Possible factors affecting ribosomal protein expression 1. Autogenous regulation of protein expression. 2. mRNA protein degradation. 3. mRNA secondary structure. 4. Missing factors in E.coli S-30 extract
Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure.
Dec 20, 2015
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Possible factors affecting ribosomal protein expression
1. Autogenous regulation of protein expression.
2. mRNA protein degradation.
3. mRNA secondary structure.
4. Missing factors in E.coli S-30 extract
100
75
50
37
25
15
10
S1
S2
S4
S5
S13?S16?S?
“Specialized” ribosome
GFP X W X W
Ribosome N N S N
AGUGTAUUCACAUA
Future Steps and Issues
1. Increase translation efficiency.
2. Reconstitute 30S and 50S subunits.
3. Genome organization.
4. Autogenous regulation.
4. RNA processing & modifications.
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