Preparedness for the detection Preparedness for the detection of emerging-and re-emerging of emerging-and re-emerging pathogens in Croatia pathogens in Croatia Alemka Markotić, MD, PhD Associate professor University Hospital for Infectious Diseases Department for Research Department for Cinical Immunology Zagreb Croatia
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Preparedness for the detection of emerging-and re-emerging pathogens in Croatia
Preparedness for the detection of emerging-and re-emerging pathogens in Croatia. Alemka Markotić, MD, PhD Associate professor University Hospital for Infectious Diseases Department for Research Department for Cinical Immunology Zagreb Croatia. History. - PowerPoint PPT Presentation
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Preparedness for the detection of Preparedness for the detection of emerging-and re-emerging pathogens emerging-and re-emerging pathogens
in Croatiain CroatiaAlemka Markotić, MD, PhD
Associate professor
University Hospital for Infectious DiseasesDepartment for Research
Department for Cinical ImmunologyZagrebCroatia
HistoryHistory• The University Hospital for Infectious Diseases was founded in the spring was founded in the spring
of 1893of 1893, under the name of the City Hospital for Contagious DiseasesCity Hospital for Contagious Diseases
Central Diagnostic Laboratory
BSL-3BSL-3
HantavirusesHantaviruses
POZITIVAN
GRANIČAN
NEGATIVAN
Serology – acute case detection and Serology – acute case detection and sero-surveysero-survey
• Puumala and Dobrava viruses
• ELISA (IgM, IgG – reagents provided by CDC)
• POC
Markotić et al. Nature Med 1995Markotić et al. Nature Med 1996Markotić et al. J Med Virol 2002Markotić et al Scand Infect Dis 2002Miletić Medved M et al. Lancet 2002Markotić et al. Croat Med J 2002
BSL2BSL2
Markotić et al. J Med Virol 2002Cvetko et al. J Med Virol 2005Cvetko et al. Am J Trop Med Hyg 2006 Plyusnina A et al. J Med Virol 2011Tadin A et al. VBZD (under review)
Molecular detection – human and Molecular detection – human and rodents samplesrodents samples
BSL2BSL2
Puumala, Dobrava, Tula, Saarema viruses
Markotić A et al. J Gen Virol 2003
Markotić A et al. Coll Antropol 2007
Culture based Culture based techniquestechniques
VeroVero E6MRC-5
293-HEKTHP-1
Prim. monocytes
BSL3BSL3
Merdić E. et al., Infektološki glasnik (Croatian Journal of Infection) 2008; 1: 17-21.Out of 50 mosquito species recorded in Croatia, 15 have potential medical significance. Anopheles atroparvus, An. plumbeus, An. sacharovi, Culex pipiens, and Aedes albopictus are among the most significant species that are potential vectors in Croatia. Climatic changes play an important role in changing the condition of habitats, making it possible for new species to enter certain areas. The tiger mosquito was recorded in Croatia in 2004, and it can now be found in Istria, and more increasingly in Zadar, Šibenik, Split and Dubrovnik, with new sites found on the islands of Cres and Lošinj, which are very attractive tourist destinations.