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Plant Propagation by Cuttings
a. Kinds of cuttings 1) Stem cuttings
2) Leaf-bud cutting (single-eye)3) Root cuttings4) Leaf cuttings
b. Totipotency-The ability of an individual cell to reconstitute the entire plant part and functions
c. Dedifferentiation-The capability of previously developed, differentiated cell to return to a meristem
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Plant Propagation by Cuttings
1. Stem cuttings
2. Leaf-bud cutting (single-eye)
3. Root cuttings
4. Leaf cuttings
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1. Stem Cuttings
• Softwood Cuttings
• Semi-Hardwood Cutting
• Hardwood Cuttings
• Herbaceous Cuttings
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Shoftwood Cuttings - Hydrangea
Butterfly cutting (top)
Double-eye single node cutting (middle)
Single-eye single node cuttings (bottom)
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Softwood Cuttings-Peach
- Actively growing shoots are used
- Softwood cuttings are taken during spring and summer
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Influence of IBA on Semi-Hardwood Cuttings - Cordia
1-Control, 2-50% ethanol, 3-100 ppm, 4-1000 ppm, 5-2000 ppm, 6-4000 ppm, 7-6000 ppm, 8-8000 ppm, 9-10000 ppm IBA
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Semi-Hardwood Cuttings - Jojoba
Treating cuttings in IBA Solution (top)
Sticking IBA-treated cuttings in root substrate (bottom)
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Bottom Heat System for Cutting Benches
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Temperature Differential Helpful for Root Formation
Ambient Temp60 oF
Root zone Temp70 oF
- Rooting requires carbohydrates (energy), auxin (growth hormone), and rooting cofactors (enzymes)
- Temperature differential (10 oF) between the ambient air and root zone is helpful for faster rooting
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Good sanitation is important
Cutting on the left was infected with Alternaria and did not root
Cutting on the right was healthy and rooted well
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Cutting Propagation in Jojoba
• Regular Stem Cuttings– Use 4-5 nodes– Semi-hardwood cuttings
• Single Node Cuttings– Double-eye single node
cuttings– Single-eye single node
cuttings
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Rooting of 3 Different Types of Cuttings in Jojoba
Single Node Cuttings
Double Eye (DE) Single Eye (SE)
5-node
Regular Cuttings
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Single Node Cuttings
Double-Eye Single Node Cutting (DE)
- Healthier than SE
- Less disease attacks
Single-Eye Single Node Cutting (SE)
- Largest no. cuttings/plant
- Slower development
- Higher mortality
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Clonal Selection of Jojoba from Native StandsPima Canyon, Tucson, AZ
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Clonal Selection from Cultivated Field
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Rooting jojoba cuttings in bundles with a bottom heat system
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Jojob Cutting Propagation House
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High Pressure Fogging(1000 psi pressure)
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Cuttings rooted under high-pressure fogging
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Use of Plant Bands for Rooting
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Hardening of rooted cuttings under a shade house
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Clonally Propagated Jojoba FieldBakersfield, CA
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An orchard of clonally propagated citrus plants, Bakersfield, CA
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Commercial Cutting Operation
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Bagged Cuttings are Stored in Cold
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Trimming Cuttings
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Trimming Cuttings
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Growth Regulator (IBA) Treatment on Cuttages
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Rooting Cuttings of Pacific Yew
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Outdoor Rooting Facility-Monrovia Nursery
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Herbaceous Cuttings-Chrysanthemum
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Herbaceous Cuttings
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Carnation Cutting Harvest
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Steam Sterilizing Beds
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pH Adjustment of Carnation Beds
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Planting Rooted Cuttings
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Typical Carnation Benches
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Poinsettia Plant Grown in Mexico
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Commercial Propagation of Poinsettia
Paul Ecke, Sr. – Developer of modern day poinsettias
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Herbaceous Cuttings-Poinsettia
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Leaf Cuttings-African Violet, Piggyback Plant