Piperine Pilot Ppt

PILOT SCALE TECHNIQUE FORpiprine FROM black pepper

BYK.SUDHAKAR

I M.PHARMACY

• PLANT: It is considered as the place where material,men,method and machine are brought together for the manufacturing of products.

• PILOT SCALEUP: It refers to the art of designing a lab scale formula for the production.

PILOT SCALE TECHNIUE

Definition:

A pilot plant allows investigation of a product and process on an intermediate scale before large amounts of money are committed to full-scale production.

WHY TO CONDUCT PILOT PLANT STUDIES

• A pilot scale up technique is an intermediate scale for estimation & assessment of a product and process on before huge amounts of money invest for large scale production.

• It is not possible to design a large scale processing plant from laboratory data alone with any degree of success.

PIPERINE

PIPERINE PROCESSING Collection & Cultivation. Extraction. Analysis. PACKING & STORAGE.

Piperine

• Piperine is extracted from the plant Black pepper i.e Piper nigrum linn., of the Piperaceae family.

Geographical Distribution : The plant is widely cultivated in India, Ceylon and other tropical countries. This climbing perennial shrub grows in hot and moist places.

Classification:

• Kingdom : Plantae• Division : Angiosperma• Class : Dicotyledonae• Order : Piperales• Family : Piperaceae• Genus : Piper• Species : nigrum Linn.

Vernacular names:

• Sanskrit : Maricha, Ushana• Hindi : Kali mirch• Kannada : Karemenasu• Marathi : Kali mirch• Gujarati : Kalamari• English : Black pepper• Bengali : Kalamorichi• Telugu : Miriyala

• (c) Part used : Fruits

MACROSCOPY

• The entire fruit almost globular about 4- 6.5mm diameter, brownish to black in colour, the surface is uneven , seeds almost white, aromatic with a pungent taste

CULTIVATION & COLLECTION

Cultivated area & Yield in India: Cultivated States: Climate, Rain fall and Altitude: Soil : Fertilizers & Manures: cuitivation is done by s Sowing owing seeds

Max yield:100 kg

CHEMICAL CONSTITUENTS IN PEPPER

Piperine 5-9%Volatile oil 1-2.5%Piperidine and resin 30% The volatile oil is yellow in colour contains mainly l-phellandrene and caryophyllene.

Chemial structure:

Mol. For C17H19NO3 Mol..Wt. 285.34

USES OF PepperThe health benefits is

Digestive, Carminative, Stomachic Aromatic Se bio availability of certain drugs.

TYPES OF DRYING

Dryness (%) = Initial weight of sample (g) - Current weight of sample Dryness (%) (g)|x 100 Initial weight (g)

EXTRACTION

Methods Used For Extraction Of Oil:

•Infusion•Maceration•Percolation•Soxhlation•Soxtec•M.A. E•Ultra Sonication•Spouted Bed ExtractionSteam Distillation:Super Critical Fluid Extraction (SFE):•R.P.E•MPSLE

The pilot scale water distillation unit

Condenser

Dean stark

Separating funnel

Pressure cooker

The water and steam distillation unit at CLEAR

SUPERCRITICAL FLUID EXTRACTION (SFE)

TLC PROFILE OF PIPERINE

Sample detail : Piperine( 98%)

Adsorbent : Precoated silicagel 60

Mobile Phase : Benzene: Ethyl Acetate (2:1)

Sample preparation : 1o mg/ml of piperine was

dissolved in methanol and 10 µl applied

on the TLC plate.

Solvent front run upto : 80 mm

Detection : By Vanillin sulphuric acid (Fig.2).

Scanning : 254 nm (Fig.1).

Fig. 1

Fig. 2

~

S T

FTIR In KBr Dispersion

PACKING AND STORAGE

ppiperine

References:

1. Pharmacognosy, by Dr.C.K. Kokate , A.P. Purohit,S.B. Gokhale Nirali

Prakashan, 38 th edition, 2007.

2. Qulity control of herbal drugs by Pollok Mukhergy

3. Wagner H, et al, Plant Drug Analysis, Spinger, 1996, 300

4.British Pharmacopoeia, 305

5. Indian Standard Specification for Oil of Ginger, 1st Revision.

6. Herbal Drug Technology by Prof S.S Agarwal 7. Online library wiley.com