Overview on Immunology and Introduction to Innate Immunity
Jan 08, 2018
Overview on Immunology and Introduction to Innate
Immunity
Over view What are the main types of
white blood cells? Name the two main types of
immunity? What are the main distinctions
between these two categories? What cells are involved in which
aspects of the immune system?
Lymphocytes Many types; important
in both humoral and cell-mediated immunity
B-cells produce antibodies (APC cell)
T- cells Cytotoxic T cells Helper T cells
Memory cells
Lymphocytes Natural Killer cells
Large granular lymphocytes (not B or T)
Kills tumor cells Kills cells infected with certain
viruses (intracellular pathogens)
Monocytes/Macrophage Phagocytosis and killing
of microorganisms Activation of T cells and
initiation of immune response
Monocyte is a young macrophage in blood
There are tissue-specific macrophages
APC cells
Dendritic Cells Phagocytosis and killing
of microorganisms Function as antigen
presenting cells (APC) In the blood and tissues
– mature and migrate to the lymph nodes
Neutrophil Granulocyte
Cytoplasmic granules Polymorphonuclear Phagocytosis Short life span (hours) Very important at “clearing” bacterial
infections Innate Immunity
Eosinophils Kills Ab-coated parasites
through degranulation Involved in allergic
inflammation A granulocyte Double Lobed nucleus Orange granules contain
toxic compounds
Basophils Might be “blood Mast
cells’ A cell-killing cells
Blue granules contain toxic and inflammatory compounds
Important in allergic reactions
Antigen-presenting cells (APC) Highly specialized Process antigen and display
peptide fragments on cell surface Involved in T-cell activation Macrophages, dendritic cells and
B-cells
Over view What are the main types of white
blood cells? Name the two main types of
immunity? What are the main distinctions
between these two categories? What cells are involved in which
aspects of the immune system?
Immune system divisions Innate immunity
First line of defense Adaptive (acquired) immunity
Takes time to develop Humoral immunity
(antibody–mediated specific immunity)
Cell-mediated immunity (The aspect of the adaptive immune response where antigen-specific T cell have a main role)
Active immunity
Passive or maternal immunity
Injection of Immunoglobulin
Absorption of maternal antibodies
Innate vs. adaptive immunity
Innate immunity First line of defense (present in all individuals at all
times) Immediate (0 – 4 hours) Non-specific Does not generate lasting protective immunity
Adaptive immune response (late: > 96 hours) Is initiated if innate immune response is not adequate (>
4 days) Antigen-specific immunity Generates lasting protective immunity (e.g. Antibodies,
memory T-cells)
Over view What are the main types of white
blood cells? Name the two main types of
immunity? What are the main distinctions
between these two categories? What white blood cells are
involved in which aspects of the immune system?
Immune system cells Innate immunity
Granulocytes (i.e. neutrophils)
Macrophages Dendritic cells Natural killer (NK)
cells
Adaptive immunity Lymphocyte
B cells T cells
Cytotoxic T cells (CTLs) Helper T cells (Th)
Memory cells
Innate immune system The first line of defense:
Penetration of the epithelial surface of the body by microorganism (e.g. bacteria)
Engulfment of microorganism by macrophages, neutrophils, and dendritic cells
Release of cytokines and chemokines Inflammation
(Immunology animation: Janeway) http://www.blink.biz/immunoanimations/#
Killing by granulocytes Macrophages and neutrophils recognize pathogen by
means of cell-surface receptors Example: mannose receptor, CD14 receptor, scavenger
receptors, glucan receptor etc. Binding of MØ/neutrophils with pathogen leads to
phagocytosis Bound pathogen is surrounded by phagocyte membrane Internalized (phagosome) Killing of pathogen (Phagolysosome*)
Oxidative burst (synthesis of hydrogen peroxide (H2O2)or free oxygen radicals)
Acidification Antimicrobial peptides (e.g. defensins)
* Phagolysosome = lysosome +phagosome
Phagocytosis
The macrophage expresses receptors for
many bacterial constituents
Mannose
receptor
LPS receptor (CD14)
Scavenger
receptor
Bacteria binding to macrophage receptors initiate the release of cytokines and small lipid mediators of
inflammation
Lipid mediators
Cytokines
Lysosome
Phagolysosome
Phagosome
Macrophages engulf and digest bacteria to
which they bind
Phagocytosis (Immunology animation: Janeway)
http://www.blink.biz/immunoanimations/#
Immune response (IV) 9.1 - Phagocytosis
Humoral immune response Cell-surface
immunoglobulin receptors (BCR) detect extracellular pathogens
Once activated, secrete immunoglobulins as soluble antibodies
Antibodies Variable region (2 identical
antigen-binding sites) Constant region (determines
how antibody disposes of the pathogen once it is bound)
Fc region
V region; At binding
Cell killing – NK cells NK cells do not require
prior immunization or activation
They attach to ‘target’ cells (ADCC)
Cytotoxic granules are released onto surface of cell
Effector proteins penetrate cell membrane and induce programmed cell death
Inflammation
Cytokines
Chemokines
FluidsProteins
Bacteria trigger
macrophages to
release cytokines
and chemokines
Vasodilation and increased vascular premeability cause redness, heat, and
swelling
Inflammatory cells migrate into tissue,
releasing inflammatory
mediators that cause pain
Cytokines Low molecular weight, soluble proteins that are
produced in response to an antigen and function as chemical messengers for regulating the innate and adaptive immune system
Innate immune system Macrophages and Dendritic cells
Tumor necrosis factor-alpha (TNF-) Interleukin-1 (IL-1) Interleukin-12 (IL-12)
Adaptive immune system T-lymphocytes
Interleukin-2 (IL-2) Interleukin-4 (IL-4)