Preparative isolation and purification of antioxidative stilbene oligomers from Vitis chunganeniss using high-speed counter- current chromatography in stepwise elution mode 2009 10.22 MinSeok Kang Department of Chemistry, Zhejiang University, Hangzhou, China College of Food Science and Biological Engineering, Zhejiang Gengshang University, Hangzhou, China Shan He Yanbin Lu Liyan Jiang Bin Wu Feiying Zhang Yuanjiang Pan Journal of separation science April 30 200
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Preparative isolation and purification of antioxidative stilbene oligomers from Vitis chunganeniss using high-speed counter-current chromatography in stepwise.
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Preparative isolation and purification ofantioxidative stilbene oligomers from Vitis
chunganeniss using high-speed counter-currentchromatography in stepwise elution mode
2009 10.22 MinSeok Kang
Department of Chemistry, Zhejiang University, Hangzhou, ChinaCollege of Food Science and Biological Engineering, Zhejiang Gengshang University, Hangzhou, China
Shan He Yanbin Lu Liyan Jiang Bin Wu Feiying Zhang Yuanjiang Pan
Journal of separation science April 30 2009
Introduction• HSCCC in Stepwise elution mode• Sample profile (Vitis chunganeniss)
Experiment procedure & result• HSCCC• DPPH & Electron paramagnetic resonance
Further study (Application)
KD
=
C upper
C lower
K 0.5 ≤
≤ 1.0(2.0)
Proper range of K value
The distribution constant
Journal of Chromatographic Science, Vol. 47, May/June 2009
Judging K value is not easy work.
Inevitable selection
What is the stepwise elution mode?
Add up two systems
If you are suffer from finding proper solvent system at one step
These peaks are suitable for 5:5:6:4
These peaks are suitable for 1:1:1:1
Sample profile
东南葡萄Vitaceae
Traditionally using for…1. Infectious hepatitis2. Physical injury
No phytochemical or pharmacological investigation has been reported.
Sample Preparation
Dried powder of V. chunganeniss (2.5kg)
MeOH extractionCrude sample
(270g)-> Aqueous
solutionSolvent Fractionation with EA ( 5
times ]
Crude sample (120g)
Evaporated under reduced pressure at 40’C
HSCCC separation
Preparation of two-phase solvent system
Methanol : water gradient [ MeOH 0-40min / 30-70% ]