PRODUCT BULLETIN HUMAN IDENTIFICATION Prep-n-Go™ Buffer What is the Prep-n-Go ™ Buffer? Prep-n-Go ™ Buffer is a best-in- class solution designed to enable high quality direct PCR amplification of non-FTA ® reference samples without the need for heat incubation, extraction or purification. Prep-n-Go ™ Buffer enables increased STR profile quality with fewer intermediate steps than currently available solutions for non-FTA ® reference samples. • Eliminates the requirement for heat incubation or additional workflow steps • Designed to provide consistent, interpretable peaks above the detection threshold and high quality, well- balanced STR profiles • Helps simplify automation requirements and expand throughput capabilities • Helps maximize sample integrity and data quality • Developed according to stringent performance standards to enable higher data reproducibility, reduced artifacts, and increased success rates with Applied Biosystems ® STR direct amplification solutions Why should I choose Prep-n-Go ™ Buffer?
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Prep-n-Go™ Buffer - Applied Biosystems · PRODUCT BULLETIN HUMAN IDENTIFICATION Prep-n-Go™ Buffer What is the Prep-n-Go™ Buffer? Prep-n-Go™ Buffer is a best-in- class solution
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PRODUCT BULLETINHUMAN IDENTIFICATION
Prep-n-Go™ Buffer
What is the Prep-n-Go™ Buffer?
Prep-n-Go™ Buffer is a best-in-class solution designed to enable high quality direct PCR amplification of non-FTA® reference samples without the need for heat incubation, extraction or purification.
Prep-n-Go™ Buffer enables increased STR profile quality with fewer intermediate steps than currently available solutions for non-FTA® reference samples.
• Eliminates the requirement for heat incubation or additional workflow steps
• Designed to provide consistent, interpretable peaks above the detection threshold and high quality, well- balanced STR profiles
• Helps simplify automation requirements and expand throughput capabilities
• Helps maximize sample integrity and data quality
• Developed according to stringent performance standards to enable higher data reproducibility, reduced artifacts, and increased success rates with Applied Biosystems® STR direct amplification solutions
Why should I choose Prep-n-Go™ Buffer?
International Sales For our office locations please call the division headquarters or refer to our Web site at http://www.lifetechnologies.com/about-life-technologies/contact-us.html
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Figure 1. An example of a profile generated from a Bode Buccal DNA Collector™ sample using the Prep-n-Go™ Buffer and the Identifiler® Direct kit.
Figure 2. An example of a profile generated from a Bode Buccal DNA Collector™ sample using the Prep-n-Go™ Buffer and the NGM SElect™ kit.
Frequently Asked Questions
Q. How does Prep-n-Go™ Buffer perform with other sample collection methods or other STR kits?A. Prep-n-Go™ Buffer has been validated internally for direct PCR amplification of reference samples collected with the Bode Buccal DNA Collector™ using the Identifiler® Direct kit. We have demonstrated initial feasibility using the Prep-n-Go™ Buffer with the NGM™ and NGM SElect™ PCR Amplification Kits and samples collected with buccal swabs. More extensive evaluations of these workflows are currently being investigated.
Q. Where can I find detailed information on the Prep-n-Go™ workflow?A. Details on the Prep-n-Go™ workflow are available in the Identifiler® Direct PCR Amplification Kit User Guide (P/N 4415125). If your laboratory is interested in using the Prep-n-Go™ Buffer with the NGM™ and NGM SElect™ kits or for direct amplification of samples collected with buccal swabs, please contact your HID support specialist.
Q. How many reactions can be performed with one kit?A. Prep-n-Go™ Buffer is available in one package size of 1,000 reactions. This packaging solution is designed to enable processing of Bode Buccal DNA Collector™ samples. An additional larger package size designed to enable processing with buccal swabs (which require a larger buffer volume) is in development.
ORDERING INFORMATION
Description Quantity P/N
Prep-n-Go™ Buffer 1,000 tests, 2 μL/test 4467079
For Research, Forensic, or Paternity Use Only. Not intended for any animal or human therapeutic or diagnostic use.
The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Buccal DNA Collector is a trademark of Bode Technology.