KMO Polyclonal ANTIBODY Catalog Number: 10698-1-AP 13 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 10698-1-AP Size: 150UL , Concentration: 260 μg/ml by Bradford method using BSA as the standard; Source: Rabbit Isotype: IgG Immunogen Catalog Number: AG1109 GenBank Accession Number: BC005297 GeneID (NCBI): 8564 Full Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) Calculated MW: 55 aa, 8 kDa Observed MW: 56 kDa Purification Method: Antigen affinity purification Recommended Dilutions: WB 1:500-1:2000 IHC 1:50-1:500 IF 1:10-1:100 Applications Tested Applications: IF, IHC, WB, ELISA Cited Applications: IF, IHC, WB Species Specificity: human, mouse, rat Cited Species: human, mouse, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : mouse heart tissue, HeLa cells, mouse spleen tissue, rat spleen tissue IHC : human kidney tissue, human prostate cancer tissue, human breast cancer tissue, mouse heart tissue IF : HepG2 cells, MCF-7 cells Background Information KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672). Notable Publications Author Pubmed ID Journal Application Ewelina Rojewska 26524415 Neuropharmacology WB Qiongxin Wang 24281189 Circ Res WB M M Guixia Xu 29800553 Brain Res WB,IHC Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.