PDGF-B Is Required for Development of the Glymphatic System€¦ · of the Glymphatic System Anne Sofie Munk, Wei Wang, Nicholas Burdon Bèchet, Ahmed M. Eltanahy, Anne Xiaoan Cheng,

Cell Reports, Volume 26

Supplemental Information

PDGF-B Is Required for Development

of the Glymphatic System

Anne Sofie Munk, Wei Wang, Nicholas Burdon Bèchet, Ahmed M. Eltanahy, Anne XiaoanCheng, Björn Sigurdsson, Abdellatif Benraiss, Maarja A. Mäe, Benjamin TravisKress, Douglas H. Kelley, Christer Betsholtz, Kjeld Møllgård, Anja Meissner, MaikenNedergaard, and Iben Lundgaard

FigureS1.VentralviewoftracerinfluxinP1,P7andP14mice,RelatedtoFigure1

DorsalviewofwholebrainsshowingdistributionofCSFtracersFITC-dextran(dextran-3kDa)

and ovalbumin-Alexa647 (OA-45 kDa) 60 min after injection into the cisterna magna. P1

mousebrain(toppanel)showsdistributionoftracersaroundtheCircleofWillisandalongthe

proximalpartofthemiddlecerebralartery(MCA). Imagesofdextran-3kDaandOA-45kDa

tracersinP7(middlepanel)andP14brain(bottompanel)showtracerdistributionaroundthe

CircleofWillisandtheMCAs.

FigureS2.WesternblotsofAQP4vessel–andparenchymalfractionsduringdevelopment,

RelatedtoFigure3

A-D) RepresentativeWestern blots of parenchyma fractions and isolated vessel fractions

incubatedwithprimaryantibodiesagainstaquaporin4(AQP4,38kDa);andb-tubulin(52kDa).

A)Theblotwascoveredstartingfromthedottedlineandupwardstoavoidstrongsignalfrom

off-targetbands.CTX,cortex;HPC,hippocampus;P,postnatalday;M,proteinmarker;CTR

Ag.,AQP4controlantigen.

FigureS3.WesternblotsofAQP4vessel–andparenchymalfractionsfromPdgfbret/ret

mice,RelatedtoFigure6

A-B) Representative Western blots of parenchyma fractions and isolated vessel fractions

incubatedwithprimaryantibodiesagainstaquaporin4(AQP4,38kDa);andb-tubulin(52kDa).

A)Theblotwascoveredstartingfromthedottedlineandupwardstoavoidstrongsignalfrom

off-targetbands.CTX,cortex;HPC,hippocampus;M,proteinmarker;CTRAg.,AQP4control

antigen;WT,wildtype;R/R,Pdgfbret/ret;R/+,Pdgfbret/wt.