IPICEX 2008 1 Mohsin, H. F., Abdul Wahab, I., Naz, H., Ali Shah, S. A. and Weber, J. F. F. Faculty of Pharmacy, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan Pandanaceae Research in Support for Industrial Plantation 2 nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18 th - 21 st November 2008 OBJECTIVE To present the chromatographic profile of the root extract of Pandanus sanderi. Alkaloids, terpenoids and phytosterols are Pandanus’ major constituents. HYPOTHESIS 2 nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18 th - 21 st November 2008 5 2 9 8 7 4 1 HO 3 6 10 11 12 13 N O O CH 3 O H H O H 3 C 16 15 4 5 H 18 20 21 2 N O O CH 3 O H H O H 3 C 16 15 4 5 H 18 20 21 2 9 8 9 8 7 N O O CH 3 O H H O H 3 C 16 15 4 5 H 18 20 21 2 9 8 7 O
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Pandanaceae Research in Support for Industrial … 2C Mohsin...IPICEX 2008 4 • Pandanus fruits were reported to contain provitamin A carotenoids (Englberger, L. et al., 2003; 2006).FOOD
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IPICEX 2008
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Mohsin, H. F., Abdul Wahab, I., Naz, H., Ali Shah, S. A. and Weber, J. F. F.
Faculty of Pharmacy, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan
Pandanaceae Research in Support for Industrial Plantation
Pandanaceae Research in Support for Industrial Plantation
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
OBJECTIVE
To present the chromatographic profile of the root extract of Pandanus sanderi.
Alkaloids, terpenoids and phytosterols are Pandanus’ major constituents.
HYPOTHESIS
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
5
2 9 8
74
1
HO3
6
10
1112
13
NO
O
CH3
O
HH
OH3C
1615
4
5
H
1820
21
2N O
O
CH3
O
HH
OH3C
1615
45H
1820
21
2
9
8
9
8 7
N
OO
CH3
O
HH
OH3C
1615
4
5
H
1820
21
29
8 7
O
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EXAMPLES OF PANDANACEAE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
•Unlike the green Pandanus amaryllifolius (pandan wangi), P. sanderi is variegated or having streaks of a different colors. •It is characterized by longitudinal yellow and green stripes of the leaves. •The yellow stripe is at the middle of two green stripes.
• In India, the leaf extract is used to cure thyroid problem and to treat fever in Taiwan (Jong, T.Jong, T.--T. et T. et al., 1998al., 1998).
• Several species were reported to act as a remedy for toothache and rheumatism (Takayama, 2000)
• Busque, (2002) reported that P. amaryllifolius was used for strengthening the heart and as a diuretic.
PHARMACEUTICAL PERSPECTIVE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
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Various compounds from Pandanaceae species.
Isolated compounds from Pandanaceae species
References
1 Polypeptide Ooi, L. S. M. et al. (2004)2 Alkaloids Salim, A. A. et al (2004)
3 Pyrrolidine Alkaloids Takayama, H. et al. (2002)
4 Alkaloids Takayama, H. et al. (2001)
5 2 phenolics, four lignans Jong, T.-T. et al (1998)
6 4-Hydroxybenzoic acid Peungvicha, P. et al (1998)7 Isopentenyl and dimethylallyl
acetates and cinnamatesI. Vahirua-Lechat et al (1996)
8 Piperidine alkaloids Nonato, M. G. et al. (1993)
9 Sesquiterpene hydrocarbons and monoterpene (linalool)
Macleod, A. J. et al. (1982)
PHYTOCHEMICAL PERSPECTIVE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
NO
O CH3
OO
CH3
N
OOCH3
OH3C
O
H
Pandamarilactone-1C18H25NO4
PandanamineC18H23NO4
NO
O
CH3
Pandamarilactonine-AC18H25NO4
O
HH
OH3C
O
OH3C
O
O
CH3
An compound which is not a "natural product" (Salim et al., 2004)
C18H23NO4
16
16
N
NH
O
HH
OH3C
norpandamarilactonine-A norpandamarilactonine-B
NH
O
HH
OH3C
PHYTOCHEMICAL PERSPECTIVE2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
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• Pandanus fruits were reported to contain provitamin A carotenoids (Englberger, L. et al., 2003; 2006).
FOOD & BIOTECH PERSPECTIVE
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
METHODOLOGY
• Liquid-liquid partition
• Column Chromatography
• Thin layer chromatography
(TLC)
• Preparative TLC
• HPLC
• Spectroscopy Analysis (NMR)
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
• Sample extraction
• Fractionation of crude extract
• Isolation of pure compounds
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Outline of the normal extraction procedure
Hexane extractChloroform extractMethanol extract
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
Grinding Soaking
RESULTS & DISCUSSION
TLC of the root extract: short wave (hexane:acetone = 80:20)
TLC of the root extract: long wave (hexane:acetone = 50:50)
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
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Results and discussion : Chromatograms
(i) λ=210, 280 and 360 nm
(ii) λ=210 nm (iii) λ=280 nm (iv) λ=360 nm
C18 column: (i) 4.6 x 150 mm
C18 column: (ii) – (iv) 4.6 x 250 mm
Pandanus sanderi- Root- MeOH fraction
HPLC Agilent Technologies' Zorbax® C-18; 4.6 x 250 mm, 5 μm, CH3CN:H2O, 10 to 90%; 38 min, flow rate: 1 ml/min, λ=210, 280, 360 nm
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Pandanus sanderi- Root- Hexane fraction
HPLC Agilent Technologies' Zorbax® RX-Silica; 4.6 x 250 mm, 5 μm, Hexane:EtOAc, 10 to 90%; 40 min, flow rate: 1 ml/min, λ=210, 280, 360 nm
Results and discussion : Chromatogram
The chromatograms that were produced, with and without the utilization of the analytical guard column (4.6 x 12.5 mm).
•tR = 18 – 36 minutes could be isolated and subjected for fractionation.
•The collection methods (at λ=210 nm) are currently in progress, with the employment of the analytical guard column.
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CONCLUSION
Pandanaceae will gain more attention in pharmaceutical investigations, food research and urban landscape improvement.
Future planning for a Pandanaceae plantation could encourage research and development in industrial, environmental and eco-tourism sectors.
2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
Beentje, H. 1993. Pandanaceae: Ed. R. M. Polhill. Kew Royal Botanic Gardens, Royal Botanic Gardens, Kew, R. M. Polhill, East African Herbarium, East African Herbarium, CRC Press.
Chih Min, B., Omar-Hor, K. & Chow Lin, O.-Y. 2003. 1001 Garden Plants in Singapore. National Parks, Singapore. pp 184.
Tan, M. A., Takayama, H., Aimi, N., Kitajima, M., Franzblau, S. G. & Nonato, M. G. 2008. Antitubercular triterpenes and phytosterols fromPandanus tectorius Soland. var. laevis. J Nat Med, 62, 232–235.
Mohsin, H. F., Abdul Wahab, I., Naz, H., Ali Shah, S. A. & Weber, J. F. F. Chemical Constituents Of Pandanus sanderi (Pandanaceae). National Conference on Forest Products 2008 (NCFP 2008), 28th – 31st October 2008, Kuala Lumpur.
REFERENCES2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008
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• Mr. Mohamad Soliheen Bin Mohd. Zaki & Mr. Mohd Rizal Bin Salleh for their technical assistance, under the university’s Student Employment Scheme.
• Ms. Suriana Hanim bt. Ariffin @ Sukri for the literature search.
• Faculty of Pharmacy UiTM, for the support.
Acknowledgements 2nd International Plantation Industry Conference and Exhibition (IPiCEX 2008), Shah Alam, 18th - 21st November 2008