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Invisible mineral shield for a long-lasting protection for

skin care & oral care applications

Cosmetic BIOprotection

Oris

Biomimetic Hydroxyapatite functionalizedwith cationic surfactant (QAC)

Technology based on the use of the HAP, a remineralizing active, also acting as delivery system able to deliver a hygienizing surfactant in the oral mouth througha time controlled kinetics (4 hours).

• International patent• Hygienizing active for toothpaste and mouthwashes: remineralizing HAP

active vs biological agents• Long lasting bio-protection against infectious agents• Single application protects oral mouth up to 4 hours• Proven activity vs Bacteria and Viruses (including S. Mutans and Coronavirus)• Local microbiota respectful

NEED OF ORAL CARE PRODUCTS ABLE

TO ENSURE HYGIENE

CONTAINING INGREDIENTS TO:

• EXTEND THE PROTECTIVE ACTIVITY

• FORM ALCOHOL-FREE PRODUCT

• REDUCE SENSITIVITY AND STRENGTHEN TEETH

PRODUCT INNOVATION TRENDS

EMERGENCY TIME

SOURCE: ORAL CARE MARKET FORECAST TO 2028 - COVID-

19 IMPACT AND GLOBAL ANALYSIS BY PRODUCT; DISTRIBUTION CHANNEL, AND GEOGRAPHY

MEET THE NEED: OUR INNOVATIVE PROPOSAL

INNOVATIVE COSMETIC INGREDIENT FOR THE

“NEW NORMAL” TO BOOST YOUR POST PANDEMIC

ORAL CARE AND PERSONAL HYGIENE PROTECTION

Oris

DESIGNING KALHYGIENIC

FUNCTIONALIZED BIOMIMETIC

HYDROXYAPATITE

+

CATIONIC SURFACTANTQUATERNARY AMMONIUM COMPOUND

STOIOCHIOMETRIC VS BIOMIMETIC HYDROXYAPATITE

BIOMIMETICHYDROXYAPATITE

• LOWER CRYSTALLINITY DEGREE

• ADAPTED MORPHOLOGY, OPTIMIZED

FOR TOOTH ADHESION

• HIGHER REACTIVITY

• HIGHER BIOACTIVITY

• OPTIMIZED RELEASE OF

INCORPORATED ACTIVES

• LONG LASTING TOOTH REMINERALIZATION

STOICHIOMETRIC HYDROXYAPATITE

• HIGHER CRYSTALLINITY DEGREE

• LOW ADHESION TO TOOTH SURFACE

• LOWER REACTIVITY

• LOWER BIOACTIVITY

• VERY SLOW RELEASE OF

INCORPORATED ACTIVES

• POOR REMINERALIZING ACTION

CATIONIC SURFACTANT- QUATERNARY AMMONIUM COMPOUND (QAC)

WORKS AS CATIONIC SURFACTANT

BY IMPACTING BIOLOGICAL WALLS AND MEMBRANES OF

MICROORGANISMS AND KEEPING THE SKIN CLEAN

PROMOTES INACTIVATION OF VIRUSES AND BACTERIA BY

DESTROYING THE VIRAL ENVELOPE OR BACTERIAL MEMBRANE(THIS MECHANISM OF ACTION DOES NOT INDUCE RESISTANCE SELECTION)

WIDELY USED IN COSMETICS AND FOOD AS PRESERVATIVE AND

CONTAINED IN ORAL CARE PRODUCTS, IT POSSESSES AN

OPTIMAL SAFETY PROFILE

PROTECTIVE

PREVENTIVE

SAFE

SUGARS ARE METABOLIZED BY BACTERIA THUS PRODUCING ACIDS

THAT DECREASE PH - STEPHAN’S CURVE DESCRIBES THE VARIATION

OF PH IN THE MOUTH AFTER A MEAL

• WHEN USED IN ORAL CARE FORMULATIONS

(MOUTHWASHES, TOOTHPASTES, DENTAL GELS, ETC.) KALHYGIENIC SETTLES ON TOOTH ENAMEL AND

SLOWLY RELEASES QAC

• DECREASE OF PH IN MOUTH AFTER A MEAL

PROMOTES THE RELEASE OF QAC FROM HAP

↑QAC RELEASE

BIOMIMETIC HAP AS QAC CONTROLLED DELIVERY SYSTEM

STEPHAN’S CURVE SINGLE SUGAR EXPOSURE

BIOMIMETIC HYDROXYAPATITE DEPOSITED ON TOOTH

REMINERALIZING & ANTI-HYPERSENSITIVITY ACTION

KALIDENT BIOMIMETIC HAP acts as a filler, by restoring minute cracks and scratches on tooth surface; its deposition allows to form a physiological reservoir that boosts in long term and ‘’on demand’’ the remineralization of scratches and lesions(@5%)

KALICHEM’S TESTS: EFFICACY OF KALHYGIENIC

REF. TEST TYPE PROPERTIES TIMETARGET MICRO

ORGANISM%

OF USE

TRANSMISSION

ELECTRON

MICROSCOPY

ACTIVITY ON

BACTERIAL

MORPHOLOGY

AND VIRAL

ENVELOPE

1 HRSTREPTOCOCCUS MUTANS

10%

CORONAVIRUS

4 HRSSTREPTOCOCCUS MUTANS

CORONAVIRUS

INFECTIVITY

ASSAY (PREN14476:2011)

VIRUCIDAL

ACTIVITY

1 HR

CORONAVIRUS

4 HRS

ACTIVITY OF KALHYGIENIC VS S. MUTANS

CONTROL KALHYGIENIC1 H

4 H

S. Mutans undergoes a deep alteration in its morphology; membrane becomes rough and shape irregular

TRANSMISSION ELECTRON MICROSCOPY (EM) STUDIES SHOWED THAT

• KALHYHIENIC (10%) WORKS EFFICIENTLY IN DEEPLY ALTERING THE MORPHOLOGYOF STREPTOCOCCUS MUTANTS (RESPONSIBLE FOR DENTAL CAVITY)

• AFTER 4 HOURS THE ALTERATION RESULTS STILL VERY EVIDENT

CONTROL KALHYGIENIC1 H

4 H

TRANSMISSION ELECTRON MICROSCOPY (EM) STUDIES SHOWED THAT

• KALHYGIENIC (10%) WORKS EFFECTIVELY IN DISRUPTING VIRAL ENVELOPE

OF A SELECTED STRAIN OF CORONAVIRUS AFTER 1 HOUR TREATMENT

• AFTER 4 HOURS ALTERATION RESULTS SIGNIFICANTLY INCREASED

Intact virus undergoes a deep alteration in morphology leaving debris and expandend round structures

ACTIVITY OF KALHYGIENIC VS CORONAVIRUS

EVALUATION OF VIRUCIDAL ACTIVITY ACCORDING TO EUROPEAN NORM PREN 14476:2011THE ASSAY ALLOWS TO EVALUATE THE ABILITY OF THE TEST SUBSTANCE TO INHIBIT GROWTH AND REPLICATION OF VIRAL PARTICLES

IN TARGET CELLS

TEST: ABILITY TO REDUCE REPLICATION OF A SELECTED STRAIN OF

CORONAVIRUS IN CELLS AFTER

1 HOUR AND 4 HOURS TREATMENT RESPECTIVELY

VIRAL PARTICLES WERE TITRED BY USING KARBER METHOD

VIR

UC

IDA

LA

CTI

VIT

Y[%

]

1 HR 4 HRS

99% 99%

INFECTIVITY ASSAY VS CORONAVIRUS

KALHYGIENIC (10%)

• does not alter the composition of the normal oral flora • QAC does not allow potentially pathogenic bacteria in the mouth*

• QAC Reduces the production of volatile sulfur compounds(VSCs) by oral bacteria thus could reduce halitosis**

* Radford et Al. 1997** Liu et Al. 2013

BENEFITS OF KALHYGIENIC IN ORAL CARE

• ensures a long-lasting effect by gradually releasing active concentrations of QAC in the mouth following a pH dependent kinetics

KALHYGIENIC

• DEEP AND LONG-LASTING

PROTECTION

• BIO-PROTECTION BARRIER

AGAINST HARMFUL GERMS

• REDUCTION OF TOOTH SENSITIVITY

• ENAMEL REMINERALIZATION

• PREVENTION OF BAD BREATH(REDUCTION OF MICROORGANISMS CAUSING

BAD ODOURS)

ORAL CARE CLAIMS

• CARIES PREVENTION

• MICROBIOTA RESPECTFUL

• ANTI-BACTERIAL

• REDUCTION OF PLAQUE AND TARTAR

BUILD-UP

• PREVENTION OF GUM INFLAMMATION

TOOTHPASTE

PHASE INGREDIENTS %

A

GLYCERIN 32.50

CELLULOSE GUM 1.65

STEVIA REBAUDIANA EXTRACT, ERYTHRITOL 0.25

B AQUA 37.50

C

SILICA 10.00

HYDRATED SILICA 11.50

SODIUM FLUORIDE 0.10

D KALHYGIENIC 5.00

ESODIUM OLIVOYL GLUTAMATE, DISODIUM COCOYL

GLUTAMATE, AQUA1.50

F AROMA, COLORANT, PRESERVATIVE AS NEEDED

MIX THE INGREDIENTS OF

PHASE A UNTIL A HOMOGENEOUS

PASTE IS OBTAINED. ADD PHASE A TO

PHASE B, STIRRING AND HOMOGENIZING UNTIL A

SLIGHTLY VISCOUS GEL WITH NO LUMPS IS

FORMED. ADD THE POWDERS OF PHASE C ONE BY

ONE, STIRRING AND HOMOGENIZING AFTER EVERY

ADDITION: THE PRODUCT SHOULD PRESENT ITSELF

AS A PERFECTLY HOMOGENEOUS VISCOUS PASTE

ADD D AND HOMOGENIZE.ADD THE INGREDIENTS OF PHASE E AND PHASE F, STIRRING AND HOMOGENIZING AFTER EVERY

ADDITION.THE VISCOSITY OF THE TOOTHPASTE

CAN BE EASILY ENHANCED OR

REDUCED BY CHANGING THE

KIND OF CELLULOSE GUM

USED.

LONG LASTING • CLEAN SENSATION • DEEP HYGIENE • BIOPROTECTION