Polyinform_recultivation_english

Address: Ligovsky pr., 254, St. Petersburg, Russia, 191119E-mail: [email protected]: http://www.polyinform.ru

Tel.: (+7-812) 458-85-73 Tel.: (+7-812) 458-85-74Fax: (+7-812) 458-85-76

Decontamination of soil, ground water and water surfaces

according biotechnology SOILEX®®

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HISTORY1989 JSC POLYINFORM was established. The main activities were Investigation of open pits of building materials,

carrying out engineering-geological and hydro-geological researches for a substantiation of ecologically pure construction of industrial and civil objects.

1992 Establishing of the R&D Department and microbiological lab. Research in the field of biological treatment and remediation of soil and water.

1996 The complex biotechnology SOILEX® was developed for cleaning of oil contaminated soil, water and water surface. The technical solution which were the essence of the technology (the method of working, biopreparation producing, the bio-products themselves) were protected by 16 patents of Russia.

1998 Entering the foreign market. The works on recultivation oil polluted soil at Klaipeda petroleum terminal, Lithuania were carried out as well as clearing of soil, polluted by mineral oil, polyaromatic connections and heavy metals on objects for NKK CORPORATION, Japan.

2003 We received USAID (USA) grant as the winner of competition "Researches and development in priority directions of development of a science and techniques". We won the competition "Research and organizational support of large venture projects" in the frames of Federal Target Scientific and Technical Program "R&D in priority areas of science and technology in 2002-2006”.

2008 Designing and construction of the polygons and special yards for processing of oil sludge at the objects of fuel-gas complex.

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SCIENTIFIC AND TECHNICAL BASE

Highly qualified staff

7 doctors of sciences

The team of more than 40 professionals the high education level of whom allows solving any problem

Technical base

Chemical and microbiological labs

The production line for producing a biological product

Equipment including pumps for oil and oil products gathering (skimmers), hydrodynamic techniques for the tanks cleaning, cleaning the outer water pipes and mini technique

Special site for oil waste processing

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SOILEX® BIOTECHNOLOGYIn the basis of the technology are:

SPECIFIC ASSOCIATION MICROBES-DESTRUCTORS, EFFECTIVE FOR A WIDE SPECTRUM OF POLLUTANTS

The technology includes the following activities:mechanical, agro-chemical, sorption, biological.

POLYINFORM owns one of the largest private collection of the microorganisms of the environmental safety target, including more than 100cultures, selected from the contaminated places located in different climatic zones.

The collection is registered in World Data Centre for Microorganisms WFCCWDCM 772 Japan, 28.01.98

The applied technology received the positive conclusion of the Natural Recourses Ministry of Russia.

The technical solutions which are the essence of the technology are protected by

16 patents of Russia

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PRINCIPLE OF BIOPREPARATION ACTIVITY

H

CCC

CC

C

H

H

H

H H

CC H

H

H

H H

H

O2 + BIO-PREPARATION

CO2

H2O

Bacterial strains of the biological product used hydrocarbons and heavy fractions of petroleum as an energy source for their livelihood.

During the first day after introduction of a biological product microorganisms are activated, there is an increase of their amount and an irreversible process of decomposition of petroleum products to environmentally safe non-toxic products of bacterial metabolism begins.

After the decomposition of mineral oil the mass of micro-organisms not provided with nutrient die off, being a food for intensifying indigenous microflora.

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GENERAL CHARACTERISTIC OF SOILEX® BIOPREPARATION

The basis of SOILEX® biopreparation are aerobian hydrocarbons oxidizing non-pathogenic bacteria sorbed on the ecologically clean inert support.

Constituent microbial association possesses high activity relative to some hydrocar-bons(crude oil, diesel fuel, technical oils, heavy oil and mazut fractions) in the wide pH (4,5-8,2) range.

SOILEX® biopreparations includes vegetative bacteria’ cells related to mesophilic bacterium (maximum activity under high positive temperature 10-40С) and psychro-philic organisms (maximum activity under low positive temperature 3-10С). This provides wide temperature range of biopreparation activity. Also biopreparation in-cludes nutrient medium debris, biogenic salts and inert support.

The titer of viable microorganisms cells not less then 1 x 1010 cells in 1 g of biopre-parations.

Visually the preparation is friable powder of dark-brown color without characteristic odor.

The preparation is non-toxic, has no carcinogenic and cumulative effect, fire and ex-plosive safe, and belongs to IV hazard class.

The preparation must be stored in hermetic plastic sachet under the temperature from +1 to +20C. That provides saving of the titer of viable cells.

The warranty working life of the dry form of SOILEX® biopreparation is one year.

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PRODUCING OF SOILEX® BIOPREPARATION

The inoculum II receiving by mixing

nutrient medium19/6

nutrient medium № 7

nutrient medium

nutrient medium

The inoculum I receiving in 10 tubes

Microorganisms -destructors of hydrocarbons

The inoculum III receiving in the apparates

Fermentation

SOILEX® BIOPREPARATION

V ≤ 5 l.

V = 180 l

V = 4,5 m³.

Producing of a biological product takes place on site from seed: specially selected active microbial associations from our own collection and isolated from the occurrence of pollution that are optimal for this type of pollution and natural-climatic zones.

As a result:

- Repeatedly increases the bio-purification efficiency,

- Independence of the biological products suppliers as well as of the quality of supplied materials

The technological scheme

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SOILEX® biotechnology is efficient for wide spectrum of contaminates

PURIFICATION EFFICIENCY OF SOILEX® BIOTECHNOLOGY

Salts of heavy metals:

Cooper - 60 %Zinc - 60 %Lead - 50 %Arsenic - 50 %Cadmium - 30 %

Organic contamination:

Phenol – 90 % Creosote – 80 %Chlorbenzene - 80%

Oil contamination:

Black oil – 95 % Technical oil – 97 %Benzine – 98 %

Contamination with black oil - control sample, 2 months after the contamination

Contamination with black oil - test sample, 2 months after the beginning of the

treatment

9

16,0

12

34

56

7

Р1

Р2

Р3

Р40

2

4

6

8

10

12

14

16

oil products concentration, g/kg

days

Benzine

Diesel fuel

technical oil

Black oil1,5

0,50,2

0,15 00

00,150,18

5,0

3,0

1,53,0

0,7

12,0

8,0

4,0

2,51,8

1,00,5

8,0

6,0

3,02,0

1,00,5

The dynamics of reduction of various types of petroleum products in soil and ground water

Initial concentration 30 days 60 days 90 days

120 days 180 days 240 days

After 3 months the treatment efficiency for oil-contaminated soil andgroundwater is 80-90%.

EFFICIENCY OF SOILEX® BIOTECHNOLOGY

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Short time necessary for decontamination (3-8 months);

Absolutely environmental friendly technology;

High destructive activity in the wide pH (4,5-8,5) and temperature range (3 – 40 С);

Ability to remove heavy metals including mercury as well as nitrates;

Adaptability to specific needs;

Full rehabilitation of the environment at the contaminated territory

ADVANTAGES OF SOILEX® BIOTECHNOLOGY:

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SOILEX® BIOTECHNOLOGY

Handing the object to the customer (independent expertise)

VI. CHEMICAL AND MICROBIOLOGICAL CONSTANT CONTROL

Treatment of the contaminated ecological systems with SOILEX® accomplished with the agrotechnical activities

V. BIOLOGICAL METHODS

Gathering oil from the surface of water, snow and soil

IV. SORPTION METHODS

Construction of the polygons for oil waste processingExcavation of oil-contaminated soil, pumping of free oil, the removal of vegetation, placement it at special polygons

III. MECHANICAL TREATMENT METHODS

By sorbents and borders at the soilBy booms, oil catchers and filters at the water surfaces

II. ISOLATION OF THE CONTAMINATION

Choosing the technological scheme

Microbiological and agrochemical parameters of treated soil and water

Qualitative and quantitative indicators of oil pollution

The treated object characteristics

I. OBJECTS INVESTIGATION

SCHEME OF WORKS

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STAGES OF WORKS

Engineering investigation and sampling

Strains selection

I. DETAILED ENGINEERING INVESTIGATION1. The site inspection2. Engineering and land inspection3. Geological engineering inspection4. Engineering and environmental inspection (sampling of contaminated

soil and water) II. ANALYSIS

Laboratory microbiological and chemical investigations III. DESIGN WORK1. Drawing 3D map of the contamination

2. Selection of the optimal treatment scheme3. Breeding and selection of useful microorganisms strains-destructors,

effective for this type of pollution4. Choosing remediation strategy 5. Development and coordination of the decontamination project 6. Producing of a biological product

Producing of the bio-preparation


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DRAWING 3D MAP OF THE CONTAMINATION

Stratified 3D map of the pollution General scheme of the contamination


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SOILEX® and fertilizers (N - nitrogen; P- phosphorus; K - potassium) applying

IV. FIELD WORKS

Aeration

Applying of the texture agent (if necessary)

Bio-preparation applying

Watering and fertilizers applying

monitoring

V. HANDING THE OBJECT TO THE CUSTOMER

Control sampling

Independent expertise

plowing (aeration)

Watering

SOILEX® BIOTECHNOLOGYSTAGES OF WORKS

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LowLess than 50 g/kg

Contamination concentration

HighMore than 150 (up to 500) g/kg

SOILEX® and fertilizer (NPK) applying, aeration and watering

Oil recovery

Oil sludge to the special recultivation

site

The residual contamination with the low concentration

Mediumfrom 50 to 150 g/kg

Applying of the texture agent for concentration decreasing

Stock-tank oil

Clean environment

TECHNOLOGICAL SCHEME OF SOIL AND GROUND WATER TREATMENT (depth up to 5 m)

Pumping out the free oil

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LowLess than 30 mg/l

Contamination concentration

HighMore than 80 (up to 500) mg/l

SOILEX® AQUA at the special carrier applying

Oil recovery

Medium concentration

Medium From 30 up to 80 mg/l

Pumping out the free oil (booms, skimmers)

Sorbent applying for concentration decreasing

Stock-tank oil

Clean environment

TECHNOLOGICAL SCHEME OF WATER SURFACES TREATMENT

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EXPERIENCE

Object: "Diamonds of Russia-Sakha"России-СахаMore than 15 000 m3 of oil contaminated soil were treated

More than 22 years at the environmental safety market

More than 500 ha of contaminated soil were cleanedMore than 220 000 m3 of oil sludge were processed

Before the treatment

June

After the treatment

August

The treatment process

July

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Liquidation of the accidental black oil and diesel fuel spill at the rail way station Bologoe (the customer - OAO "Russian Railways")

• The oil film at the water surface was from 1 to 5 mm

• The concentration of oil products in water reached 300 mg/l.

• The length of the trench was 2000 m.

the cleaning was executed by biological method combined with the mechanical one and the sorption technologies

Before the treatment Sorbents applying After the treatmnet

OIL POLLUTED SOIL REMEDIATION

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ACCIDENTAL OIL SPILLS RESPONSEThe train crash at the rail way station Ujta (the customer - - OAO"Russian Railways")

As a result of the executed works the vegetation was almost completely restored, the oil film from water surface was removed.

Before the treatment

After the tratment

• The spill of 80 t of black oil

• About 6 he contaminated

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LIST OF MAJOR EXECUTED PROJECTS OF POLYINFORM IN THE SPHERE OF REMEDIATION PERFORMED DURING 1994 - 2010

31.12.200201.11.1999$6 527 500Cleaning of ground water on the territory of petroleum storage depot (11 hectares)

petroleum storage depot near Tol'jattiД№ 1-11-99

NK «UKOS», «Samaranefteproduct» Corp

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31.10.200601.04.1999$2 348 237Utilization of oil-contaminated soil (2500 m3) on the recultivation site (1800m2)

Locomotive depot of Suojarvirailway station

№ Д04/03-08, №Д05/04-06; №Д06/03-14

JSC "Russian railways", Petrozavodsk branch

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30.12.200501.03.1999$3 082 393Utilization of oil-contaminated soil (5000m3) on the recultivation site (3200 m2)

Locomotive depot at VelikieLuki railway station

№Д03/01-05; №Д04/02-03; №Д04/02-02; Д№05/03-05/ 524/ТЧ-18

JSC "Russian railways", Vitebsk branch

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30.11.200530.12.1998$6 839 673Сleaning and recultivation of soil oil-polluted by the accidental split of 80000 tons of oil

Koptevskij open pit near Ul’janovsk

№Д№1/12-98, №488

JSC "Russian railways", Kujbyshevbranch

3

30.12.200615.10.1998$3 388 934Utilization of oil-contaminated soil (4800 m3) on the recultivation site (3 hectares)

Locomotive depot at Dnorailway station

Д№03/05-01/ 493/ТЧ-18; Д№05/03-07/ 522/ТЧ-18

JSC "Russian railways", Vitebsk branch

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30.10.199415.05.1994$1 378 375Сleaning and recultivation of oil-polluted soil on the site of 8 hectaresUjta railway station№ Д94/05-01JSC "Russian

railways"1

completedstarted

Durationtotal contract amount, USDScope of workObject/LocationContractContractor#

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30.11.200701.10.2003$5 489 737Liquidation of contamination of groundwater on the site of 2,5 hectares and recultivation of polluted soil (accidental split of 2800 tons of oil)

Object “Savvatija”(Archangelskaja oblast)Д№2/003/РMinistry of Defense

of Russia14

30.08.200601.10.2003$3 412 433Liquidation of contamination of groundwater on the site of 3,4 hectares and recultivation of 420 tons of polluted soil

Object “Pribylovo”(Leningradskaja oblast)Д№3/003/РMinistry of Defense

of Russia13

30.11.200301.09.2003$122 449Liquidation of accidental black-oil split on the boiler plant (150 m3 of liquid waste and 430 m3 of soil)

«Heat network», Puskino, Moskow oblast №Д03/05-05

«Heat network», Puskino, Moskowoblast

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30.10.200415.08.2003$1 162 857Cleaning of oil-contaminated sites (3,9 hectares)"Privolzhskie trunk oil-pipelines" Corp (Saratov)

№Д03/07-04/ 200300449 14.08.03

"Transneft" Corp. 11

30.10.200315.07.2003$346 939Cleaning and recultivation of water and soil on the site oil- contaminated because of the train crush (240 tons of oil-contaminated soil)

station-to-station block Station “791 km” –

Shueretskaja№Д03/04-06


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30.09.200301.05.2003$520 255Cleaning and recultivation of oil-contaminated site 2,1 hectares)NK "Sidanko" (Stavropol')№Д03/02-03 "Saratovneftegaz"

Corp. 9

31.10.200601.04.2003$2 373 136Utilization of oil-contaminated soil (2000m3) on the recultivation site (1800 m2)

Locomotive depot of Medvezhja gora railway

station№ Д04/03-06


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10.11.200401.02.2003$2 537 926Liquidation of slime barns and recultivation of contaminated soil (2600 m3)

«Orenburgneft» Corp. (Orenburg)

№03/01-01, №Д03/02-02, №Д03/12-02

«TNK-BP» Corp. 7


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31.10.200601.06.2005$255 680Disposal of black oil-contaminated soil (265 m3) on the recultivation site of locomotive depot

Locomotive depot of Sortavala railway station

№ Д05/04-04, №Д06/03-15

JSC "Russian railways", Petrozavodsk branch 22

31.08.200501.03.2005$842 850Constructing of local treatment plantSortavala railway station№Д04/10-01JSC "Russian railways", Petrozavodsk branch 21

30.11.200412.11.2004$80 612Cleaning of oil-contaminated territory of the plant (180 m3 of soil)

Kirovsky plant (St-Petersburg) № Д04/10-02«Krov-Oil» Inc.20

30.11.200425.08.2004$816 494Recultivation of the oil-contaminated soil on the site of 1,8 hectares

«Saratovneftegaz» Corp. (Saratov)№ 974-ПО«Saratovneftegaz» Corp.19

31.10.200601.04.2004$1 366 437Disposal of oil-contaminated soil (2500 m3) on the recultivation site (1900m2)

Locomotive depot of Petrozavodsk railway

station№ Д04/03-03JSC "Russian railways",

Petrozavodsk branch 18

31.10.200401.03.2004$465 306Utilization of oil-contaminated soil (750 tons) on the recultivation site

Konakovskajahydroelectric plant

(Konakovo)№ Д04/02-01"6 Sigma" Corp.17

30.12.200515.10.2003$1 153 446Utilization of oil-contaminated soil (810 m3) on the recultivation site (2220 m2)

Locomotive depot at Pskov railway station

Д№03/10-02/ 496/ТЧ-18; 1Д№05/03-04/ 525/ТЧ-18,

JSC "Russian railways", Vitebsk branch 16

30.11.200701.10.2003$4 353 457Elimination of contamination (3950 m3) and recultivation of soil on the site of 6,6 hectares

Object "Kozelsk" (Kaluzhskaja oblast)дог. № 1/003/РMinistry of Defense of

Russia15


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15.11.201010.03.2009$ 210 360 780Construction of site for disposal of oil slime with the square of 3.5 ha

“Rosneft-Stavropolneftegaz”Ltd (Neftekumsk)Д09/01/01“Rosneft-Stavropolneftegaz”

Ltd27

In process05.03.2010

Treatment of oil contaminated soil and ground water at the territory of the Refinery with SOILEX biotechnology, the depth of contamination up to 17 m, square - 2 he

«Rosneft-Tuapse Refinery»№ Д09/11-05«Rosneft-Tuapse Refinery»26

15.09.200810.08.2007$1 835 145Cleaning and recultivation of oil-polluted soil (7,8 hectares)

“Gazpromneft-Hantos” Ltd (Hanty-Mansijsk)Д07/07-04 “Gazpromneft-Hantos” Ltd 25

10.10.200727.02.2007$1 306 486Recultivation of the territory of petroleum storage depot (1546 m3 of contaminated soil)

"Aspect" Ltd (Kem')Д07/01-01"Aspect" Ltd24

31.12.200621.07.2005$4 365 913

Cleaning of 2160 tons of oil-contaminated soil from the area of train crash on station-to-station block Zubtsov-Aristovo

recultivation site of Dnorailway station№ Д05/07-03JSC "Russian railways", St-

Petersburg branch 23


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1. Certificate № 159409, «POLYINFORM» service mark, the State register of the trade marks 19.12.97

2. Certificate № 163656, «POLYINFORM» service mark, the State register of the trade marks 30.04.98

3. Federal Service for ecological, technological and nuclear control, License №ОТ-19-0000190 (78) on realization of activities on collecting, using, neutralization, transportation, placing of hazardous waste, 10.03.2009

4. Federal Service for Supervision of the protection of cultural heritage law compliance, License № РОК 00613 on realization of activities on the restoration of the cultural heritage objects (monuments), 17.04.2009

5. The self-regulating organization Non-Commercial Partnership "The Baltic association of designers”, Certificate №0577-2009-78161187545-02 on admission to work on preparation of project documents, which have an impact on the safety of capital construction, 23.12.2009

6. The self-regulating organization Non- Commercial Partnership "The Baltic Constructing Complex”, Certificate №0577-2009-78161187545-02 on admission to the works that have an impact on the safety of capital construction, 30.12.2009

7. NP, «Regional Engineering and Surveying Association», Certificate of admission to the works that have an impact on the safety of capital construction № 0121-FROM-2010-7816118754-01, 26.03.2010

GENERAL:

LIST OF PATENTS, CERTIFICATES AND LICENSES OF POLYINFORM

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1. Certificate № 159410, “SOILEX” trade mark, the State register of the trade marks 19.12.97

2. Certificate № 162746, “SOILEX” Trade mark, the State register of the trade marks 31.03.98

3. The patent for the invention of the Russian agency for patents and trade marks, the method of cleaning soil and water from pollution with oil and oil minerals, the State register of the inventions

4. The patent for the invention of the Russian agency for patents and trade marks, the method of purification of the capillary-porous environment from pollution with oil and oil minerals, the State register of the inventions

5. The patent for the invention of the Russian agency for patents and trade marks, the method of purification the capillary-porous environment from pollution with oil and oil minerals, the State register of the inventions

6. The patent for the invention of the Russian agency for patents and trade marks, the biological product for purification of the environment from oil and oil minerals, the State register of the inventions

7. The patent for the invention of the Russian agency for patents and trade marks, the biological product for purification of the environment from oil and oil minerals, the State register of the inventions

8. The patent for the invention of the Russian agency for patents and trade marks, the bacteria Serratia odorifera A 3пГКМ ВИЗР №99 for oxidation of the Hydrocarbons of oil and oil minerals, Moscow

BIOTECHNOLOGY SOILEX®:

LIST OF PATENTS, CERTIFICATES AND LICENSES OF POLYINFORM

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WE MAKE THE WORLDWE MAKE THE WORLD

ECOLOGICALLY SOUND!ECOLOGICALLY SOUND!

Address: Ligovsky pr., 254, St. Petersburg, Russia, 196084E-mail: [email protected]: http://www.polyinform.com

Tel.: +7(812) 458-85-73 Tel.: +7(812) 458-85-74Fax: +7(812) 458-85-76

Polyinform_recultivation_english

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