Pittsburgh PA 15213 Oakland Scientific Conference · 100 Lytton Avenue, Pittsburgh, PA 15213 ... Ohaus Corporation ... Carnegie I Part I: 10:00-10:45 AM Part II: 11:00 – 11:45 AM

Wednesday, May 4, 20169:30 a.m. to 2:00 p.m.

100 Lytton Avenue, Pittsburgh, PA 15213

Formerly the Holiday Inn — same location!Next to Alumni Hall

FROM THE PENNSYLVANIA TURNPIKE • Take I-376 (west) to Exit 7A (Oakland-885N), bearing right

onto Bates Street for one-half mile. • Turn left onto Bouquet Street.• Follow Bouquet Street to Forbes Avenue. • Turn right on Forbes Avenue. • Turn left on S. Bellefield Avenue, continue to Fifth Avenue,

turn left, then right on Tennyson Avenue.

FROM I-79 • Exit I-279 Pittsburgh to I-376 Oakland. • Exit Forbes Avenue.• Turn left on S. Bellefield Avenue and follow directions above.

FROM THE AIRPORT • Follow signs for I-376 East. • Take Exit 3A (Forbes Avenue/Oakland).• Follow Forbes Avenue to South Bellefield Avenue, make a left.• Turn right on Tennyson Avenue.

FISHER SCIENTIFICInvites Laboratory Personnel

to the 22nd Annual

Oakland Scientific Conference

Directions to the Wyndham Pittsburgh University Center

100 Lytton Avenue Pittsburgh PA 15213

Vendor Exhibits

Applied Biosystems

BD

- Biosciences

- Industrial Microbiology

- Life Sciences — Pre-Analytical Systems

Biocision

BioTek Instruments

Boekel Scientific

Brady Americas

Brandtech Scientific

Buchi Corporation

Corning, Inc.

Draeger

Dynalon

Eppendorf North America

Fisher Scientific

- Acros

- Alfa Aesar

- Fisher BioReagents

- Fisher Chemicals

- Fisherbrand

- Isotemp

Ge Healthcare

- Life Sciences Consumables

- Life Sciences Dharmacon

- Life Sciences HyClone

Gilson

Invitrogen

- Consumables

- Evos

Kimberly-Clark

KNF Neuberger

Mettler Toledo

Millipore Sigma

Ohaus Corporation

Sartorius Stedim

Spencer Vernoche/Mott Labs

Spex SamplePrep/CertiPrep

Thermo Scientific

- Auto Liquid Handling

- Cell Culture and Centrifugation

- Chromatography Consumables

- Cold Storage Equipment

- Dionex ICP/GC/HPLC

- FT-IR

- Laboratory Equipment

- Pierce

- Real Time PCR Instruments

- Molecular Biology

- Nalgene/Nunc/MLH/ Orion

- Water Purification Systems

Vistalab Technologies

S E M I N A R S

Morning—Coffee, Tea, Pastries, Bagels in Prehall

Lunch—Served at Noon in Ballroom

Flavored Coffee—Served All Day in Prehall

40+ Vendor Exhibits

Schenley Ballroom

No pre-registration required for vendor exhibits

Technical Seminars

Located in Carnegie I & II – behind Schenley Ballroom

PRE-REGISTER online at:

www.fishersci.com/oaklandconference

Wednesday, May 4, 20169:30 a.m. to 2:00 p.m.

Formerly the Holiday Inn — same location! Next to Alumni Hall

22nd Annual

Oakland Scientific Conference

1. Real-Time PCR Training Seminar

Carnegie I Part I: 10:00-10:45 AM Part II: 11:00 – 11:45 AM

Speaker: Mike Troutman, Senior Scientific Liaison, Thermo Fisher Scientific

The seminar will begin with a basic introduction and tutorial of Real-Time PCR to include terminology, reaction components, amplification, assay design, optimization, reference and control options. Following the introduction, an overview of qPCR-associated applications and solutions will be discussed, to include DNA (mutation detection, single nucleotide polymorphisms/SNPs and high resolution melt/HRM), RNA (gene expression and small RNAs) and protein analysis (protein expression and thermal shift applications).

2. The Science of Western Blotting - Proven Strategies to Optimize Your Western Blots

Carnegie II 10:00 – 10:45 AM

Speaker: Joseph Kramer, Director of Sales, North America, Millipore Sigma

Western blotting is a cornerstone of protein research and is used extensively to detect and quantify proteins in complex mixtures. Optimization of reagent concentrations, materials and technique is key to successful blotting. Rethink Western blotting to take protein detection to new dimensions. The SNAP i.d. 2.0 unique vacuum-driven technology promotes antigen binding and thorough washing, enabling you to better optimize your Western blotting conditions - in 30 minutes or less.

3. The Current Landscape of Biological Testing using High Content Analysis

Carnegie II 11:00-11:45 AM 12:45 – 1:30 PM

Speaker: Nicholas M. Radio, Ph.D., Technical Sales Specialist, Thermo Fisher Scientific

High Content Analysis is an image cytometry-based methodology used to measure cellular and small organism biology. The automated nature of the imaging and quantitative analysis enables the ability to measure numerous timepoints and concentrations that are conventionally difficult to accomplish using manual methods. This technical seminar will provide an overview of applications where the technology is applied, including receptor activation, cellular signaling, viability, morphology and motility phenotypes.

4. Materials and Systems for Optimizing 3D Cell Culture Environments

Carnegie I 12:45-1:30 PM

Speaker: Mark Rothenberg, Manager Scientific Training and Education, Corning, Inc.

Three-dimensional (3D) cell culture environments provide structural and biochemical cues for cellular differentiation and functionality. For specialized cell types such as primary cells and stem cells, a two-dimensional (2D) growth sub strate may not be sufficient to support complex cellular behaviors such as cell polarity, morphology, spheroid forma tion, signal transduction and tissue-specific gene expression. This seminar will highlight applications that rely on the use of 3D growth substrates and specialized culture systems (i.e., permeable supports).The biological and functional properties of a variety of cell types will be discussed to demonstrate the effectiveness of these environments.

Seminar pre-registration suggested: www.fishersci.com/oaklandconferenceContinental Breakfast at 9:30 A.M. — Lunch at Noon

No charge for Seminars

40+ Supplier Exhibits

Please stop by!!

Questions? Email:

Janice Brophy — [email protected]

or

Mary Ann Prignon — [email protected]