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bioMrieux
ImmunoassaySystems
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V ITEKI MMUNO
DIAGNOSTIC
A SSAYS YSTEM
- 1-1
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VIDAS - The Concept
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SPR coated with antibodies
or antigens, serving also as
pipeting device
strip containing all the
ready-to-use reagents
UNIQUE and original
unitaryconcept, avoidingany cross contamination.
1 SPR + 1 strip = 1 patient
VIDAS - Disposables
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VIDAS : an easy loading
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VIDAS : a dynamic process
Strip
SPR
Time & delays Movements Volumes
}depend on the PROTOCOL
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Photodiode
Washingbuffers Substrate
Sample
Diluent
(Pre-wash) Conjugate
Example of a reaction
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ECHANTILLON TAMPON CONJUGUE SUBSTRAT
DE LAVAGE
E
E
E
E
E
E
E
Antigen detection : example of procedure
Cycledin and out
SPR
Target
organism
sample washing conjugate substratebuffer
IMMUNOLOGICAL REACTION ENZYMATIC REACTION
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Principle for an antigen detection test
sandwich principle
Bound Ab
Ag to be detected
Alkaline phosphatase-
labeled Ab
4-methyl umbelliferyl phosphate
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Filters
Lenses
PhotodiodeXenonFlashLamp
VIDAS : reading in fluorescence
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Alkaline phosphatase-
labeled hapten
Hapten to be detected
Bound Ab
COMPETITION
Detection of haptens
4-methyl umbelliferyl phosphate
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4-methyl umbelliferyl phosphate
Alkaline phosphatase-
labeled Ab
Ab to be
detected
Bound Ag
ELISA INDIRECT
Serologies
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Ab to be
detected
Ag in liquid medium
Anti-IgM
IMMUNOCAPTURE
Detection of specific IgM
4-methyl umbelliferyl phosphate
alkaline phosphatase-
labeled Ab
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A memorized calibration
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Calibration (of quantitative tests)
1 - The Master Curve (reference curve)
is performed by QC Lab
0
1000
2000
3000
4000
5000
6000
0 6 12 18 24 30 36 42 48 54
R
FV
Concentration
+++ ++++
++++++++
++++++
++++
++++
+++++
+++++
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1 - Results are summarized on a card
07714_A30406
VIDAS
LH
1 AQ8TE5K2 J21QY3Y
3 6VO6HJ6
4 N65CHD
5 JFK355
6 4
7 4G54
8
9
10
11
12
13
14 15
16 C
Assay:LH
Striplot:030929-0
StandardR
FVValues:
149,635,2215,5061
Calibration
method:
7
Calibration
(S1)DoseValue:
48.2mUI/ml
ControlC1
DoseValueRange:
5.27
--
7.37mUI/ml
Calibrator
(S1)RFVrange:
1858--3318
Max.CV%:
10.0
bioMrieu
xsa
69280
Marc
yl'EtoileFrance
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MLE card description
2 The MLE is to be read by VIDAS
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2
The MLE is to be read by VIDAS,to store the master curve
0 7 714
_A
30406 VIDAS LH
1
AQ8TE5K
2
J21QY3Y
3
6VO6HJ6
4
N65CHD
5
JFK355
6
4
7
4G54
8
9
10
11
12
13
14
15
16
C
Assay : LH Strip lot : 030929-0
S ta nd ard RF V Va lu es : 1 49 , 63 5, 22 15 ,5 06 1
Calibration method : 7
Calibration(S1) Dose Value : 48.2 mUI/ml
Control C1 Dose Value Range : 5.27 -- 7.37mUI/ml
Calibrator (S1) RFV range : 1858--3318 Max. CV%:10.0
bioMrieux sa 69280 Marcy l'Etoile France
0 7 714
_A
30406 VIDAS LH
1
AQ8TE5K
2
J21QY3Y
3
6VO6HJ6
4
N65CHD
5
JFK355
6
4
7
4G54
8
9
10
11
12
13
14
15
16
C
Assay : LH Strip lot : 030929-0
S ta nd ard RF V Va lu es : 1 49 , 63 5, 22 15 ,5 06 1
Calibration method : 7
Calibration(S1) Dose Value : 48.2 mUI/ml
Control C1 Dose Value Range : 5.27 -- 7.37mUI/ml
Calibrator (S1) RFV range : 1858--3318 Max. CV%:10.0
bioMrieux sa 69280 Marcy l'Etoile France
The theoretical calibration
curve is stored in memory
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Calibration When to perform what ?
New Lot of Reagent :1. MLE card2. Adjustment with S1 (or S2)
Every 14 Days :
1. Adjustment with S1 (or S2)
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THE CURRENT VIDAS MENU
Immunochemistry
Infectious diseases
Immunocoagulation
Antigen detection
Industry
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VIDAS : more than 90 parameters
Infectious diseases
HIV : early screening and confirmation: HIV duo ,Ag P24...
Hepatitis diagnosis : HAV IgM, Ag HBs, HBc IgM, anti HBs...
Toxoplasmosis, Rubella , CMV...and confirmation withavidity
Antigen detection : Chlamydia, clostridium difficile toxin A...
Immunochemistry
Fertility : hCG, Estradiol, FSH, LH, Prolactine, Progesteron, Testo..
Thyroid :TSH, T3, T4...
Tumor markers : ACE, AFP, TPSA, CA...
Allergy : IgE, Stallertest, Stallertroph, Stallergy
Immunocoagulation
exclusion of deep venous thrombosis :D Dimer
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VIDAS and mini VIDAS :
2nd immunoassay system
sold in the world