Oxi proteomics

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Aging, UV Irradiation, Pollution Inflict damage to our proteins

An#AgeTreatment

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Aging, UV Irradiation, Pollution Inflict damage to our proteins

An#AgeTreatment

An#-AgingPerformance

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Patents: (EP11305772.3 - PCT/EP2012/061749 - WO2012175519)

Protein carbonyls Detection: OXIPROTEOMICS’ TECHNOLOGY

DIRECT DETECTION

+ Fluorophore

Protein

Protein

+ Fluorophore

Protein

NH2

+ Fluorophore

O Protein

Fluorophore

Detection and quantification of oxidized proteins

using Difference In Gel Electrophoresis: Oxi-DIGE

Protein labelling, automated separation, gel scanning and image analysis

Automated Protein separation, Quantification Analyses and Protein ID

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Our Offer: Carbonyl Score

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Reconstituted skin

Protein Identification

Mass Spectrometry

Efficacy Testing in Dermo Cosmetics

Carbonyl-Score

Oxi-Proteome

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Tape Stripping of human stratum corneum

Protein extraction Starting from 1.5 cm2 of tape stripping surface

Extracted proteins are sufficient for 1 analysis

4µg 8µg 16µg 4µg 8µg 16µg

Intrinsic Aging

Photo Aging

Photo Aging

Intrinsic Aging

Efficacy Testing in Dermo Cosmetics Carbonyl-Score

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Case Study Anti-Pollution Performance

Control COV COV + Actives

Dermal Fibroblasts

COV Volatile Organic

Compounds

COV + Actives

Carbonyl-Score

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Oxi-Proteome

ListofDifferenciallyCarbonylatedSpots(p<0,05) SpotIndex

NormalisedMeanValues Varia#onFactor

Anova (p)

Control COV COV+Ac#fs

952 0,803 2,093 0,809 2,6 0,01 653 1,369 1,720 1,336 1,3 0,01

1409 1,172 1,480 1,155 1,3 0,01 949 0,854 1,264 0,938 1,5 0,01 495 0,506 1,251 0,569 2,5 0,03

802 1,282 1,717 1,259 1,4 0,03

649 1,320 1,797 1,163 1,5 0,03

Case Study Anti-Pollution Performance

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Kit!(in house analyse)! Services!

Quantitative Performance! +! ++++!

Direct Detection! No! Yes!

Assay Variation! High! Minimal!

Optimized and !Validated protocols! No! Yes!

Detection limit! Fentomole! Fentomole!

Time to results! 30 days! 5 days!

Weekly Thrughput! 10 samples! 400 samples!

Competitive Advantages

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Quantitative read-out for : Target identification and molecular mechanisms of protection Quick turn-around of results : Cost Effective :

Final detailed report includes : study description, experimental procedure, results (raw data et HD images) , evaluation and statistical analysis, conclusions.

Scientific objectivation for research and marketing claims

Ø  evaluating ingredients or finished products performance

Ø  development of performant experimental models for efficacy testing

Ø  5 days Carbonyl score Ø  15 days Oxi-Proteome

Ø  Price covers from sample preparation to data ready for communication

Value Proposition

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Bertrand Friguet Scientific Advisor

Martin Baraibar PDG

Claude Hennion Business Developer

Andrea Cavagnino Project Manager

Lucie Brasseur Communications Advisor

The Team

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