PPB - High Hydrostatic Pressure Induced Mutation

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Presentasi Mata Kuliah Pemuliaan Tanaman Paraseksual – PNB 665

MUTASI TERINDUKSI TEKANAN

HIDROSTATIK TINI Oleh:

Adhityo Wicaksono (13/354311/PPN/3833)

Purwito !oko "uwono (13/353##$/PPN/38#4)

 %ika &ah'a "unita (13/3434#/PPN/3$8)

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han*+ W,+ -, .iu+ , hen*+ 0, en*+ , Wu+ 2A%,

da 0ila+ 2, uan (#13), nduction o rice'utations 6y hi*h hydrostatic 7ressure, PlantPhysiolo*y and ioche'istry $#: 18918$, O:1#,1#1/!,7la7hy,#13,#5,#34

 2urnal Acuan ;ta'a

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<uta*enesis

=araonensional (&adiasi+

ki'iawi)

<encaricara 6aruyan* le6ih

a'an

Pen**unaan tekananhidrostatik

se6a*ai

'uta*en>sik

Pendahuluan

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∗ Pe'anaatan tekanan hidrostatik yan*

di6erikan secara 'ekanik den*an 6antuan7o'7a 7ada sa'7el

∗ 0a'7el kali ini: i!i 7adi (han*+ et al,+ #13)

Prinsi7 asar

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Persia7an %ana'an

• i!i 7adi arietas "ueen*?han disia7kan 7ada air akuades steril• Alat disia7kan

Perlakuan@

0creenin*

• Pe'6erian tekanan den*an 7o'7a (kontrol 7ada tekananat'oser)

• i!i ditana' 7ada ladan* u!i+ 395 6i!i dia'6il untuk 7enana'an<

• 1### 6i!i dia'6il dari tana'an < untuk analisis

Analisisasil

• Persia7an inti dari e'6rio 6i!i• =o'et assay

Penelitian

(han*+ et al,+ #13)

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∗ Oryza sativa ss7, indica c, B"ueen*?hanC

 2enis 7adi yan* di*unakan

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Alat

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∗ 0a'7el 7adi se6anyak ## * dike'as dan

diisi air distilasi steril se6anyak $ D 1#93 . dandiletakkan dala' ta6un*

∗ etika tuas ditekan+ tekanan 6erta'6ah danskala 6isa di6aca 7ada 6aro'eter alat

Prinsi7 Alat @ Pen**unaan

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K!ntr!l Perlakuan

#,1 <7a

(%ekananat'os>rnor'al)

5 <Pa 5# <Pa $5 <Pa 1## <Pa

Earia6el Perlakuan

Pe'6erian tekanan dilakukan sela'a 1 !a',

 %ekanan

Perlakuan

4 !a' # !a' 3 !a'

Perenda'an awal untuk < ( pre-soaking)

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 "enis Mutan #an$ Dihasilkan

erdil (Dwarf )

atan* Pan!an* (Long stem) %unas sedikit (Few tillers)

 %unas 7osisi tin**i (High position tillers)

Fksersi 7anikula tak se'7urna (Incomplete panicle exertion)

.a'6at dewasa (Late mature)<ayan* 7an!an* (Long awns)

ulit 6i!i coklat (rown husks)

asil

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asil

eteran*an: =% G ontrol+ < 9 <utan

<utan den*an tunas lateral 6anyak (=%: 14+ <: 5#)<utan den*an internodus yan* le6ih 7an!an* (< le6ih 7an!an* 3 c' dari<utan kerdil (=%: 5 c'+ <: 35 c')<utan yan* 6er6un*a le6ih ce7at (=%: 8H hari+ <: $H hari)<utan den*an 'alai le6ih 7an!an* (=%: #,5 c'+ <: 94 c')<utan den*an daun le6ih 7an!an* (=%: 1 c'+ <: 4# c')<utan den*an 6i!ile6ih 6esar<utan den*an warna 6atan* un*u (=%: hi!au)<utan den*an kulit 6i!i 6erwarna coklat (=%: kunin*)

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asil

 %a6el: a'7ak la'a 7erenda'an awal terhada7 ke'unculan 'utan7ada <

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asil

 %a6el: Pen*aruh tekanan 7ada 'uta*enesis <1 terhada7 ke'unculan 'utan7ada <

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Analisis asil

asil !omet assay  untuk 'elihat kerusakan

NA 7ada hasil 7e'6erian tekananhidrostatik,

asil !omet assay , erkas disa'7in* lin*karan sa'7eladalah tail yan* 'eru7akan 6anyaknya NA yan* rusak

aki6at 7e'6erian tekanan hidrostatik

asil !omet assay  6erkala untuk 'elihat ke'a'7uan7er6aikan NA 7ada 'utan

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∗ <udah

∗ <urah

∗ A'an

Nilai .e6ih <uta*enesis en*an %ekanan idrostatik

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∗ '7roisasi untuk 7en*u!ian le6ih 6era*a'

∗ '7roisasi untuk 7roduksi le6ih 6anyak

∗ Auto'asi alat

Pros7ek

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en*an se'akin 'a!unya teknolo*i+

dihara7kan 'etode ini 6isa di*unakan dala'skala luas+ den*an hasil le6ih 6anyak dan

7en**unaan alat den*an skala le6ih akuratuntuk hasil le6ih 6era*a',

Penutu7

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