Pharmaceutical analysis, tech & implementation

Md. Obydulla Al Mamun

Id:131-29-500

Department of pharmacy

Daffodil international university

Pharmaceutical analysis, tech & implementation

Analytical techniques

1. Titrimetric techniques

2. Chromatographic

2.1. Thin layer

chromatography

2.2. High performance thin

layer chromatography.

2.3. High-performance liquid

chromatography (HPLC)

2.4. Gas chromatography

3. Spectroscopic techniques

3.1. Spectrophotometry

3.2. Near infrared spectroscopy (NIRS).

3.3. Nuclear magnetic resonance spectroscopy (NMRS)

3.4. Fluorimetry and phosphorimetry .

4. Electrochemical methods

5. Kinetic method of analysis

6. Electrophoretic methods

7. Flow injection and sequential injection analysis

8. Hyphenated techniques

Titrimetric Analysis

• Measurement of the volume of a unknown solution (the analyte) with a known

concentration of a reagent(the titrant) .

• Titrimetric methods are widely used for routine analysis because they are rapid,

convenient, accurate, and readily automated.

Spectroscopic techniques

• The use of spectroscopy in determining the

chemical or physical constitution of substances.

• Classification:-

1.Spectrophotometry

2.Near infrared spectroscopy (NIRS).

3.Nuclear magnetic resonance (NMR)

4.Fluorimetry and phosphorimetry .

1. Spectrophotometry 2.Near infrared spectroscopy

• Quantitative measurement of

the reflection or transmission

properties of a material as a

function of wavelength.

• The advantages of these

methods are low time and

labor consumption.

• Provides multi component

analysis of almost any matrix.

• Used in pharmaceutical

industry for raw material

testing, product quality control

and process monitoring.

4.Fluorimetry and phosphorimetry3.Nuclear magnetic resonance

spectroscopy (NMR)

• For serve the purpose of

high sensitivity without

the loss of specificity or

precision.

• Used To determination of

protein content of

commercial foods.

• An analytical technique

used in quality

control and research for

determining the content

and purity of a sample

as well as its molecular

structure.

Electrochemical methods

• The study of an analyte by measuring the potential (volts)

and/or current (amperes) in an electrochemical cell containing the

analyte .

Chromatography

•A method of separating and analyzing mixtures of chemicals.

•The separation by allowing a solution or mixture to seep through an adsorbent

(such as clay, gel, or paper) so each compound becomes adsorbed into a separate.

Use of Chromatography

HPLC• HPLC-high performance liquid chromatography

• Also called high pressure liquid chromatography

• HPLC has been around for about 35 years and is the largest separations technique used

• HPLC is really the automation of traditional liquid chromatography under conditions which provide for enhanced separations during shorter periods of time, utilizing very small particles, small column diameters, and very high fluid pressures.

How HPLC work?

Advantages

• It can be applied to the separation and analysis of

complex mixtures.

• Accurate quantitative measurements.

• Needs a small sample with a high accuracy

•Need a skill to run the instruments

•Solvents consuming

Disadvantages

Application of HPLC

1. Pharmaceuticals industry

• To control the drug stability

• Quantity of drug determination from pharmaceutical dosage forms, ex. Paracetamol determination in panadol tablet

2. Analysis of natural contamination

- Phenol & Mercury from sea water

4. Food manufacture

- sweetener analysis in the fruit juice

- preservative analysis in sausage etc.