № 01.648.11.3006 from the Federal Agency for Science and Innovation. This work was partially supported by grant № 09-04-12263 from the RFBR. These preliminary results have shown possibility of use of QDs PolyT-APS for studying in vivo processes in digestive system because of their high stability under conditions of the gastrointestinal tract. QDs МРА presumably lose their nanostructure, being submitted by influence of digestive system; tend to aggregation and irreversible degradation of a ZnS-shell. CONCLUSIONS QD MPA QD PolyT-APS
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Proceedings of Nanotechnology International Forum Rusnanotech 2010, November 1-3, Moscow
Biodistribution and stability of quantum dots in mouse digestive tract following per os administration.
Salykina Y.F.1, Zherdeva V.V.1, Dezhurov S.V. 2, Wakstein M.S. 2, Savitsky A.P.1
1 A.N. Bakh Biochemistry Institute of RAS, 2 Applied Acoustics Research Institute, Center of High [email protected], [email protected]
Quantum dots (QD) are 1–10-nm semiconductor nanocrystals with unique optical и photophysical properties such as size- andcomposition-tunable emission, high brightness, narrow emission bands, and high resistance to photobleaching.Nowadays, when the range of the industry of the products containing nanoparticles expands, there is a possibility for QDs to getinto organism via a variety of routes (by breathing, intravenous, per os or through skin). Oral exposure can be anticipated to occurthrough direct (e.g., drug delivery), incidental (e.g., water, food) or indirect (e.g., clearance from the lungs) routes. The stability ofnanoparticles in the gastrointestinal tract (GI) is expected to affect potential toxicity of QDs’ core materials.
GOAL Studying of
the distribution of CdSe-core, ZnS-capped QDs, modified by various functional ligands, moving through the digestive tract of mice the effect of ligand modification on the stability and photoluminescence properties of QDs under the influence of surroundingmedia of the GI.
OBJECTS
RESULTS
QDs coated by 3-mercaptopropionic acid emitting at 630 nm (QDs MPA)
QDs PolyT-APS emitting at 678 nm have been obtained by ouroriginal method from QDs PolyT by additional coating with 3-aminopropyltriethoxysilane
METHODS The colloidal solution of different types QDs in 20mM sodium-phosphate buffer (рН=8) were administrated to nude and CD-1mice per os. The optical fluorescent in vivo imaging was obtained on iBox (UVP, USA) using correspondent band-pass excitation (502-547 nm) andemission filters (570-640 nm or 670-720 nm). Fluorescent spectra of tissue samples, feces and urine were obtained postmortem by the method of fluorescent laser spectrometry usingmultispectral fluorescent system Spectr-Cluster (Institute of General Physics of RAS) with laser excitation source.
1 h
Control
10 min
20 min
30 min
2 h
Stomach
Time after
administration
Duodenum
Intestine
0
500
1000
1500
2000
2500
3000
3500
580,00 630,00 680,00
Flu
ore
sce
nce
inte
nsi
ty, a
.u.
Wavelength, nm
QD MPA in buffer
stomach 2 h after administration of QD MPA
stomach control
excrements 24 h after administration of QD MPA
excrements control
0
0,2
0,4
0,6
0,8
1
1,2
538,00 638,00 738,00
F/Fм
акс
Wavelength, nm
Normalized spectra
stomach 2 h after administration of QD MPA
excrements 24 h after administration of QD MPAQD MPA in buffer
0
200
400
600
800
1000
1200
1400
1600
640 690 740
Flu
ore
sce
nce
inte
nsi
ty, a
.u.
Wavelength, nm
QD PolyT-APS in buffer
stomach 2 h (without autofluorescence)
stomach control
blind intestine 6 h
0
500
1000
1500
2000
2500
3000
3500
650 700 750
Flu
ore
sce
nce
inte
nsi
ty, a
.u.
Wavelength, nm
excrements 2 h
excrements 4 h
excrements 6 h
excrements 24 h
excrements control
QD PolyT-APS in buffer
control 10 min 30 min 1 h
2 h 3 h 4 h 5 h
This study was supported by grants № 01.648.11.3003 and № 01.648.11.3006 from the Federal Agency for Science and Innovation. This work was partially supported by grant № 09-04-12263 from the RFBR.
These preliminary results have shown possibility of use of QDs PolyT-APS for studying in vivo processes indigestive system because of their high stability under conditions of the gastrointestinal tract. QDs МРАpresumably lose their nanostructure, being submitted by influence of digestive system; tend to aggregationand irreversible degradation of a ZnS-shell.
CONCLUSIONS
QD MPA
QD PolyT-APS
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