Results Materials and methods Acknowledgments ZARATE-MORALES, A., GASPAR-CARCAMO, R.E., LOPEZ-RODRIGUEZ, V., FLORES-MORENO, A., TREJO-BALLADO, F., AVILA-RODRIGUEZ, M.A. Unidad PET, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 , D.F. México. Introduction Conclusions This work was supported by CONACYT Grant 121652, UNAM-DGAPA-PAPIIT TA200512 and International Atomic Energy Agency RC16467. RDS 111 in 2008 (8 targets, 40 A) Acceptance test of the system was performed by irradiating the gold disk for 2h at 40μA without apparent signal of damage. The solid target system have been tested successfully producing Ga and Cu radioisotopes by irradiating electrodeposited targets of Zn and Ni, respectively. Nevertheless the success, some problems: Poor cooling-water system Difficulty for irradiation of foils Although not common, target jamming after bombardment Eclipse HP in 2011 (4 targets, 60A) A dual beam line (BL) RDS 111 cyclotron for radionuclide production was installed at the National Autonomous University of Mexico in 2001. One of the BL´s was upgraded to Eclipse HP in 2008 and the second BL was recently upgraded (2011) with the option for the irradiation of solid targets for the production of metallic radioisotopes. Automatic and manual operation of the solid target system is possible from the cyclotron control software and the graphical user interface (GUI) shows the status of all involved parameters. nat Zn or nat Ni targets were prepared by electrodeposition of ZnCl 2 or Ni(NO 3 ) 2 on gold disk. Zn Target (x10) 5.5 V, 30 min (70 – 50 mA) Ni Target (x10) 3.4 V, 9 h (30 – 20 mA) In vivo serial microPET images demonstrated rapid C6 tumor uptake of 66 Ga-DOTA-E-[c(RGDfK) 2 ] A solid target system was fitted onto an existing self-shielded 11 MeV cyclotron. In our experience the system is capable to produce useful quantities of metallic PET radionuclides for preclinical applications, mainly limited by the manual removal of the target that increases radiation exposure to personnel. 11 MeV Protons Cooling water (7 °C) nat Zn or nat Ni targets were left on the tungsten container over 17 and 1 h respectively, so that short- lived products could decay. nat Zn targets were bombarded (10 to 40 μA) 1 h nat Ni targets were bombarded (5 μA) 30 min Micro-derenzo phantom and mice with subcutaneously growing C6 xenografts microPET images were acquired using a microPET scanner (Focus 120 Concorde Microsystem). (Focus 120) 66 Ga (56% + , E max = 4.2 MeV) The radiochemical separation was achieved using cation-exchange chromatography At the beginning the most common problem with the Zn- targets was due to poor quality electrodeposition but now it is under control. 1 h biodistribution 3 h biodistribution 20 h biodistribution 24h biodistribution