Poultry show manila 2010

PHYTOCONSTITUENTS IN ANIMAL FEED

AN OPPORTUNITY

Dr (Capt) Tanweer AlamDr (Capt) Tanweer Alam

INDIA

Global trend

Global Feed additive market to grow to $18 billion by 2014

Largest contributor is the antibiotic/ anti bacterial (28%) and fastest growing is amino acid segment (6%)

Herbal/ Phytoconstituents based products market is catching up in an impressive manner (20% CAGR)

Foodceo.com

Total number of research papers listed on PubMed containing the word "phytoconstituents."

How the usage of Phytoconstituents based products have progressed ?

The Transition…..

Ayurvedic Era

Herbal Era

Phytochemical Era

Ethno-medical information

Development of prototype formulations

Validation of safety & efficacy using invivo models

Chemical fingerprintingMarketing

Conventional Approach of Conventional Approach of Phytoconstituent based product Phytoconstituent based product development:development:

Ethno-medical information

Selection and standardization of in

vitro assays

Validation of safety and efficacy using in

vivo models

International marketing

Screening program-Select plants-Select plant parts-Select solvent

Bioactivity guided fractionation

Development of prototype formulation

(Based on compatibility studies)

Standardization and Chemical fingerprinting

Modern Approach of Phytoconstituent Modern Approach of Phytoconstituent based product development:based product development:

Chemical Constituents

Of Herbs

Primary MetabolitesCarbohydrate Fat

Protein

Secondary MetabolitesAlkaloids

TurpenoidsFlavonoids

Others

Secondary Metabolites are:

Unique to each plant & have therapeutic value

What’s the need?What’s the need?

Secondary Metabolites

Part used

Geographical factor

Period, time & method of collection

Storage

Water availability

Drying

Packing and Transportation

Age of the plant

Factors affecting Sec Metabolites?Factors affecting Sec Metabolites?

•A - SYNERGISM i.e., 2+ 2 = > 4

•B - ADDITIVITY i.e., 2 + 2 = 4

•C - ANTAGONISM i.e., 2 + 2 = < 4

B

Incr

easi

ng

Con

cen

trat

ion

Of

Her

bal

Ext

ract

- Y

Increasing ConcentrationOf Herbal Extract - X

A

C

Developing quality Phytoconstituent Developing quality Phytoconstituent formulationformulation

Reasons why Phytoconstituent based Reasons why Phytoconstituent based products are usage of choice across the products are usage of choice across the worldworld

Growing credentials with validated results Cost economy High safety margin with no resistance

development tendency

Growing credentials:Herbal Monographs

Publication and availability of authoritative monographs by various organizations, improving the scientific credibility of herbs.

Indian HerbalPharmacopoeia

British Herbal Pharmacopoeia

English Translation of

German Commissions

E monographs

American Herbal Pharmacopoeia

( AHP )

World Health Organization

(WHO)

Europe Scientific Cooperative

on Phytotherapy (ESCOP)

United States Pharmacopoeia

( USP )

Confluence of Herbal

Pharmacopoeia

Cost Economy

Comparatively Less Gestation time of phytodiscovery

Pharmaceutical Research and Development (R & D) Process

DefinitionsIND = Investigational New Drug Application FDA = U.S. Food and Drug Administration NDA = New Drug Application Source: www.Innovation.org

Evolving into a composite phytochemical based pharmaceutical major with presence in

Animal Health Products

Standardized Herbal Extracts

Human Health Supplements

Phytochemical Reference Standards

INDIAINDIA

Our AccreditationsOur Accreditations R&D centre also holds reputed accreditations from

Department of Science & Technology, Government of India.

The research team has more than one dozen patents and a few international patents on its herbal products.

Contribution to USP and Indian Government Pharmacopoeia

Our AccreditationsOur Accreditations

Screening of biological activity for new usage

Our predicted direction of herbal research

Role of Phytoconstituents

As Performance Enhancer

Andrographis paniculata

CH3 CH2OHH

CH3

O

OOH

CH2

OH

Molecular Form ula = C 20 H 30 O 5

Form ula W eight = 350.449

Phyllanthus amarus

Marker constituents: Phyllanthin &

Hypophyllanthin

OCH3

H

OCH3

HH3CO

H3CO

OCH3

OCH3

Molecular Form ula = C 24 H 34 O 6

Form ula W eight = 418.523

O

O

H3CO

OCH3

OCH3

OCH3

OCH3

H

H H

Molecular Form ula = C 24 H 30 O 7

Form ula W eight = 430.491

GLOBAL QUALITY TESTED & TRUSTED

India

Malaysia

Japan

USA

& Italy

Country - Malaysia

Field trial in commercial broilers (AA) – Dr.V.Raghavan, Seng Heng Chan Feed

Country - Japan

Field trial in commercial broilers (Chanky) – Kohara Feed & Breeding Co. Ltd. Poultry Farm, Wakayama

Country - USA

Field trial in commercial broilers – Dr. Danny Hooge, Utah State University, Logan –UT

Country - Italy

Field trial in commercial broilers - Dr.Schienoni Milano

Source : World Poultry - V.15 # 11, 1999

MYCOTOXINS - A WORLDWIDE SCENARIO

“Food and Agriculture Organisation (FAO) of the UN estimated

that 25% of the world's grain supply is contaminated with

Mycotoxins ”.

Liver in a good condition

The target organ for the mycotoxins and attack of other chemical toxicants is LIVER.

Liver in a good functional condition is very useful for basic detoxification mechanism and overall digestion & metabolic functions of the body.

Zigbir has potent effect on the development & efficient functioning of liver under normal and challenged condition.

MYCOTOXINS

HEPATOPROTECTION (Against FUNGAL TOXINS)

Prof.D.Narahari,Ph.D., Prof. & Head, Poultry Science, Vety.College, Chennai

HEPATOPROTECTION (Against FUNGAL TOXINS)

GROSS LESION - LIVER(e) ND H.I Titers

BETTER FEED & FEEDING

Gross pathology of liver of challenged untreated group (A) reveals hepatomegaly, severe discoloration, blunt edges and fragility.

While the gross pathology of liver of the challenged treated group (B)

is closer to the normal.

HEPATO PROTECTION (Against CHEMICAL TOXICANTS)

Dr.Deben Sapcota Associate Professor Dept. of Poultry Science Assam Agricultural University

Utilization of Xanthophill

2.3

4.5

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

CONTROL TREATED

[a] Skin Colour (Grade)

2.3

4.6

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

CONTROL TREATED

[b] Shank Colour (Grade)

Roche colour fan index basis

• In broilers, to growth, weight gain, FCR livability

• Enhance egg production ECR & minimise thin shell and egg breakage due to liver related problems

•To maintain proper digestion, absorption, general health and, feed intake

• To enhance hepatic functions

• To protect the birds from aflatoxins and other feed related problems

Which Spells Quality

Usage

• 300 gms per ton of feed

• Depending on the quality of feed ingredients and atmospheric moisture content, the dose may be increased upto 400 gms per tin of feed or as per the advice of the nutritionist.

Broilers & Layers : Form day l continuously, till marketing.

Which Spells Quality

25 kg multi layer bag.

During high risk period

Recommendation

Presentation

Mixing rate

Role of Phytoconstituents

To replace bioactivity of Vitamin C

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Emblica officinalis

Part used : Dried FruitsMain Uses : Immunomodulatory and Antioxidant (Suresh & Vasudevan, 1994).Active principle : Gallic acid

Gallic acid

Ursolic Acid

Part used : Whole plantMain Uses : Immunomodulatory, Antistress (Sen et.al.,1992)Active principle:Ursolic acid

Ocimum sanctum

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Emblica officinalis

Gallic acidChebulic acidEllagic acidChebulinic acid

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Known to be active in scavenging DPPH*, Superoxide anion

*DPPH: diphenyl picrylhydrazyl (free radicals), turns colorless when reduced by antioxidant.

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Chebulinic acid is shown to be active in DPPH and methyl linoleate oxidation models with 93 and 34 % inhibitory activity respectively in these models.

A total of 13 hydroxy groups representing chebulinic acid and is believed to be responsible for its powerful anti-oxidant activity (Klika KD et al. 2004).

L-Ascorbic acid

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Potent Anti-oxidant

Under stress free condition Phytocee-Under stressful condition

A.T.Ps. Energy

Free Radicals Scavenged S.O.D.

More energy More A.T.Ps.

Extra Free Radicals (> normal)

Destroyed by AntioxidantsDestroyed by Antioxidants

The phytoactives of Phytocee possess a potent ability todonate H+ atom andacts as a naturalanti-oxidant.

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Phytocee shows a potent free radical scavenging activity very similar to L-ascorbic acid(Vitamin 'C').

SCIENTIFIC VALIDATION: Anti-oxidant Activity

Principle: DPPH [1,1-diphenyl-2-picryl hydrazyl] is a stable free radical with purple colour, the intensity of which is measured at 510nm spectrophotometrically. Antioxidants reduces DPPH to 1,1-diphenyl-2-picryl hydrazine, a colourless compound. 1, 1-diphenyl-2-picryl hydrazyl

Purple colour1, 1-diphenyl-2-picryl hydrazine

Colourless

Effect of L-Ascorbic acid & Phytocee on free scavenging activity

Phytocee L Ascorbic acid

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Oxidative stress (OS) is one among the common stressors of cells. OS can result in membrane damage leading to several pathological effect.

Reference: Chandarasekaran C.V., et al, Invitro efficacy

and safety of poly-herbal formulations. Toxicol,

invitro (2010), doi:10, 1016/j.tiv.2009, 11, 021.

As per various researchPhytocee exhibits anappreciable cellularanti-oxidant effect.

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Phytoactives of Phytocee Possess a proven immuno-modulating property againstbacterial as well as viral infections

An Immunomodulator

ConclusionThe results indicate that, both the products i.e., Phytocee and Vitamin-C (L-Ascorbic acid) possess the same macrophage phagocytosis stimulating activity. Therefore, Phytocee can replace Vitamin-C cent percent and restores the immune status of the bird under any stress or infective conditions.

Effect of Phytocee and Vitamin 'C' on macrophage phagocytosis

Phytocee / Vit. C (µg/ml)

% Infected macrophages

0

100

500

83 ± 4

100 ± 0

100 ± 4

-

1.2

1.2

-

1.1

1.2

83 ± 4

96 ± 1

100 ± 1

Phytocee Vit. C Phytocee Vit. C

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

• The phytoactives of Phytocee are very stable at high thermal temperature under moist & dry heat conditions.

• Possesses long lasting activity when stored under normal room temperature.

Thermostability

TRACK C1: Phytocee Control sample without heat treatmentTRACK T1: Phytocee Heated at 1050C for 30 minutesTRACK T2: Phytocee Heated at 1050C for 1 hourTRACK T3: Phytocee Heated at 1050C for 2 hours

Laboratory Test Report Sample heated at 1050 C for 30 minutes, 1 hour and 2 hours

254nm 366nm Visible Visible after

derevitisation

Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C

Usage

To optimize:• Body resistance, thereby livability

• Egg production and shell quality

• Fertility and hatchability in breeders

• Overall performance of birds

Mixing Rate

1:1 replacement of synthetic Vitamin C or as directed by the nutritionist

1 kg and 5 kg

Availability

More Phytoconstituents options

For Replacement of Synthetic Methionine & Equivalent