Ph meter

1pH meter

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2pH Meter

3ContentIntroductionHistory PrincipleConstruction of a pH meterTypes of pH meterTypes of probe / electrode.CalibrationMeasurementApplications ConclusionReference

4IntroductionA pH meter is an electronic instrument used for measuring the pH (acidity or alkalinity) of a liquid (though special probes are sometimes used to measure the pH of semi-solid substances). A typical pH meter consists of special measuring probes (a glass electrode and a reference electrode) connected to an electronic meter that measures and displays the pH reading.

Source : http://en.wikipedia.org/wiki/PH_meter

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The first commercial pH meter was built around 1936 by Radiometer in Denmark and by Arnold Orville Beckman in the United States.

HistorySource :http://en.wikipedia.org/wiki/PH_meter

6PrinciplepH is defined as the negative logarithm of hydrogen ion concentration.pH= -log [ H+ ]p = powerH = hydrogen[H+ ] = hydrogen ion concentrationThe pH of a solution can be measured by the pH meter. The glass electrode is an half cell and the calomel electrode is another half cell.

7Following reactions take place in the electrodes

The glass electrode contains Ag, AgCl, and HCL. All these remain in the ionized state.Ag Ag+ + eAgCl Ag+ + Cl-HCL H+ + ClAll the above three equilibrium reactions are balanced. In the glass electrode, H+ is generated.The calomel electrode contains Hg, Hg2Cl2 , and KCL.Here also the following series of ionizations take place.Hg Hg+ + e-Hg2Cl2 2Hg+ + 2Cl-KCL K+ + Cl- The above equilibrium reactions are balanced. In the calomel electrode, H+ is not generated.

8The pH scaleFigure : pH scaleSource :http://floridacoastalcooking.blogspot.in/2012/03/how-important-is-ph-in-body.html

9Table showing different pH values

Litmus dye is a natural pH indicator . Litmus paper is a small strip of paper that has dyes in it. When it touches a base or a dye then the litmus paper changes colors depending on , either it is a base or it is an acid. If a litmus paper is exposed to an acid it either changes its colour to pink or red. If a strip of litmus paper touches a base then the paper changes to a shade of blue.

10pH strips

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Figure : pH values of different samplesFigure : litmus test

12Construction of pH meter

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Figure : manual pH meterTypes of pH meterManual pH meterDigital pH meterSource : http://doubleroptics.tradeindia.com/deluxe-ph-meter-226808.htmlA manual pH meter

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Figure : Digital pH meterSource : http://en.wikipedia.org/wiki/PH_meterA digital pH meter

15Types of probe 1. Glass electrode2. Reference electrode 3. Combination gel electrode

Figure :Glass electrodeFigure : Reference electrode

Source :http://www.alliancets.com/site/files/408/29497/109873/159912/Guide_to_pH_Measurement.pdf

16Figure : Combination gel electrode

Source : [http://www.ph-meter.info/pH-electrode-flowing-gel

17CalibrationCalibration should be performed with at least two standard buffer solutions that span the range of pH values to be measured.For general purposes buffers at pH 4 and pH 10 are acceptable.The pH meter has one control (calibrate) to set the meter reading equal to the value of the first standard buffer and a second control (slope) which is used to adjust the meter reading to the value of the second buffer.A third control allows the temperature to be set.Source :http://en.wikipedia.org/wiki/PH_meter

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20ConclusionA pH meter is an electronic instrument used for measuring the pH of a liquid/semi-solid substances. We exploited the properties of this indicator to determine with precision the acidity or alkalinity of various substances. pH meter is more useful than the other pH indicators because it gives accurate reading (e.g. , for detection of blood pH ) and can measure the ph of a liquid as well as a semi-solid substance.

21Reference Wilson Keith & Walker John, (2008), pH and oxygen electrodes, Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press, 6th edition. Pg. 18-23Sourcehttp://en.wikipedia.org/wiki/PH_meterhttp://www.alliancets.com/site/files/408/29497/109873/159912/Guide_to_pH_Measurement.pdf