Sensors and Biosensors group A.P.F. Turner Aristotele University of Thessaloniki (Greece) Bucharest University (Romania) Cranfield University (UK) Brno University (Cezch Republic) Cluj-Napoca University (Romania) •Post-docs Francesca Bettazzi Simona Scarano •PhD students Andrea Ravalli Diego Voccia Stefano Mariani •Visiting professor •Erasmus link Giovanna Marrazza Ilaria Palchetti Maria Minunni Department of Analytical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Iuliu Hatieganu”, Cluj-Napoca, Romania Cecilia Cristea, Robert Săndulescu Nanobioelectronics & Biosensors Group, Catalan Institute of Nanotechnology, Campus, Bellaterra, Barcelona, Spain Alfredo de la Escosura-Muñiz, Arben Merkoçi Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran Mohammad Mazloum-Ardakania Department of Analytical Chemistry, São Paulo State University (UNESP), Araraquara/SP, Brazil Hideko Yamanaka Cranfield Health, Università di Cranfield, Regno Unito Sergey A. Piletsky Dipartimento di Chimica e Fisica, Università di Brescia Guido Faglia Dipartimento di Chimica e Fisica, Università dell'Aquila Luca Lozzi Department of Physical and Analytical Chemistry, University of Oviedo, Spain Maria Jesus Lobo Castañón Department of Physical and Environmental Science, University of Toronto at Scarborough, Toronto, Canada Kagan Kerman Università “La Sapienza” and Scientific Director, Laboratorio Antidoping, FMSI, Roma Francesco Botrè Istituto di Biochimica delle Proteine, CNR Giuseppe Manco Collaborations 1. Development and evaluation of sensors and biosensors based on the coupling of electrochemical, optical and piezoelectric sensors with enzymes, antibodies, bacteria, whole tissues and nucleic acids 2. Solving analytical problems in clinical chemistry, experimental medicine, food chemistry and environmental analysis 3. Development of new immobilization chemistries of biomolecules (enzymes, proteins, nucleic acids, etc.) and analytical procedures suitable for use with biosensor devices, i.e. flow injection analysis, flow systems, and microdialysis Group’s expertise Autolab Potentiostat/Galvanostat (Metrohm, NL) Palm Sens electrochemical sensor interfaces (NL) Home made screen-printed electrodes Electrochemical instrumentations Other instrumentations • Nanodispensing systems • Spin coater • Optical microscope • Themocyclers Biorad for PCR • Electrophoresis apparatus • Spectrofluorimeter • Spectrophotometer • Fiber optic spectrophotometer-Ocean optic • ELISA plates reader • Cary 100 Bio – UV/Vis spectrometer 1. Diaconu, I., Cristea, Hârceagǎ, V., Marrazza, G., Berindan-Neagoe, I., Sǎndulescu, R. Electrochemical immunosensors in breast and ovarian cancer. Clinica Chimica Acta 425, 2013, 128-138. 2. Taleat, Z.; Ravalli, A.; Mazloum-Ardakani, M.; Marrazza, G., CA125 Immunosensor Based on Poly- Anthranilic Acid Modified Screen-Printed Electrodes. Electroanalysis 2013, 25 (1), 269-277. 3. Ilkhani, H.; Arvand, M.; Ganjali, M. R.; Marrazza, G.; Mascini, M., Nanostructured Screen Printed Graphite Electrode for the Development of a Novel Electrochemical Genosensor. Electroanalysis 2013, 25 (2), 507-514. 4. Bettazzi, F., Enayati, L., Sánchez, I.C., Motaghed, R., Mascini, M., Palchetti, I., Electrochemical bioassay for the detection of TNF-α using magnetic beads and disposable screen-printed array of electrodes. Bioanalysis 2013, 5 (1), 11-19. 5. Bettazzi, F., Hamid-Asl, E., Esposito, C.L., Quintavalle, C.,Formisano N.,Laschi, S.,Catuogno, S.,Iaboni, M., Marrazza, G., Mascini, M.,Cerchia, L.,De Franciscis, V.,Condorelli, G.,Palchetti, I., Electrochemical detection of miRNA-222 by use of a magnetic bead-based bioassay. Analytical and Bioanalytical Chemistry 2013, 405, 1025-34. 6. Scarano, S.; Vestri, A.; Ermini, M. L.; Minunni, M., SPR detection of human hepcidin-25: A critical approach by immuno- and biomimetic-based biosensing. Biosensors and Bioelectronics 2013, 40 (1), 135-140. 7. Ermini, M. L.; Mariani, S.; Scarano, S.; Minunni, M., Direct detection of genomic DNA by surface plasmon resonance imaging: An optimized approach. Biosensors and Bioelectronics 2013, 40 (1), 193-199. 8. Ravalli, A.; dos Santos, G. P.; Ferroni, M.; Faglia, G.; Yamanaka, H.; Marrazza, G., New label free CA125 detection based on gold nanostructured screen-printed electrode. Sensors and Actuators, B: Chemical 2012. 9. Ilkhani, H.; Mascini, M.; Marrazza, G., The potential affibodies in new cancer marker immunosensors. 2012; Vol. 109 LNEE, 15-18. 10. Hamidi-Asl, E.; Palchetti, I.; Mascini, M. Introduction of an electrochemical genosensor for detection of p53 gene via sandwich hybridization method , 2012, Vol. 109 LNEE , pp. 37-41 11. Al-Khafaji, Q. A. M.; Harris, M.; Tombelli, S.; Laschi, S.; Turner, A. P. F.; Mascini, M.; Marrazza, G., An Electrochemical Immunoassay for HER2 Detection. Electroanalysis 2012, 24 (4), 735-742. 12. Zani, A.; Laschi, S.; Mascini, M.; Marrazza, G., A new electrochemical multiplexed assay for PSA cancer marker detection. Electroanalysis 2011, 23 (1), 91-99. 13. Scarano, S.,Ermini, M. L., Spiriti, M. M., Mascini, M., Bogani, P., Minunni, M., Simultaneous detection of transgenic DNA by Surface Plasmon Resonance imaging with potential application to gene doping detection. Analytical Chemistry 2011, 83 (16), 6245-6253. 14. Ermini, M.L., Scarano, S., Bini, R., Banchelli, M., Berti, D., Mascini, M., Minunni, M., A rational approach in probe design for nucleic acid-based biosensing. Biosensors and Biolectronics , 2011, 26 (11), 4785-4790. 15. Scarano, S.; Scuffi, C.; Mascini, M.; Minunni, M., Surface Plasmon Resonance imaging-based sensing for anti-bovine immunoglobulins detection in human milk and serum. Anal Chim Acta 2011, 707 (1– 2), 178-183. Selected pubblications on clinical diagnostic (2011 – 2013) Other applications and main research topics Optical instrumentations Surface Plasmon Resonance SPR-based instrumentation: - Fully automated Biacore X™ (GE Healthcare) - SPR-imaging (Horiba Scientific- France) Piezoelectric instrumentations (QCM) • 9.5 MHz AT-Cut quartz crystal (14mm); Gold evaporated (42.6mm2 area) on both sides - Seiko (Japan) http://www.bioinspired-nano.eu/en/ •COST ACTION COST TD 1003