P12-45

CONTACT :

Dr. D Inbakandan, Scientist –C

Centre for Ocean Research Sathyabama University Chennai - 600 119, Tamil Nadu, India. Email : [email protected] Ph:91 44 - 24500646 Fax 9144-24500646

Mobile : 91-9965540310Mobile : 91-9965540310

Titanium Silanased SNP coated

SEM IMAGE

Conclusion :

Marine biolm formation on surfaces exposed to seawater in vitro for 10 days. After retrieval from seawater the surfaces were scanned with electron microscope acridine orange staining and live/dead Baclight uorescence staining. Low marine biolm formation and hydrophobic nature qualies the modied surface for marine antifouling applications

Characterisation of Silver Nano Particles

A band observed in UV Visible spectrum corresponding to the Surface Plasmon Resonance occurs at 424 nm

Histogram revealed almost 20% of the particles were 24 nm in diameter

HRTEM graphs revealed the different sizes of the silver nanoparticles, ranging from 15 nm to 34 nm in diameter

The FTIR spectrum of silver nanoparticles biosynthesized by sponge extract of A. elongata shows peaks at 3345, 2078, 1634, 1031 and 627 cm−1

XRD analysis are assigned to the crystal structure of the face-center-cubic (fcc) silver.

FTIR spectrum of the sponge extract of A. elongata shows peaks at 3255, 2039, 1628, 1006 and 662 cm−1

Nano Silver Assembled Conventional Metal Surface for

Marine Antifouling ApplicationsD. Inbakandan1,4, V. Sivakumar2, Dune Naga Ravindra2, C. Kumar1, R. Venkatesan3, S. Ajmal Khan4

1. Centre for Ocean Research, Sathyabama University, Chennai 600 119, India2. Department of Biotechnology, Sathyabama University, Chennai 600 119, India3. National Institute of Ocean Technology, Pallikaranai, Chennai-600036, India4. Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai 608 502, India

Abstract

AA simple wet-chemical technique for the assembly of silver nanopartilces on conventional metal substrates, which alleviates the need for lithography, has been developed. Silver nanoparticles were prepared by the biological reductionreduction of silver precursors and attached to silane functionalized Stainless steel surfaces to create a Self Assemble Monolayer with anti-microfouling properties. The assemblies of silver nanoparticles on surfaces were characterized by SEMSEM and Energy Dispersive X-ray Spectroscopy.The silver nanoparticles diameter was estimated by TEM analysis. Detection and measurement of the energy permits elemental analysis of surface revealed the coatingcoating nature and silver nanoparticles deposition. Contact angle measurements revealed the hydrophobic nature and surface energy of the prepared surfaces. This work reported the marine biolm formation on surfaces exposedexposed to seawater in vitro for 10 days. After retrieval from seawater the surfaces were scanned with electron microscope and the images are compared for marine biolm formation. Biolm bacterial attachments on the surfacessurfaces were studied by acridine orange staining and live/dead Baclight uorescence staining. Low marine biolm formation and hydrophobic nature qualies the modied surface for marine

antifouling applications.

A color change to yellowish brown conrmed the biosynthesis of silver nanoparticles.

10 ml of aqueous sponge extract was added to 100 ml of 10−3 M AgNO3 aqueous solution and kept at 45°C for 4 hours in continuous stirring.

Biosynthesis of Silver Nanoparticles

Species : Acenthella elongata