M13 coat protein 3 (g3p), present on average in 5 copies at the tip of the M13 phage particle, is often the first choice for phage display fusions because of its tolerance for large insertions. Filamentous phage M13 is the mostly commonly used for antibody display. Directly insertion makes the phage plasmid too large to transfection and amplification. A phagemid and helper phage system is commonly used for antibody display. Monovalent display is preferred due to the demanding of high affinity monoclonal Abs. g1p: Morphogenesis g4p, M13 T7 T4 λ G7P G9P G8P ssDNA M13 6.4Kb G9P G3P g2p: RF replication Endonuclease g10p g5p: ssDNA binding g7p: Minor capsid g8p: Major capsid g3p: Absorbtion protein g6p: Minor capsid Phage: structure and genome Phagemid system for antibody display pHAL30 Ab Heavy Chain bla g3p Lac Pr. Stuffer fragment Ab Light Chain Phagemid E. coli Helper Phage Packaged Phage with antibody displayed on surface Antibody G3P Ab DNA library Drug Discovery Phage plasmid Recombinant phagemid 2-3 Rounds High affinity mAb Weak affinity Abs Affinity screening Binding and Wash Phage display library Eluting Amplify Diagnostics Antibody Production 1 2 Phage display library and screening 3 Solid surface immobilized with antigen is used for antibody phage screening. Antibody phage particles that bind weakly to the antigen and the vast excess of non-binding antibody phages are removed by stringent washing. Specifi- cally binding antibody phage are eluted and used for infection of for the next round screening. 2-3 round of screening are necessary for monoclonal antibody with high enough affinity. Immune, naïve or synthetic library construction Pre-made library screening Large library capacity High affinity from 10 -7 to 10 -9 Various phage display systems (M13, T4, T7) Tailored biopanning strategies Phage display library construc- tion Phage display library screening Specific antibody discovery Peptidomic discovery FEATURES: PHAGE DISPLAY TECHNOLOGY Creative Biolabs Phage Display & Antibody Library Services WHAT WE DO: © 2007 - 2018 Creative-Biolabs All Rights Reserved Email: [email protected] Tel: 1-631-381-2994 E. coli E. coli.