Practical protocol 1 and 2 Differentation of bacteria 1. Preparation of Gram-stained smear Prepare slide and apply Gram-staining according to teachers instructions. Note the result: What characteristic features You can observe? 2. Ready smears. Observe and fill the gaps below. a/ Congo-red staining What You can observe?..................................... b/ Ziehl-Neelsen staining Application of staining……………………….. c/ Methylene blue staining (Loeffler) Application of staning………………………. 3. Examination of hemolysis on sheep-blood agar Describe the type of hemolysis and give an example of bacterial species name Alpha………………………………….species………………………………….. Beta…………………………………...species………………………………….. Gamma………………………………. species………………………………….. 4. Catalase test Note the result (presence/absence) for: Staphylococci…………………………………….. Streptococci……………………………………... Enterococci………………………………………
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Practical protocol 1 and 2 Differentation of bacteria · Practical protocol 1 and 2 Differentation of bacteria 1. Preparation of Gram-stained smear Prepare slide and apply Gram-staining
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Practical protocol 1 and 2
Differentation of bacteria
1. Preparation of Gram-stained smear
Prepare slide and apply Gram-staining according to teachers instructions. Note the result:
What characteristic features You can observe?
2. Ready smears. Observe and fill the gaps below.
a/ Congo-red staining
What You can observe?.....................................
b/ Ziehl-Neelsen staining
Application of staining………………………..
c/ Methylene blue staining (Loeffler)
Application of staning……………………….
3. Examination of hemolysis on sheep-blood agar
Describe the type of hemolysis and give an example of bacterial species name
Alpha………………………………….species…………………………………..
Beta…………………………………...species…………………………………..
Gamma………………………………. species…………………………………..
4. Catalase test
Note the result (presence/absence) for:
Staphylococci……………………………………..
Streptococci……………………………………...
Enterococci………………………………………
5. Examination of bacterial growth on differential and selective media:
6. Characterize culture media
7. Examination of growth requirements
Match the following bacteria: strict aerobic, facultative anaerobic, strict anaerobic with relevant arrows
8. Describe the application of following device
Candle jar- what type of bacteria may be cultured………………………………………………………
Anaerobic Gas-Pak jar – what type of bacteria may be cultured………………………………………..
9. Demonstration:
Determination of bacterial species using biochemical tests (API tests (visual reading), VITEK 2 Compact identification
cards)
Practical protocol 3
Differentation of fungi
1. Preparation of Gram –stained direct smear from oral cavity. Search for fungal cells. Note the result………………..
2. Preparation/observation of Gram –stained smears of different species of Candida cultured on Sabouraud medium.
Answer: are there any significant differences between fungal cells of each species?.......................................... ..........
3. Examination of direct Gram –stained smears from vaginal candidiasis (as an example).
Answer: when and how we can determine the yeast infection basing on the microscopic observation of smear?
………………………………………………………………………………………………………………
4. Yeasts – fill the table below
5. Examination of Candida albicans and non - Candida species in germ- tube test.
Note the result for:
Candida albicans - ………………………………………
Non-albicans Candida species-………………………….
Application of test………………………………………………………………………………………………
Positive result of germ-tube test
6. Molds (on Aspergillus sp. example) – fill the table below
7. Observation of hypha/mycelium of molds and dermatophytes in lactophenol cotton blue slide mounts.
Answer: What is the diagnostic role of slide mounts in identification of filamentous fungi?..........................................
……………………………………………………………………………..
8. Dermatophytes
Observe the growth characteristics of 2 different species of dermatophytes. Note Your observations.