PacBio - Galaxy · unmethylated (bottom) adenosine. (b) Typical SMRT sequencing fluorescence traces from these templates. ... NGS101-PacBio.key Created Date: 3/4/2015 4:37:49 PM ...

PacBio

2003

Levene et al. 2003

Korlach et al. 2008

Eid et al. 2009

Flushberg et al. 2010

Stats

‣ Mean RL ~ 3,000 bp ‣ 95th percentile ~ 8,000 bp ‣ Max RL ~15,000 bp ‣ 30,000 - 50,000 reads ‣ 100 - 150 Mb/ SMRT cell ‣ Runtime 90 min

Types of runs

‣ CLR: Continuous Long Reads ‣ CCS: Circular Consensus Sequencing

Error profile and GC bias

Carneiro et al. 2012

Carneiro et al. 2012

Loading regimes

‣ Diffusion loading ‣ Magnetic bead loading

Modification detection

Flusberg et al. 2010

Error correction

Koren et al. 2012

Error correction

Koren et al. 2012

Closing gaps

Chaisson et al. 2015

Closing gaps

Chaisson et al. 2015

Transcript sequencing

Tilgner et al. 2014