DEMONSTRATION OF LARGE DEMONSTRATION OF LARGE SCALE PRODUCTION OF OLIGO SCALE PRODUCTION OF OLIGO CHITOSAN CHITOSAN GATOT TRIMULYADI REKSO GATOT TRIMULYADI REKSO E-mail: [email protected]E-mail: [email protected]CENTER FOR THE APPLICATION OF ISOTOPE CENTER FOR THE APPLICATION OF ISOTOPE AND RADIATION TECHNOLOGY AND RADIATION TECHNOLOGY NATIONAL NUCLEAR ENERGY AGENCY NATIONAL NUCLEAR ENERGY AGENCY (Augustus 4th – 8 th - 15th, 2008) 1 Dr.Ir.Gatot Trimulyadi Rekso- Dr.Ir.Gatot Trimulyadi Rekso- email:[email protected]email:[email protected]
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DEMONSTRATION OF LARGE DEMONSTRATION OF LARGE SCALE PRODUCTION OF OLIGO SCALE PRODUCTION OF OLIGO
DEMONSTRATION FOR 1000 L OLIGO-DEMONSTRATION FOR 1000 L OLIGO-CHITOSAN- LIQUID IRRADATION CHITOSAN- LIQUID IRRADATION
PROCESSPROCESS Raw materialRaw material - Chitosan 20 kg - Silver Nitrate 500 g- Chitosan 20 kg - Silver Nitrate 500 g - Lactic acid 20 L - NaOH pa 1 Kg- Lactic acid 20 L - NaOH pa 1 Kg - Demineral water 1000 L- Demineral water 1000 L MachineMachine - Mixing tank 1200 L - Raw mat storage- Mixing tank 1200 L - Raw mat storage - Irradiation tank 1500 L - Product storage- Irradiation tank 1500 L - Product storage - Liquid transfer system- Liquid transfer system Irradiator Irradiator ƴƴ – Co-60 – Co-60
Step for demonstration of Step for demonstration of preparing oligo-chitosanpreparing oligo-chitosan
Standardization of chitosanStandardization of chitosan Mixing processMixing process Transfer process to irradiation tankTransfer process to irradiation tank Irradiation ProcessIrradiation Process Finishing processFinishing process Preservation ProcessPreservation Process PackingPacking
MIXING PROCESS ( 500 L)MIXING PROCESS ( 500 L)- Put a DM water 100 L to mixer tank - Add chitosan 10 Kg - Add DM water 100L - Keep for 1 hr - Add lactic acid 10 L - Stirr for 1 hr - Adding DM water up to 500 L (around 285 L)
Preparation of Ag-nano/chitosan for utilization as preservative 1. Taking 10 litters of chitosan solution as above mentioned preparation 2. Adding 17 g AgNO3 (disolved in small volume of water) into chitosan
solution 3. Stirring about 15 minutes 4. Putting prepared Ag+/chitosan solution into plastic can 5. Irradiation at dose of 30 kGy 6. Ag-nano/chitosan colloid with concentration of 1.000ppm Ag.
Preservation of oligo-chitosan with Preservation of oligo-chitosan with Ag-Nano of 20 ppmAg-Nano of 20 ppm
Taking 25 L oligo-chitosan that already Taking 25 L oligo-chitosan that already neutralization by 2M NaOHneutralization by 2M NaOH
Adding 500 mL Ag-nano/chitosan little Adding 500 mL Ag-nano/chitosan little by little while stirringby little while stirring
Stir for 30 minStir for 30 min Filter with net mesh (# 100)Filter with net mesh (# 100) Move to plastic drum of 30 L for Move to plastic drum of 30 L for
1. For Rice : Dilution 1000 times with water (20ppm), spraying 3 times (20,30 and 40 days after sawing), 500 L diluted/ha it means 1.5 L (2% of original oligo chitosan)/ha Seedling : For rice seed treatment, dilution 100 times with water (1/100). Rice seed are dipped in oligo chitosan solution about 10 min
2. For other plants such as tea, potato, carbage, sugarcane, carrot, red peper, tomato and any kind flower : dilution 500 times with water (40 ppm) spraying 3 time up to haverst