P18082 ELECTRICAL BIOREACTOR (EBR) Emily Kimber, Jonathan Girard, Shena Marshall, Dillon Flood, Luc Belikis INTRODUCTION DISCUSSION & CONCLUSION RESULTS Acknowledgements Selected carbon electrode material based on media pH tes@ng Defined sta@c voltage range (0V2V) & recommended applied voltage range (0.2V 0.5V) Assembled durable and ergonomic bioreactor: o holds cell culture (cells and media) o applies voltage to cell culture o visible Arduino o intui@ve LabVIEW design o low assembly and disassembly @me Maintained culture condi@ons of 5% CO2 and 37°C in incubator Sustained media pH levels in acceptable 7.0 7.6 pH range Observed 3T3 cell growth and development over a week period Michael Zona, Jennifer Bailey, Vinay Abhyankar, Steven Day, Michelle Horan, Robo@cs Laboratory, RIT Machine Shop Figure 5. Custom Carbon Electrodes. Cells are the basic building blocks of all living things: providing structure, nutrients, and specialized func@ons. Cell culture is the act of growing cells under specific environments outside the body to determine if internal and external factors affect cell development. An electrical bioreactor has been designed and manufactured to assist the Advanced Cell Culture Techniques course offered at Rochester Ins@tute of Technology for undergraduate biomedical students to study the process cells undergo during cell culturing and the development process. Figure 6. Isometric (top) and Back View (bosom) Electric Bioreactor Final Design. METHODS Based on an 83% project success rate with regards to engineering requirements, future work includes: further developing code to include change in frequencies and voltage cycles with the addi@on of cyclic. determining personal preference in cell type for electrical s@mula@on and applied voltage values. Figure 3. Isometric View of Concept Design 1 (leB) and 2 (right). Figure 2. Systems Architecture. Figure 8. Customer Requirements. Figure 4. Electrode Material Tes@ng Results. Figure 7. LabVIEW User Interface (top) and code (bosom). Figure 1. Morphological Chart. Dr. Jennifer Bailey, Senior Lecturer ([email protected]) Website: hsp://edge.rit.edu/edge/P18082/public/Home Figure 9. Engineering Requirements.