Malliga et al, 2015/ Preliminary phytochemical screening and HPTLC fingerprinting profile International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (2) 32- 40 32 ORIGINAL ARTICLE International Research Journal of Pharmaceutical and Biosciences Pri -ISSN: 2394 - 5826 http://www.irjpbs.com e-ISSN: 2394 - 5834 Preliminary Phytochemical Screening and HPTLC Fingerprinting Profile of Leaf Extracts of Moringa oleifera and Phyllanthus emblica. N. Malliga Elangovan 1* , M.S. Dhanarajan 2 , I. Elangovan 3 1 Research Scholar, Department of Biochemistry, Manonmaniam Sundaranar University, Tirunelveli. 2 Principal, Jaya College of Arts and Science, Thirunindravur, Chennai. 3 Biochemist, Meenakshi Mission Hospital, Madurai, Tamil nadu. Article info Abstract Article history: Received 10 March 2015 Accepted 18 March 2015 *Corresponding author: [email protected]Copyright 2015 irjpbs Objective: To establish the physical constants and fingerprint profile of Phyllanthus emblica and Moringa oleifera using high performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done, physical constants were evaluated and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS-1.4.3 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, terpenoids, phenolic compounds, proteins, reducing sugars, fats and oils. HPTLC finger printing of petroleum ether extract of Moringa oleifera leaf powder under white light reveal presence of eight components 0.24, 0.29, 0.34, 0.54, 0.76, 0.83, 0.93, 0.99. Component number 8 at Rf 0.99 showed maximum concentration. Petroleum ether extracts of Phyllanthus emblica leaf powder showed seven peaks with Rf values in the range with their Rf value - 0.26 0.34 0.55, 0.77 0.80, 0.95. Component number 6 at 0.95 Rf value showed maximum concentration. Conclusions: It can be concluded that HPTLC fingerprint analysis of Petroleum ether extract of leaf powder extracts of Phyllanthus emblica (PELPE) and Moringa oleifera (PELMO) can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations. Keywords: Phytochemicals, HPTLC, Petroleum ether extracts, Phyllanthus emblica and Moringa oleifera.
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Malliga et al, 2015/ Preliminary phytochemical screening and HPTLC fingerprinting profile
International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (2) 32- 40 32
ORIGINAL ARTICLE
International Research Journal of Pharmaceutical and Biosciences
Pri -ISSN: 2394 - 5826 http://www.irjpbs.com e-ISSN: 2394 - 5834
Preliminary Phytochemical Screening and HPTLC
Fingerprinting Profile of Leaf Extracts of Moringa oleifera
and Phyllanthus emblica. N. Malliga Elangovan1*, M.S. Dhanarajan 2, I. Elangovan3
1 Research Scholar, Department of Biochemistry, Manonmaniam Sundaranar University, Tirunelveli. 2 Principal, Jaya College of Arts and Science, Thirunindravur, Chennai. 3Biochemist, Meenakshi Mission Hospital, Madurai, Tamil nadu.
Objective: To establish the physical constants and fingerprint profile of Phyllanthus emblica and Moringa oleifera using high performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done, physical constants were evaluated and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS-1.4.3 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, terpenoids, phenolic compounds, proteins, reducing sugars, fats and oils. HPTLC finger printing of petroleum ether extract of Moringa oleifera leaf powder under white light reveal presence of eight components 0.24, 0.29, 0.34, 0.54, 0.76, 0.83, 0.93, 0.99. Component number 8 at Rf 0.99 showed maximum concentration. Petroleum ether extracts of Phyllanthus emblica leaf powder showed seven peaks with Rf values in the range with their Rf value - 0.26 0.34 0.55, 0.77 0.80, 0.95. Component number 6 at 0.95 Rf value showed maximum concentration. Conclusions: It can be concluded that HPTLC fingerprint analysis of Petroleum ether extract of leaf powder extracts of Phyllanthus emblica (PELPE) and Moringa oleifera (PELMO) can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.