Polymerase Chain Reaction By: Stephen Feagles
Polymerase Chain Reaction
By: Stephen Feagles
What is PCR?the automated process that allows a specific
DNA chain also called primers to be replicated over and over again to produce more copies of that DNA
Who Invented it? Dr. Kary Banks Mullisborn on December 28, 1944Ph.D. degree in biochemistry from the
University of California, BerkeleyWon Nobel Prize in Chemistry for PCR
Thermocycler This machines purpose is to alter the
temperature so that the primers can be heated and cooled to perform the processes correctly
Steps of PCR1. Denature 2. Annealing 3. Extension
DenatureHeating up DNA Splitting into two strands 94 degrees Celsius
Annealing Cooling DownAllowing to be bonded 54 degrees Celsius
Extension Taq Enzyme creating copy of original DNATwo new double stranded DNA formed 72 degrees Celsius
Process of PCR (video) http://www.youtube.com/watch?v=eEcy9k_Ks
DI
PCR Importance Creates more DNA
More tests run on specific DNAMore DNA can be tested by different people
ForensicsAllows small amount of DNA to be made into a
lot Research
Mutated DNA can be reproduced and be tested different ways
Bibliography http://vadlo.com/Research_Cartoons/http://
www.youtube.com/watch?v=eEcy9k_KsDIhttp://
flickrhivemind.net/Tags/lab,pcr/Interestinghttp://
www.sivb.org/InVitroReport/IVRs/38-1/karyMullis.asp
http://www.btci.org/k12/bft/PCR/pcr_geneticscreening.html